Human Glutathione S-Transferase mu 1/GSTM1 Antibody

Catalog # Availability Size / Price Qty
MAB6894
MAB6894-SP
Detection of Human Glutathione S‑Transferase mu 1/GS by Western Blot.
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Citations (2)
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Human Glutathione S-Transferase mu 1/GSTM1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Glutathione S‑Transferase mu 1/GSTM1 in ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 842516
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Glutathione S‑Transferase mu 1/GSTM1
Met1-Lys218
Accession # P09488
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Glutathione S-Transferase mu 1/GS antibody by Western Blot. View Larger

Detection of Human Glutathione S‑Transferase mu 1/GS by Western Blot. Western blot shows Recombinant Human GSTM1 (Catalog # 6894-GT, 3 ng/lane) and lysates of human adrenal gland tissue and human stomach tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Glutathione S-Transferase mu 1/GS Monoclonal Antibody (Catalog # MAB6894) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Glutathione S-Transferase mu 1/GS at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glutathione S-Transferase mu 1/GSTM1

Glutathione S-Transferases (GSTs) are members of the phase II detoxification enzyme family that conjugate glutathione to various electrophilic compounds, including metabolites generated by oxidative processes in the body, environmental toxins or carcinogens, and anti-cancer drugs. GSTM1 is a cytosolic protein that belongs to the mu class of the GST superfamily. The gene encoding GSTM1 is mapped onto human chromosome 1p13.3 and is known to be highly polymorphic (1). Mostly notably, the widely occurring GSTM1-null genotype has been linked to a variety of cancers including lung (2), gastric (3), bladder (4) and prostate (5). In addition to its role in releasing oxidative stress, GSTM1 has also been suggested to act as a hormone binding protein and play a role in maintaining hormone homeostasis in the body (6, 7).

References
  1. Pearson, W. R. et al. (1993) Am. J. Hum. Genet. 53:220.
  2. Mohr, L.C. et al. (2003) Anticancer. Res. 23:2111.
  3. Wang, H. et al. (2010) Dig. Dis. Sci. 55:1824.
  4. Engel, L. S. et al. (2002) Am. J. Epidermiol. 156:95.
  5. Mo, Z. et al. (2009) Prostate. 69:662.
  6. Mukherjee, S. B. et al. (1999) Biochem. J. 340:309.
  7. Ishigaki, S. et al. (1989) Arch. Biochem. Biophys. 273:265.
Entrez Gene IDs
2944 (Human)
Alternate Names
EC 2.5.1.18; glutathione S-alkyltransferase; glutathione S-aralkyltransferase; glutathione S-aryltransferase; glutathione S-transferase M1; Glutathione STransferase mu 1; Glutathione S-Transferase mu 1; GST class-mu 1; GST HB subunit 4; GST1; GSTM1; GSTM1-1; GSTM1a-1a; GSTM1b-1b; GTH4; GTM1; HB subunit 4; H-B; MGC26563; MU; MU-1; S-(hydroxyalkyl)glutathione lyase

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Citations for Human Glutathione S-Transferase mu 1/GSTM1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. GSTM1 Modulates Expression of Endothelial Adhesion Molecules in Uremic Milieu
    Authors: D Jerotic, S Suvakov, M Matic, A Alqudah, DJ Grieve, M Pljesa-Erc, A Savic-Rado, T Damjanovic, N Dimkovic, L McClements, T Simic
    Oxidative Medicine and Cellular Longevity, 2021;2021(0):6678924.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. A hPSC-based platform to discover gene-environment interactions that impact human ?-cell and dopamine neuron survival
    Authors: T Zhou, TW Kim, CN Chong, L Tan, S Amin, Z Sadat Badi, S Mukherjee, Z Ghazizadeh, H Zeng, M Guo, M Crespo, T Zhang, R Kenyon, CL Robinson, E Apostolou, H Wang, JZ Xiang, T Evans, L Studer, S Chen
    Nat Commun, 2018;9(1):4815.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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