Human GPR37 Antibody

Catalog # Availability Size / Price Qty
MAB4450
MAB4450-SP

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Detection of GPR37 in A172 Human Cell Line by Flow Cytometry.
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Human GPR37 Antibody Summary

Species Reactivity
Human
Specificity
Detects human GPR37.
Source
Monoclonal Mouse IgG2A Clone # 436923
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human GPR37
Ala27-Cys613
Accession # O15354
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of GPR37 antibody in A172 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of GPR37 in A172 Human Cell Line by Flow Cytometry. A172 human glioblastoma cell line was stained with Mouse Anti-Human GPR37 Monoclonal Antibody (Catalog # MAB4450, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPR37

GPR37 (G-protein coupled receptor 37), also called ETBR-LP-1 (endothelin B receptor-like protein 1) or PAELR (Parkin-associated endothelin receptor-like receptor) is a 613 aa, ~55 kDa orphan 7-transmembrane receptor. It is mainly expressed in neuronal cells, particularly in cerebellar Purkinje cells and the hippocampus. It is a substrate of the E3 ubiquitin ligase, parkin, which is upregulated during endoplasmic reticulum stress. In a juvenile form of Parkinson’s disease, GPR37 accumulates, contributing to stress-induced neuronal cell death. The extracellular portions of human and mouse GPR37 share 68% aa identity.

Long Name
G Protein-coupled Receptor 37
Entrez Gene IDs
2861 (Human); 14763 (Mouse); 117549 (Rat)
Alternate Names
EDNRBL; Endothelin B receptor-like protein 1; ETBR-LP-1; G protein-coupled receptor 37 (endothelin receptor type B-like); GPR37; hET(B)R-LP; PAELR; PAELRETBR-LP-1; Parkin-associated endothelin receptor-like receptor; probable G-protein coupled receptor 37

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