Human Heparan Sulfate 2-O-Sulfotransferase 1/HS2ST1 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Heparan Sulfate 2-O-Sulfotransferase 1/HS2ST1 in ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 738306
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human Heparan Sulfate 2-O-Sulfotransferase 1/HS2ST1
    Met59-Asn356
    Accession # Q7LGA3
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Heparan Sulfate 2-O-Sulfotransferase 1/HS2ST1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and SH‑SY5Y human neuroblastoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Heparan Sulfate 2-O-Sulfotransferase 1/HS2ST1 Monoclonal Antibody (Catalog # MAB6335) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Heparan Sulfate 2-O-Sulfotransferase 1/HS2ST1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Heparan Sulfate 2-O-Sulfotransferase 1/HS2ST1
Heparan sulfate is a highly sulfated polysaccharide found on the cell surface and within the extracellular matrix. Typically, it is covalently attached to the protein core of proteoglycans, such as syndecans and glypicans. Heparin, on the other hand, can be considered as a highly sulfated version of heparan sulfate that is predominantly found in mast cells. Both heparin and heparan sulfate contain disaccharide repeats of uronic acid and N‑acetylglucosamine and are modified by the same sulfotransferases (1, 2). The uronic acid residues are either glucuronic acid or iduronic acid and maybe sulfated at the 2-O position by heparan sulfate 2‑O sulfotransferase 1 (HS2ST1) (3, 4). HS2ST1 physically interacts in the Golgi apparatus with glucuronyl c5-epimerase (5), which catalyzes the conversion of glucuronic acid to iduronic acid (6). As a consequence, 2-O sulfation predominantly occurs on iduronic acids naturally and overexpression of HS2ST1 alone causes an increase in 2-O sulfation on glucuronic acid (7).
  • References:
    1. Bernfield, M. et al. (1999) Annu. Rev. Biochem. 68:729.
    2. Esko, J.D. and Selleck, S.B. (2002) Annu. Rev. Biochem. 71:435.
    3. Kobayashi, M. et al. (1996) J. Biol. Chem. 271:7645.
    4. Bullock, S.L. et al. (1998) Genes & Development 12:1894.
    5. Li, J.P. et al. (2001) J. Biol. Chem. 276:20069.
    6. Pinhal, M.A.S et al. (2001) Proc. Natl. Acad. Sci. USA. 98:12984.
    7. Rong, J. et al. (2000) Biochem. J. 346:463.
  • Entrez Gene IDs:
    9653 (Human)
  • Alternate Names:
    2OST; 2-O-sulfotransferase; EC 2.8.2; EC 2.8.2.-; FLJ11317; heparan sulfate 2-O-sulfotransferase 1; HS2ST; HS2ST1; KIAA0448dJ604K5.2; MGC131986
Related Research Areas
Isotype Controls
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Mouse IgG1 Isotype Control

Ctrl MAB002R 1
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Secondary Antibodies
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Mouse IgG HRP-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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