Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat, Transgenic Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry, ELISA Capture

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all HAPLN1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human HAPLN1 (R&D Systems, Catalog # 2608-HP)
Asp16-Asn354
Accession # P10915

Specificity

Detects human HAPLN1 in direct ELISAs and Western blots. In these formats, this antibody shows approximately 5% cross‑reactivity with recombinant human HAPLN4.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human HAPLN1 Antibody

Detection of Human HAPLN1 by Immunocytochemistry/Immunofluorescence

Detection of Human HAPLN1 by Immunocytochemistry/Immunofluorescence

Absent or fragmented omnipause neuron perineuronal net triple immunofluorescence staining.Triple immunofluorescence staining for different components of perineuronal nets, revealed by a confocal laser scanning microscope. In the control case, omnipause neurons (OPN) are ensheathed by prominent perineuronal nets showing the same appearance with antibodies against the link protein (HPLN1), chondroitin sulfate proteoglycan (CSPG) and aggrecan (ACAN) (A, D, G, arrow). In the saccadic palsy patient, the neurons of the superior olive (SO) from the same sections as OPN are ensheathed by prominent perineuronal nets revealed by immunostaining of HPLN1, CSPG, and ACAN (C, F, I, arrows). However, around OPN (asterisk) in the patient, only HPLN1-based perineuronal nets can be detected, which appear fragmented (B, arrow). CSPG- and ACAN-immunostaining does not reveal perineuronal nets, but only few fragments along a few dendrites (E, H, arrow). Scale bars A,D,G = 20μm; B,C,E,F,H,I = 200μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26135580), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human HAPLN1 by Immunocytochemistry/Immunofluorescence

Detection of Human HAPLN1 by Immunocytochemistry/Immunofluorescence

Absent or fragmented omnipause neuron perineuronal net triple immunofluorescence staining.Triple immunofluorescence staining for different components of perineuronal nets, revealed by a confocal laser scanning microscope. In the control case, omnipause neurons (OPN) are ensheathed by prominent perineuronal nets showing the same appearance with antibodies against the link protein (HPLN1), chondroitin sulfate proteoglycan (CSPG) and aggrecan (ACAN) (A, D, G, arrow). In the saccadic palsy patient, the neurons of the superior olive (SO) from the same sections as OPN are ensheathed by prominent perineuronal nets revealed by immunostaining of HPLN1, CSPG, and ACAN (C, F, I, arrows). However, around OPN (asterisk) in the patient, only HPLN1-based perineuronal nets can be detected, which appear fragmented (B, arrow). CSPG- and ACAN-immunostaining does not reveal perineuronal nets, but only few fragments along a few dendrites (E, H, arrow). Scale bars A,D,G = 20μm; B,C,E,F,H,I = 200μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26135580), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human HAPLN1 by Immunocytochemistry/Immunofluorescence

Detection of Human HAPLN1 by Immunocytochemistry/Immunofluorescence

Absent or fragmented omnipause neuron perineuronal net triple immunofluorescence staining.Triple immunofluorescence staining for different components of perineuronal nets, revealed by a confocal laser scanning microscope. In the control case, omnipause neurons (OPN) are ensheathed by prominent perineuronal nets showing the same appearance with antibodies against the link protein (HPLN1), chondroitin sulfate proteoglycan (CSPG) and aggrecan (ACAN) (A, D, G, arrow). In the saccadic palsy patient, the neurons of the superior olive (SO) from the same sections as OPN are ensheathed by prominent perineuronal nets revealed by immunostaining of HPLN1, CSPG, and ACAN (C, F, I, arrows). However, around OPN (asterisk) in the patient, only HPLN1-based perineuronal nets can be detected, which appear fragmented (B, arrow). CSPG- and ACAN-immunostaining does not reveal perineuronal nets, but only few fragments along a few dendrites (E, H, arrow). Scale bars A,D,G = 20μm; B,C,E,F,H,I = 200μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26135580), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human HAPLN1 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human HAPLN1 (Catalog # 2608-HP)

Reviewed Applications

Read 2 reviews rated 5 using AF2608 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HAPLN1

HAPLN1 (also known as link protein and CRTL1) is a member of the hyaladherin family of hyaluronic acid (HA) binding proteins. Hyaluronan binding proteins are of two types; those with link modules, and those without. Link modules are 100 amino acid (aa) HA and protein-binding sequences that contain two alpha -helices and two antiparallel beta -sheets (1, 3). There are three categories of link module-containing proteins. “A” domain-type proteins contain one link module; “B” domain-type proteins contain one link module with an N- and C-terminal flanking region; and “C” domain-type proteins have an extended structure with one N-terminal V-type Ig-like domain followed by two link modules (2). The HAPLN family is a group of four C domain-type proteins that share approximately 50% aa identity (4). HAPLN1 is synthesized as a 354 aa precursor that contains a 15 aa signal sequence and a 339 aa mature region (4 - 6). It contains one Ig-like domain and two 95 aa link modules (6). It is variably glycosylated with a native molecular weight between 41 - 48 kDa (7, 8). Mature human HAPLN1 is 97%, 96%, 96%, 96%, and 96% aa identical to equine, porcine, rat, mouse and bovine HAPLN1, respectively. HAPLN1 contributes to extracellular matrix stability and flexibility (9). In cartilage, HALPN1 forms a ternary complex with HA and aggrecan. This creates a gel-like substance with remarkable resistance to deformation (3). In this complex, HA forms a linear backbone with perpendicularly attached aggrecan and HAPLN1. Aggrecan and HAPLN1 lie parallel to each other, while HA runs between the two HAPLN1 link modules (2, 3, 10). The Ig domain of HAPLN1 binds to aggrecan, while the two link modules of HAPLN1 bind to HA. Although HA and aggrecan will associate, the tendency is towards dissociation (2, 3, 8). HAPLN1 provides a stabilizing influence on HA-aggrecan associations, thus creating a long-lived ternary functional complex.

References

  1. Day, A.J. and G.D. Prestwich (2002) J. Biol. Chem. 277:4585.
  2. Seyfried, N.T. et al. (2005) J. Biol. Chem. 280:5435.
  3. Matsumoto, K. et al. (2003) J. Biol. Chem. 278:41205.
  4. Spicer, A.P. et al. (2003) J. Biol. Chem. 278:21083.
  5. Dudhia, J. and T.E. Hardingham (1990) Nucleic Acids Res. 18:1292.
  6. Osborne-Lawrence, S.L. et al. (1990) Genomics 8:562.
  7. Roughley, P.J. et al. (1982) J. Biol. Chem. 257:11908.
  8. Shi, S. et al. (2004) J. Biol. Chem. 279:12060.
  9. Binette, F. et al. (1994) J. Biol. Chem. 269:19116.
  10. Perkins, S.J. et al. (1992) Biochem. J. 285:263.

Long Name

Hyaluronan and Proteoglycan Link Protein 1

Alternate Names

CRTL1

Entrez Gene IDs

1404 (Human)

Gene Symbol

HAPLN1

UniProt

P10915

Additional HAPLN1 Products

Product Documents for Human HAPLN1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human HAPLN1 Antibody

For research use only

Citations for Human HAPLN1 Antibody

Customer Reviews for Human HAPLN1 Antibody (2)

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2 Customer Ratings
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Customer Images

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  • Human HAPLN1 Antibody
    Name: Kimberly Alonge
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Adult brain
    Species: Rat and Mouse
    Verified Customer | Posted 04/10/2018
    Very strong and specific staining.
    Human HAPLN1 Antibody AF2608
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 23940048
    Species: Rat
    Verified Customer | Posted 01/07/2015

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