Protein A or G purified from cell culture supernatant
E. coli-derived recombinant human IFN‑ beta Met22-Asn187 Accession # P01574
as a solution in PBS. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Measured by its ability to neutralize IFN-beta inhibition of EMCV-inducedcytopathy in the HeLa human cervicalepithelial carcinoma cell line. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens,M.J. et al. (eds): IRL Press. 129. The Neutralization Dose (ND50) is typically 7-21μg/mL in the presence of50 pg/mL Recombinant Human IFN-beta.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
IFN-beta Inhibition of EMCV-inducedCytopathy andNeutralization by Human IFN-beta Antibody. Recombinant HumanIFN-beta (Catalog # 8499-IF)reduces theEncephalomyocarditis Virus(EMCV)-inducedcytopathy in theHeLa human cervical epithelialcarcinoma cell line in a dose-dependentmanner (orange line),as measured by crystal violetstaining. Inhibition of EMCVactivity elicited by RecombinantHuman IFN-beta (50 pg/mL) isneutralized (green line) byincreasing concentrations of Recombinant Human IFN-beta MonoclonalAntibody (Catalog # MAB814R).The ND50 is typically 7-21μg/mL.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C, as supplied.
1 month, 2 to 8 °C under sterile conditions after opening.
6 months, -20 to -70 °C under sterile conditions after opening.
Interferon beta (IFN-beta ) is synthesized and secreted by fibroblasts and many other cell types in response to pathogens. IFN-beta binding to type I interferon receptors induces the upregulation of IRF-7 and activation of Rnase L. IRF-7 can exert a positive feedback on IFN-beta production. RNase L cleaves both viral and cellular single stranded mRNA, thereby limiting viral replication and dissemination.
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