Human GPR115 Antibody

Catalog # Availability Size / Price Qty
AF5437
AF5437-SP
Detection of Human GPR115 by Western Blot.
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Human GPR115 Antibody Summary

Species Reactivity
Human
Specificity
Detects human GPR115 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) GPR111, rhGPR114, and rhGPR125 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human GPR115
Ser22-Ala347
Accession # Q8IZF3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human GPR115 antibody by Western Blot. View Larger

Detection of Human GPR115 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human GPR115 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5437) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for GPR115 at approximately 100-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPR115

GPR115 is a member of the LN-7TM family of adhesion-type 7-transmembrane (TM) G-protein coupled receptors (GPCR) that show a long extracellular N-terminus (1, 2). The 695 amino acid (aa) human GPR115 sequence predicts a 21 aa signal sequence, a 385 aa N‑terminal extracellular domain (ECD), seven TM regions separated by 6‑24 aa intracellular and extracellular regions, and a 40 aa cytoplasmic tail. Like other LN-7TM members, the ECD contains a highly glycosylated mucin‑like stalk that is predicted to function in adhesion. This is followed by a cysteine-rich GPCR proteolytic cleavage site (GPS) (1). GPS domains, which have been described in other 7TM proteins including ETL, GPR126, HE6, and Latrophilin-1, are cleavage sites for processing proteins into two subunits (3‑7). Within the N‑terminal region that ends with the predicted cleavage site (aa 22‑347), human GPR115 shares 58% aa sequence identity with the corresponding region of mouse and rat GPR115. GPR115 was identified from expressed sequence tags (ESTs) found in pregnant uterus, breast, and the genitourinary tract (1).

References
  1. Fredriksson, R. et al. (2002) FEBS Lett. 531:407.
  2. Bjarnadottir, T.K. et al. (2004) Genomics 84:23.
  3. Nechiporuk, T. et al. (2001) J. Biol. Chem. 276:4150.
  4. Moriguchi, T. et al. (2004) Genes Cells 9:549.
  5. Kierszenbaum, A.L. (2003) Mol. Reprod. Dev. 64:1.
  6. Krasnoperov, V.G. et al. (1997) Neuron 18:925.
  7. Krasnoperov, V. et al. (2002) J. Biol. Chem. 277:46518.
Long Name
G Protein-coupled Receptor 115
Entrez Gene IDs
221393 (Human); 78249 (Mouse); 501106 (Rat)
Alternate Names
FLJ38076; G protein-coupled receptor 115; GPR115; G-protein coupled receptor PGR18; PGR18; PGR18G-protein coupled receptor 115; probable G-protein coupled receptor 115; seven transmembrane helix receptor

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human GPR115 Antibody

There are currently no reviews for this product. Be the first to review Human GPR115 Antibody and earn rewards!

Have you used Human GPR115 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review