Human Glut1 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Glut1 in ELISAs.
  • Source
    Monoclonal Mouse IgG3 Clone # 202921
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with human Glut1
    Met1-Val492
    Accession # AAA52571
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
  • Simple Western
    20 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Glut1 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB14181) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Glut1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Glut1 by Simple WesternTM. Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Glut1 at approximately 69 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB14181). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Glut1

Glut1 belongs to the facilitative glucose transport protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains and is expressed at variable levels in many tissues including brain endothelial cells, CD8+ T cells, and erythrocytes (1‑5). Glut1 is a major glucose transporter that mediates glucose transport across the mammalian blood‑brain barrier.

  • References:
    1. Mueckler, M. et al. 1994, Eur. J. Biochem. 219:713.
    2. Meuckler, M. et al. 1985, Science 229:941.
    3. Jones, K.S. et al. 2006, J. Virol. 8291.
    4. Takenouchi, N. et al. 2007, J. Virol. 1506.
    5. Kinet, S. et al. 2007, Retrovirology 4:31.
  • Long Name:
    Glucose Transporter Type 1
  • Entrez Gene IDs:
    6513 (Human); 20525 (Mouse)
  • Alternate Names:
    DYT17; DYT18; DYT18DYT17; DYT9; EIG12; Glucose transporter type 1, erythrocyte/brain; Glut1; GLUT-1; GLUT1DS; GLUT1GLUT; HepG2 glucose transporter; MGC141895; MGC141896; PED; SLC2A1; solute carrier family 2 (facilitated glucose transporter), member 1; solute carrier family 2, facilitated glucose transporter member 1
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