Human Heparan Sulfate 2-O-Sulfotransferase 1/HS2ST1 Antibody (738306)

R&D Systems | Catalog # MAB6335

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 738306
View all Heparan Sulfate 2-O-Sulfotransferase 1/HS2ST1 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Heparan Sulfate 2-O-Sulfotransferase 1/HS2ST1
Met59-Asn356
Accession # Q7LGA3

Specificity

Detects human Heparan Sulfate 2-O-Sulfotransferase 1/HS2ST1 in ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Heparan Sulfate 2-O-Sulfotransferase 1/HS2ST1 Antibody (738306)

Detection of Human Heparan Sulfate 2-O-Sulfotransferase 1/HS2ST1 antibody by Western Blot.

Detection of Human Heparan Sulfate 2-O-Sulfotransferase 1/HS2ST1 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and SH-SY5Y human neuroblastoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Heparan Sulfate 2-O-Sulfotransferase 1/HS2ST1 Monoclonal Antibody (Catalog # MAB6335) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Heparan Sulfate 2-O-Sulfotransferase 1/HS2ST1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Heparan Sulfate 2-O-Sulfotransferase 1/HS2ST1 Antibody (738306)

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and SH‑SY5Y human neuroblastoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Heparan Sulfate 2-O-Sulfotransferase 1/HS2ST1

Heparan sulfate is a highly sulfated polysaccharide found on the cell surface and within the extracellular matrix. Typically, it is covalently attached to the protein core of proteoglycans, such as syndecans and glypicans. Heparin, on the other hand, can be considered as a highly sulfated version of heparan sulfate that is predominantly found in mast cells. Both heparin and heparan sulfate contain disaccharide repeats of uronic acid and N‑acetylglucosamine and are modified by the same sulfotransferases (1, 2). The uronic acid residues are either glucuronic acid or iduronic acid and maybe sulfated at the 2-O position by heparan sulfate 2‑O sulfotransferase 1 (HS2ST1) (3, 4). HS2ST1 physically interacts in the Golgi apparatus with glucuronyl c5-epimerase (5), which catalyzes the conversion of glucuronic acid to iduronic acid (6). As a consequence, 2-O sulfation predominantly occurs on iduronic acids naturally and overexpression of HS2ST1 alone causes an increase in 2-O sulfation on glucuronic acid (7).

References

  1. Bernfield, M. et al. (1999) Annu. Rev. Biochem. 68:729.
  2. Esko, J.D. and Selleck, S.B. (2002) Annu. Rev. Biochem. 71:435.
  3. Kobayashi, M. et al. (1996) J. Biol. Chem. 271:7645.
  4. Bullock, S.L. et al. (1998) Genes & Development 12:1894.
  5. Li, J.P. et al. (2001) J. Biol. Chem. 276:20069.
  6. Pinhal, M.A.S et al. (2001) Proc. Natl. Acad. Sci. USA. 98:12984.
  7. Rong, J. et al. (2000) Biochem. J. 346:463.

Alternate Names

2OST

Entrez Gene IDs

9653 (Human)

Gene Symbol

HS2ST1

UniProt

Q7LGA3

Additional Heparan Sulfate 2-O-Sulfotransferase 1/HS2ST1 Products

Product Documents for Human Heparan Sulfate 2-O-Sulfotransferase 1/HS2ST1 Antibody (738306)

Certificate of Analysis

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Product Specific Notices for Human Heparan Sulfate 2-O-Sulfotransferase 1/HS2ST1 Antibody (738306)

For research use only

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Associated Pathways

Articular Cartilage Extracellular Matrix
Articular Cartilage Extracellular Matrix Thumbnail