Human GM-CSF R alpha Antibody

Catalog # Availability Size / Price Qty
MAB706
MAB706-SP
Product Details
Citations (1)
FAQs
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Human GM-CSF R alpha Antibody Summary

Species Reactivity
Human
Specificity
Detects human GM-CSF R alpha in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) M‑CSF R or rhGM‑CSF R beta  ( beta c) is observed.
Source
Monoclonal Mouse IgG1 Clone # 31916
Purification
Protein A or G purified from ascites
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human GM-CSF R alpha
Extracellular domain
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human GM-CSF R alpha (Catalog # 706-GR)
Flow Cytometry
2.5 µg/106 cells
Human peripheral blood monocytes
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GM-CSF R alpha

Granulocyte macrophage colony stimulating factor receptor alpha (GM-CSF R alpha ), also known as CD116, is a component of the receptor complex that mediates cellular responses to GM-CSF. GM-CSF promotes the differentiation and mobilization of granulocyte-macrophage, erythroid, megakaryocyte, and eosinophil progenitors. It enhances the activation of myeloid cell effector functions and plays a role in the development of Th1 biased immune responses, allergic inflammation, and autoimmunity (1‑4). Mature human GM-CSF R alpha is an 80 kDa type I transmembrane glycoprotein that consists of a 298 amino acid (aa) extracellular domain (ECD) with two fibronectin type III domains and a juxtamembrane WSxWS motif, a 26 aa transmembrane segment, and a 54 aa cytoplasmic domain (5). Within the ECD, human GM-CSF R alpha shares approximately 33% aa sequence identity with mouse and rat GM-CSF R alpha. Alternative splicing of human GM-CSF R alpha generates several additional isoforms that lack the cytoplasmic and/or transmembrane regions. Soluble forms of the receptor retain the ability to bind GM-CSF (6, 7). GM-CSF R alpha is expressed on hematopoietic stem cells, progenitor and differentiated cells in the myeloid lineage, vascular endothelial cells, placenta, and non-hematopoietic solid tumor cells (8). GM-CSF R alpha associates with the common beta chain/CD131 ( beta c), a 135 kDa transmembrane protein that is also the signal transducing component of the receptors for IL-3 and IL-5 (9, 10). Association with beta c converts GM-CSF R alpha from a low affinity to a high affinity receptor for GM-CSF (9‑11). The shared usage of beta c underlies the synergism between GM-CSF, IL-3, and IL-5 in their effects on myeloid cell differentiation and activation (1, 2).

References
  1. Martinez-Moczygemba, M. and D.P. Huston (2003) J. Allergy Clin. Immunol. 112:653.
  2. Fleetwood, A.J. et al. (2005) Crit. Rev. Immunol. 25:405.
  3. Eksioglu, E.A. et al. (2007) Exp. Hematol. 35:1163.
  4. Cao, Y. (2007) J. Clin. Invest. 117:2362.
  5. Gearing, D.P. et al. (1989) EMBO J. 8:3667.
  6. Pelley, J.L. et al. (2007) Exp. Hematol. 35:1483.
  7. Raines, M.A. et al. (1991) Proc. Natl. Acad. Sci. USA 88:8203.
  8. Chiba, S. et al. (1990) Cell Regul. 1:327.
  9. Kitamura, T. et al. (1991) Proc. Natl. Acad. Sci. USA 88:5082.
  10. Hayashida, K. et al. (1990) Proc. Natl. Acad. Sci. USA 87:9655.
  11. Hoang, T. et al. (1993) J. Biol. Chem. 268:11881.
Long Name
Granulocyte Macrophage Growth Factor Receptor alpha
Entrez Gene IDs
1438 (Human); 12982 (Mouse); 102139775 (Cynomolgus Monkey)
Alternate Names
CD116 antigen; CD116; CDw116; colony stimulating factor 2 receptor alpha subunit; colony stimulating factor 2 receptor, alpha, low-affinity(granulocyte-macrophage); CSF2R; CSF2RA; CSF2RAX; CSF2RAY; CSF2RX; CSF2RY; GM-CSF R alpha; GM-CSF receptor alpha subunit; GMCSFR alpha; GMCSFR; GM-CSFRa; GM-CSF-R-alpha; GMCSFR-alpha; GMR; GMR-alpha; granulocyte-macrophage colony-stimulating factor receptor alpha chain; granulocyte-macrophage colony-stimulating factor receptor subunit alpha; MGC3848; MGC4838

Product Datasheets

Citation for Human GM-CSF R alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Inhibition of purinergic P2X receptor 7 (P2X7R) decreases granulocyte-macrophage colony-stimulating factor (GM-CSF) expression in U251 glioblastoma cells
    Authors: M Drill, KL Powell, LK Kan, NC Jones, TJ O'Brien, JA Hamilton, M Monif
    Sci Rep, 2020;10(1):14844.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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