Human IFN-beta Antibody

Catalog # Availability Size / Price Qty
21400-1
Product Details
Citations (3)
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Human IFN-beta Antibody Summary

Species Reactivity
Human
Specificity
Binds to human interferon beta with high affinity. For best results in experiments involving neutralization of the anti-viral effects of human interferon beta we recommend using Sheep Polyclonal Antibody to human interferon beta (Product 31400-1).
Source
Polyclonal Mouse IgG1
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human interferon beta
Formulation
Phosphate-buffered saline (PBS) containing 0.1% bovine serum albumin (BSA)
Label
Unconjugated

Applications

Recommended Concentration
Sample
Neutralization
One neutralization unit is the amount of antiserum required to neutralize one unit of human interferon beta (Hu-IFN-beta ) to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect inhibition assay as described [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," J. Virol. 37, 755-758; Familletti, P.C., Rubinstein, S., and Pestka, S. (1981) "A Convenient and Rapid Cytopathic Effect Inhibition Assay for Interferon," in Methods in Enzymology, Vol. 78 (S. Pestka, ed.), Academic Press, New York, 387-394]. In this antiviral assay for interferon about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined with respect to the international reference standard for Hu-IFN-beta provided by the National Institutes of Health [see Pestka, S. (1986) "Interferon Standards and General Abbreviations," in Methods in Enzymology (S. Pestka, ed.), Academic Press, New York 119, 14-23].

Due to the variation in ND50 (Neutralizing Dose) values based on the cell type and assay system, we recommend each user determine the neutralizing concentration of this antibody lot in their assay system. Using an A549/EMCV (cell/virus) system, we have not verified with reasonable consistency the neutralizing concentration of this antibody (the concentration required to inhibit the antiviral effect of human interferon beta by one half).

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
After receipt, the product may be stored at -20°C for short-term use ( ≤ 6 months). For long-term storage, we recommend storing the product at -70°C or below for retention of full activity. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum. Refreezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of the product should be in buffers containing protein such as 0.1% bovine serum albumin (BSA).

Background: IFN-beta

Interferon-beta (IFN-beta) is a cytokine that is produced by fibroblasts and pathogen-exposed dendritic cells, macrophages, and endothelial cells. It signals through the heterodimeric IFN-alpha/beta Receptor. IFN-beta-deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS.

Long Name
Interferon beta
Entrez Gene IDs
3456 (Human); 15977 (Mouse); 24481 (Rat)
Alternate Names
Fibroblast interferon; IFBIFNB; IFF; IFNB1; IFNbeta; IFN-beta; interferon beta; interferon, beta 1, fibroblast; MGC96956

Product Datasheets

Citations for Human IFN-beta Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. poly(I:C) and LPS induce distinct IRF3 and NF-kappaB signaling during type-I IFN and TNF responses in human macrophages.
    Authors: Reimer T, Brcic M, Schweizer M
    J. Leukoc. Biol., 2008;83(5):1249-57.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Neutralizing type I IFN antibodies trigger an IFN-like response in endothelial cells.
    Authors: Moll HP, Freudenthaler H, Zommer A
    J. Immunol., 2008;180(8):5250-6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Type I IFN induction in response to Listeria monocytogenes in human macrophages: evidence for a differential activation of IFN regulatory factor 3 (IRF3).
    Authors: Reimer T, Schweizer M, Jungi TW
    J. Immunol., 2007;179(2):1166-77.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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