Human IFN-alpha 2 Antibody

Catalog # Availability Size / Price Qty
MAB93455-100
MAB93455-SP
IFN‑ alpha 2  in Human PBMCs.
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Human IFN-alpha 2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IFN‑ alpha 2 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 995510
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
HEK293 human embryonic kidney cell line transfected with human IFN‑ alpha 2
Met1-Glu188
Accession # P01563
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry IFN‑ alpha 2  in Human PBMCs. View Larger

IFN‑ alpha 2 in Human PBMCs. IFN‑ alpha 2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human IFN‑ alpha 2 Monoclonal Antibody (Catalog # MAB93455) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-alpha 2

Interferon-alpha 2 (IFNA2), also known as leukocyte interferon, is a type I interferon  produced by macrophages,  CD8 resting T cells, tonsillar NK cells, germinal center B cells, as well as epithelial cells, tumor cells and melanocytes. IFN alpha has at least nine different isoforms.

IFNA2 is a ligand for a cell surface receptor with two subunits, IFN-alpha R2 (ligand binding) and IFN-alpha R1 (ligand binding and signal transduction). IFNA2 has both antiviral and immunomodulatory activities on target cells.  Intranasal administration has been shown to reduce viral load in subjects with the common cold. While IFNA2 is a prognostic factor for hepatocellular carcinoma, Interferon alpha 2b, has been used to treat melanoma, renal cell carcinoma, chronic myelogenous leukemia and hepatitis C.

References
  1. Chen, Zy et al. (2014) Br. J. Cancer 110:733.
  2. Burchert, A. et al. (2015) Leukemia 29:1331.
  3. Perrakis, A. et al. (2011) Transplant Proc. 43:3824.
  4. Akaza, H. et al. (2011) Jpn. J. Clin. Oncol. 41:1023.
Long Name
Interferon alpha 2
Entrez Gene IDs
3440 (Human); 15965 (Mouse); 298213 (Rat)
Alternate Names
alpha-2a interferon; Ifa2; IFNA; IFNA2; IFNA2b; IFNalpha 2; IFN-alpha 2; IFN-alpha-2; IFN-alphaA; INFA2; interferon alpha 2b; interferon alpha A; interferon alpha-2; Interferon alpha-A; interferon, alpha 2; LeIF A; MGC125764; MGC125765

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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