Human Glypican 4 Antibody

    
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  • Species Reactivity
    Human
  • Specificity
    Detects human Glypican 4 in direct ELISAs.
  • Source
    Monoclonal Mouse IgG1 Clone # 961609
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human Glypican 4
    Met1-Ser529
    Accession # O75487
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • ELISA

    This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human Glypican 4 Monoclonal Antibody (Catalog # MAB91951).

    This product is intended for assay development on various assay platforms requiring antibody pairs.

  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Glypican 4 in Human TF-1 Cell Line by Flow Cytometry. TF‑1 human erythroleukemic cell line was stained with Mouse Anti-Human Glypican 4 Monoclonal Antibody (Catalog # MAB9195, filled histogram) or Mouse IgG1 Isotype Control (Catalog # MAB002, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
Detection of Glypican 4 in Human BG01V Cells by Flow Cytometry. BG01V human embryonic stem cells were stained with Mouse Anti-Human Glypican 4 Monoclonal Antibody (Catalog # MAB9195, filled histogram) or Mouse IgG1 Isotype Control (Catalog # MAB002, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Glypican 4
Glypican 4, also known as K-Glypican, is an approximately 220 kDa GPI-anchored heparan sulfate proteoglycan with a 60 kDa protein core (1, 2). It is expressed in brain, kidney, adrenal gland, and fat tissue (1, 3) and binds to basic FGF (2). Mature human Glypican 4 shares 97% and 96% amino acid (aa) sequence identity with mouse and rat Glypican 4, respectively. An alternative splice isoform lacks the N-terminal 70 aa including the signal peptide. In the developing brain, Glypican 4 is found in lateral ventricles surrounding the telencephalon, the dentate gyrus, proliferating neuroepithelial cells, and neural precursors (1, 2). It inhibits the dopaminergic differentiation of neurons (4). A 30 kDa cleaved form of Glypican 4 binds in cis to PTP sigma and contributes to excitatory synapse development and function (5). Glypican 4 is differentially expressed between adipose tissue depots (3). A soluble form can be released by adipocytes and circulates at elevated levels in obese patients with insulin resistance (3, 6). This form binds and enhances signaling through the Insulin R, and it also supports adipocyte differentiation (3). In zebrafish, Glypican 4 is required for cartilage formation and cardiomyocyte differentiation (7, 8).
  • References:
    1. Watanabe, K. et al. (1995) J. Cell Biol. 130:1207.
    2. Hagihara, K. et al. (2000) Dev. Dyn. 219:353.
    3. Ussar, S. et al. (2012) Diabetes 61:2289.
    4. Fico, A. et al. (2014) J. Neurosci. 34:8318.
    5. Ko, J.S. et al. (2015) Proc. Natl. Acad. Sci. USA 112:1874.
    6. Zhu, H.J. et al. (2014) J. Endocrinol. Metab. 99:E2697.
    7. Sisson, B.E. et al. (2015) Mech. Dev. 138:279.
    8. Strate, I. et al. (2015) Development 142:1767.
  • Entrez Gene IDs:
    2239 (Human); 14735 (Mouse); 317322 (Rat)
  • Alternate Names:
    Glypican 4; glypican proteoglycan 4; glypican-4; GPC4; K-glypican; K-glypicandJ900E8.1 (glypican 4)
Related Research Areas

FAQs

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Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

Ctrl MAB002 148  
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Mouse IgG1 Isotype Control

Ctrl MAB002R 1
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Reconstitution Buffers
Description Application Cat# Citations Images  

Reconstitution Buffer 1 (PBS)

RB01
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 32  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 12  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow , IHC , ICC NL007 10
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow , IHC , ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 4  
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG Antibody

WB AF007 4
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow , IHC , ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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