Human IFN-beta Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB814R-100
MAB814R-SP
MAB814R-500
MAB814R-01M
IFN-beta Inhibition of EMCV-inducedCytopathy andNeutralization by HumanIFN-beta Antibody.
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Human IFN-beta Antibody Summary

Species Reactivity
Human
Specificity
Detects human IFN‑ beta in direct ELISAs.
Source
Recombinant Monoclonal Mouse IgG1 Clone # 76703R
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E. coli-derived recombinant human IFN‑ beta
Met22-Asn187
Accession # P01574
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Neutralization
Measured by its ability to neutralize IFN-beta inhibition of EMCV-inducedcytopathy in the HeLa human cervicalepithelial carcinoma cell line. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens,M.J. et al. (eds): IRL Press. 129. The Neutralization Dose (ND50) is typically 7-21μg/mL in the presence of50 pg/mL Recombinant Human IFN-beta.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization IFN-beta Inhibition of EMCV-inducedCytopathy andNeutralization by HumanIFN-beta Antibody. View Larger

IFN-beta Inhibition of EMCV-inducedCytopathy andNeutralization by Human
IFN-beta Antibody.
Recombinant HumanIFN-beta (Catalog # 8499-IF)reduces theEncephalomyocarditis Virus(EMCV)-inducedcytopathy in theHeLa human cervical epithelialcarcinoma cell line in a dose-dependentmanner (orange line),as measured by crystal violetstaining. Inhibition of EMCVactivity elicited by RecombinantHuman IFN-beta (50 pg/mL) isneutralized (green line) byincreasing concentrations of Recombinant Human IFN-beta MonoclonalAntibody (Catalog # MAB814R).The ND50 is typically 7-21μg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-beta

Interferon beta (IFN-beta ) is synthesized and secreted by fibroblasts and many other cell types in response to pathogens. IFN-beta binding to type I interferon receptors induces the upregulation of IRF-7 and activation of Rnase L. IRF-7 can exert a positive feedback on IFN-beta production. RNase L cleaves both viral and cellular single stranded mRNA, thereby limiting viral replication and dissemination.

Long Name
Interferon beta
Entrez Gene IDs
3456 (Human); 15977 (Mouse); 24481 (Rat)
Alternate Names
Fibroblast interferon; IFBIFNB; IFF; IFNB1; IFNbeta; IFN-beta; interferon beta; interferon, beta 1, fibroblast; MGC96956

Product Datasheets

FAQs

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Isotype Controls

Mouse IgG1 Isotype Control

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Reconstitution Buffers

Secondary Antibodies

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