Detection of GPR37 in A172 Human Cell Line by Flow Cytometry. A172 human glioblastoma cell line was stained with Mouse Anti-Human GPR37 Monoclonal Antibody (Catalog # MAB4450, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
GPR37 (G-protein coupled receptor 37), also called ETBR-LP-1 (endothelin B receptor-like protein 1) or PAELR (Parkin-associated endothelin receptor-like receptor) is a 613 aa, ~55 kDa orphan 7-transmembrane receptor. It is mainly expressed in neuronal cells, particularly in cerebellar Purkinje cells and the hippocampus. It is a substrate of the E3 ubiquitin ligase, parkin, which is upregulated during endoplasmic reticulum stress. In a juvenile form of Parkinson’s disease, GPR37 accumulates, contributing to stress-induced neuronal cell death. The extracellular portions of human and mouse GPR37 share 68% aa identity.
G Protein-coupled Receptor 37
Entrez Gene IDs:
2861 (Human); 14763 (Mouse); 117549 (Rat)
EDNRBL; Endothelin B receptor-like protein 1; ETBR-LP-1; G protein-coupled receptor 37 (endothelin receptor type B-like); GPR37; hET(B)R-LP; PAELR; PAELRETBR-LP-1; Parkin-associated endothelin receptor-like receptor; probable G-protein coupled receptor 37
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