Human IFN-alpha / beta R1 Antibody

Catalog #: AF245
7 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF245
AF245-SP

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Detection of Human IFN‑ alpha / beta R1 by Western Blot.

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Citations (7)

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Human IFN-alpha / beta R1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human IFN‑ alpha / beta R1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse IFN‑ alpha / beta RI is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IFN-alpha / beta R1
Lys28-Lys436
Accession # AAA52730

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1-1 µg/mL

Recombinant Human IFN-alpha / beta R1, HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line

Flow Cytometry

2.5 µg/10⁶ cells

U937 human histiocytic lymphoma cell line

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human IFN‑ alpha / beta R1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IFN‑ alpha / beta R1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF245) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for IFN‑ alpha / beta R1 at approximately 110-120 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-alpha/beta R1

Type I interferons (IFN-alpha, IFN-beta, IFN-omega ) bind to the type I IFN receptor, also called the IFN alpha/beta receptor. This receptor is composed of two chains, IFN-alpha / beta R1 and R2.

Long Name

Interferon alpha/beta Receptor 1

Entrez Gene IDs

3454 (Human); 15975 (Mouse); 101926190 (Cynomolgus Monkey)

Alternate Names

alpha-type antiviral protein; AVP; beta-type antiviral protein; CRF2-1; Cytokine receptor class-II member 1; Cytokine receptor family 2 member 1; human interferon-alpha receptor (HuIFN-alpha-Rec)10IFRC; IFN-alpha/beta R1; IFN-alpha/beta receptor 1; IFN-alpha-REC; IFNAR; IFNAR1; IFN-aR1; IFNBR; IFNbR1; IFN-bR1; IFN-R-1; interferon (alpha, beta and omega) receptor 1; interferon alpha/beta receptor 1; interferon-alpha/beta receptor alpha chain; interferon-beta receptor 1; Type I interferon receptor 1

Product Datasheets

Product Datasheet

COA

SDS

Product Specific Notices

Sold under license from Pestka Biomedical Laboratories, Inc. d/b/a PBL Interferon Source. For research use only. Not for resale. Not for diagnostic, therapeutic or clinical purposes.

Related Research Areas

Citations for Human IFN-alpha / beta R1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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A human iPSC-array-based GWAS identifies a virus susceptibility locus in the NDUFA4 gene and functional variants
Authors: Y Han, L Tan, T Zhou, L Yang, L Carrau, LA Lacko, M Saeed, J Zhu, Z Zhao, BE Nilsson-Pa, FT Lira Neto, C Cahir, AM Giani, JC Chai, Y Li, X Dong, D Moroziewic, NYSCF Glob, D Paull, T Zhang, S Koo, C Tan, R Danziger, Q Ba, L Feng, Z Chen, A Zhong, GJ Wise, JZ Xiang, H Wang, RE Schwartz, BR tenOever, SA Noggle, CM Rice, Q Qi, T Evans, S Chen
Cell Stem Cell, 2022-10-06;29(10):1475-1490.e6.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Trisomy 21 consistently activates the interferon response
Elife, 2016-07-29;5(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Chronic exposure to type-I IFN under lymphopenic conditions alters CD4 T cell homeostasis.
Authors: Le Saout C, Hasley R, Imamichi H, Tcheung L, Hu Z, Luckey M, Park J, Durum S, Smith M, Rupert A, Sneller M, Lane H, Catalfamo M
PLoS Pathog, 2014-03-06;10(3):e1003976.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Murine plasmacytoid dendritic cells produce IFN-gamma upon IL-4 stimulation.
Authors: Suto A, Nakajima H, Tokumasa N, Takatori H, Kagami S, Suzuki K, Iwamoto I
J. Immunol., 2005-11-01;175(9):5681-9.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
Type I and type II interferons delay human neutrophil apoptosis via activation of STAT3 and up-regulation of cellular inhibitor of apoptosis 2.
Authors: Sakamoto E, Hato F, Kato T, Sakamoto C, Akahori M, Hino M, Kitagawa S
J. Leukoc. Biol., 2005-04-21;78(1):301-9.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Blocking of the alpha interferon-induced Jak-Stat signaling pathway by Japanese encephalitis virus infection.
Authors: Lin RJ, Liao CL, Lin E, Lin YL
J. Virol., 2004-09-01;78(17):9285-94.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Autocrine type I IFN and contact with endothelium promote the presentation of influenza A virus by monocyte-derived APC.
Authors: Qu C, Moran TM, Randolph GJ
J. Immunol., 2003-01-15;170(2):1010-8.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization