Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 689705
View all GPR75 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human GPR75
Accession # O95800

Specificity

Detects human GPR75 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human GPR75 Antibody

GPR75 antibody in Human Brain by Immunohistochemistry (IHC-P).

GPR75 in Human Brain.

GPR75 was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Mouse Anti-Human GPR75 Monoclonal Antibody (Catalog # MAB6728) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human GPR75 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (hippocampus)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GPR75

GPR75 is a 540 amino acid, 7-transmembrane G-protein-coupled glycoprotein receptor mainly expressed in retinal pigment epithelial cells and brain. Polymorphisms in humans have been associated with macular degeneration. GPR75 has been identified as a receptor for CCL5/RANTES in the brain. Collectively, the extracellular domains of human GPR75 share 75% amino acid sequence identity with the corresponding regions of mouse and rat GPR75.

Long Name

G Protein-coupled Receptor 75

Alternate Names

GPRchr2

Entrez Gene IDs

10936 (Human); 237716 (Mouse); 498434 (Rat)

Gene Symbol

GPR75

UniProt

O95800

Additional GPR75 Products

Product Documents for Human GPR75 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human GPR75 Antibody

For research use only

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Protocols

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