Human HPRG Antibody

Catalog # Availability Size / Price Qty
AF1869
AF1869-SP
Product Details
Citations (1)
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Human HPRG Antibody Summary

Species Reactivity
Human
Specificity
Detects human HPRG in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant mouse HPRG is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human HPRG
Val19-Lys525
Accession # P04196
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human HPRG (Catalog # 1869-HP)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HPRG

Human histidine‑rich glycoprotein (HPRG) is a multidomain, monomeric, secreted, 67‑75 kDa member of the cystatin superfamily of molecules (1, 2). Its name derives from the fact that 26% of its amino acids (aa) are histidine and proline. In human, it is synthesized as a 525 aa precursor that contains an 18 aa signal sequence and a 507 aa mature region (3). Five distinct domains are recognized in the mature molecule. There are two N‑terminal cystatin‑like modules (aa 19‑254) and one His‑Pro‑rich region (aa 350‑497) that is flanked by two Pro‑rich segments (aa 276‑321 and 498‑525) (3, 4). The His‑Pro‑rich region contains 10 tandem repeats with an HHPHG motif, and the N‑ and C‑termini are linked by a disulfide bond (3, 5, 6). Human HPRG is only 60% aa identical to mouse HPRG. There are multiple ligands for HPRG. These include small molecular weight molecules (metal ions; heme), hemostatic molecules (heparan sulfate; TSP; plasminogen), and immune system components (T cells; macrophages) (1, 5). About 50% of plasma plasminogen circulates bound to HPRG. Upon immobilization to cell surface tropomyosin in a Zn++‑dependent manner, it is converted to plasmin by tPA (7‑9). HPRG also shows antiangiogenic activity on endothelial cells (10).

References
  1. Jones, A.L. et al. (2005) Immunol. Cell Biol. 83:106.
  2. Koide, T. and S. Odani (1987) FEBS Lett. 216:17.
  3. Koide, T. et al. (1986) Biochemistry 25:2220.
  4. SwissProt Accession # P04196.
  5. Borza, D-B. et al. (1996) Biochemistry 35:1925.
  6. Sorensen, C.B. et al. (1993) FEBS Lett. 328:285.
  7. Donate, F. et al. (2004) Cancer Res. 64:5812.
  8. Borza, D-B. and W.T. Morgan (1997) J. Biol. Chem. 272:5718.
  9. Guan, X. et al. (2004) Thromb. Haemost. 92:403.
  10. Juarez, J.C. et al. (2002) Cancer Res. 62:5344.
Long Name
Histidine-rich Glycoprotein Precusor
Entrez Gene IDs
3273 (Human)
Alternate Names
DKFZp779H1622; Histidine-proline-rich glycoprotein; histidine-rich glycoprotein; HPRG; HPRGHRGP; HRG

Product Datasheets

Citation for Human HPRG Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Levels of caspase-3 and histidine-rich glycoprotein in the embryo secretome as biomarkers of good-quality day-2 embryos and high-quality blastocysts
    Authors: H Kaihola, FG Yaldir, T Bohlin, R Samir, J Hreinsson, H Åkerud
    PLoS ONE, 2019;14(12):e0226419.
    Species: Human
    Sample Types: Tissue Culture Supernates
    Applications: Proximity Extension Assay

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