Human ICAT Antibody

Catalog # Availability Size / Price Qty
AF3016
AF3016-SP
Product Details
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Human ICAT Antibody Summary

Species Reactivity
Human
Specificity
Detects human ICAT in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human ICAT
Met1-Gln81
Accession # Q9NSA3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human ICAT

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICAT

Human ICAT is a physiological inhibitor of the beta -Catenin/Wnt signaling pathway. It is 9 kDa in size and found in both cytosol and nucleus. The molecule contains an alpha ‑helical N-terminal region (aa 9‑54) and an acidic, extended C-terminal region (aa 55‑78). The alpha -helical region binds to beta -Catenin, displacing CBP/p300, while the acidic C-terminus interacts with the beta -Catenin N-terminus, blocking Tcf interaction. Human ICAT shares 97% aa sequence identity with mouse and bovine ICAT, respectively.

Long Name
Beta-Catenin-Interacting Protein
Entrez Gene IDs
56998 (Human)
Alternate Names
beta-catenin-interacting protein ICAT; catenin, beta interacting protein 1; CTNNBIP1; ICAT; ICATbeta-interacting protein 1; Inhibitor of beta-catenin and Tcf-4

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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