Human GPR75 Antibody

Catalog # Availability Size / Price Qty
MAB67281-100
MAB67281-SP
Detection of GPR75 in HEK293 Human Cell Line Transfected with Human GPR75 and eGFP by Flow Cytometry.
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Human GPR75 Antibody Summary

Species Reactivity
Human
Specificity
Detects human GPR75 in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 689714
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human GPR75
Accession # O95800
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
HEK293 Human Cell Line Transfected with Human GPR75 and eGFP

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry View Larger

Detection of GPR75 in HEK293 Human Cell Line Transfected with Human GPR75 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human GPR75 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human GPR75 Monoclonal Antibody (Catalog # MAB67281) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on Mouse IgG2A Isotype Control (MAB003). Staining was performed using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPR75

GPR75 is a 540 amino acid, 7-transmembrane G-protein-coupled glycoprotein receptor mainly expressed in retinal pigment epithelial cells and brain. Polymorphisms in humans have been associated with macular degeneration. GPR75 has been identified as a receptor for CCL5/RANTES in the brain. Collectively, the extracellular domains of human GPR75 share 75% amino acid sequence identity with the corresponding regions of mouse and rat GPR75.

Long Name
G Protein-coupled Receptor 75
Entrez Gene IDs
10936 (Human); 237716 (Mouse); 498434 (Rat)
Alternate Names
G protein-coupled receptor 75; GPR75; GPRchr2; MGC132002; probable G-protein coupled receptor 75; WI31133; WI-31133

Product Datasheets

FAQs

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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