Human Glutathione S-Transferase mu 1/GSTM1 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Glutathione S‑Transferase mu 1/GSTM1 in ELISAs.
  • Source
    Monoclonal Mouse IgG1 Clone # 842516
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human Glutathione S‑Transferase mu 1/GSTM1
    Met1-Lys218
    Accession # P09488
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Glutathione S‑Transferase mu 1/GS by Western Blot. Western blot shows Recombinant Human GSTM1 (Catalog # 6894-GT, 3 ng/lane) and lysates of human adrenal gland tissue and human stomach tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Glutathione S‑Transferase mu 1/GS Monoclonal Antibody (Catalog # MAB6894) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Glutathione S‑Transferase mu 1/GS at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Glutathione S-Transferase mu 1/GSTM1
Glutathione S-Transferases (GSTs) are members of the phase II detoxification enzyme family that conjugate glutathione to various electrophilic compounds, including metabolites generated by oxidative processes in the body, environmental toxins or carcinogens, and anti-cancer drugs. GSTM1 is a cytosolic protein that belongs to the mu class of the GST superfamily. The gene encoding GSTM1 is mapped onto human chromosome 1p13.3 and is known to be highly polymorphic (1). Mostly notably, the widely occurring GSTM1-null genotype has been linked to a variety of cancers including lung (2), gastric (3), bladder (4) and prostate (5). In addition to its role in releasing oxidative stress, GSTM1 has also been suggested to act as a hormone binding protein and play a role in maintaining hormone homeostasis in the body (6, 7).
  • References:
    1. Pearson, W. R. et al. (1993) Am. J. Hum. Genet. 53:220.
    2. Mohr, L.C. et al. (2003) Anticancer. Res. 23:2111.
    3. Wang, H. et al. (2010) Dig. Dis. Sci. 55:1824.
    4. Engel, L. S. et al. (2002) Am. J. Epidermiol. 156:95.
    5. Mo, Z. et al. (2009) Prostate. 69:662.
    6. Mukherjee, S. B. et al. (1999) Biochem. J. 340:309.
    7. Ishigaki, S. et al. (1989) Arch. Biochem. Biophys. 273:265.
  • Entrez Gene IDs:
    2944 (Human)
  • Alternate Names:
    EC 2.5.1.18; glutathione S-alkyltransferase; glutathione S-aralkyltransferase; glutathione S-aryltransferase; glutathione S-transferase M1; Glutathione STransferase mu 1; Glutathione S-Transferase mu 1; GST class-mu 1; GST HB subunit 4; GST1; GSTM1; GSTM1-1; GSTM1a-1a; GSTM1b-1b; GTH4; GTM1; HB subunit 4; H-B; MGC26563; MU; MU-1; S-(hydroxyalkyl)glutathione lyase
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

Ctrl MAB002 138  
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Mouse IgG1 Isotype Control

Ctrl MAB002R 1
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse IgG Antibody

WB AF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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