Human IFN-beta Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
IFN-beta Inhibition of EMCV-inducedCytopathy andNeutralization by HumanIFN-beta Antibody.
1 Image
Product Details
Supplemental Products

Human IFN-beta Antibody Summary

Species Reactivity
Detects human IFN‑ beta in direct ELISAs.
Recombinant Monoclonal Mouse IgG1 Clone # 76703R
Protein A or G purified from cell culture supernatant
E. coli-derived recombinant human IFN‑ beta
Accession # P01574
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.


Recommended Concentration
Measured by its ability to neutralize IFN-beta inhibition of EMCV-inducedcytopathy in the HeLa human cervicalepithelial carcinoma cell line. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens,M.J. et al. (eds): IRL Press. 129. The Neutralization Dose (ND50) is typically 7-21μg/mL in the presence of50 pg/mL Recombinant Human IFN-beta.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization IFN-beta Inhibition of EMCV-inducedCytopathy andNeutralization by HumanIFN-beta Antibody. View Larger

IFN-beta Inhibition of EMCV-inducedCytopathy andNeutralization by Human
IFN-beta Antibody.
Recombinant HumanIFN-beta (Catalog # 8499-IF)reduces theEncephalomyocarditis Virus(EMCV)-inducedcytopathy in theHeLa human cervical epithelialcarcinoma cell line in a dose-dependentmanner (orange line),as measured by crystal violetstaining. Inhibition of EMCVactivity elicited by RecombinantHuman IFN-beta (50 pg/mL) isneutralized (green line) byincreasing concentrations of Recombinant Human IFN-beta MonoclonalAntibody (Catalog # MAB814R).The ND50 is typically 7-21μg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-beta

Interferon beta (IFN-beta ) is synthesized and secreted by fibroblasts and many other cell types in response to pathogens. IFN-beta binding to type I interferon receptors induces the upregulation of IRF-7 and activation of Rnase L. IRF-7 can exert a positive feedback on IFN-beta production. RNase L cleaves both viral and cellular single stranded mRNA, thereby limiting viral replication and dissemination.

Long Name
Interferon beta
Entrez Gene IDs
3456 (Human); 15977 (Mouse); 24481 (Rat)
Alternate Names
Fibroblast interferon; IFBIFNB; IFF; IFNB1; IFNbeta; IFN-beta; interferon beta; interferon, beta 1, fibroblast; MGC96956

Product Datasheets


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human IFN-beta Antibody

There are currently no reviews for this product. Be the first to review Human IFN-beta Antibody and earn rewards!

Have you used Human IFN-beta Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review