Detection of Human Glut1 by Western Blot.
Western blot shows lysates of A431 human epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB14181) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Glut1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Glut1 by Simple WesternTM.
Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Glut1 at approximately 69 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Glut1 Monoclonal Antibody (Catalog # MAB14181). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Glut1 belongs to the facilitative glucose transport protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains and is expressed at variable levels in many tissues including brain endothelial cells, CD8+ T cells, and erythrocytes (1‑5). Glut1 is a major glucose transporter that mediates glucose transport across the mammalian blood‑brain barrier.
Mueckler, M. et al. 1994, Eur. J. Biochem. 219:713.
Meuckler, M. et al. 1985, Science 229:941.
Jones, K.S. et al. 2006, J. Virol. 8291.
Takenouchi, N. et al. 2007, J. Virol. 1506.
Kinet, S. et al. 2007, Retrovirology 4:31.
Glucose Transporter Type 1
Entrez Gene IDs:
6513 (Human); 20525 (Mouse)
DYT17; DYT18; DYT18DYT17; DYT9; EIG12; Glucose transporter type 1, erythrocyte/brain; Glut1; GLUT-1; GLUT1DS; GLUT1GLUT; HepG2 glucose transporter; MGC141895; MGC141896; PED; SLC2A1; solute carrier family 2 (facilitated glucose transporter), member 1; solute carrier family 2, facilitated glucose transporter member 1
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