Human IFN-beta DuoSet ELISA

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DY814-05
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Human IFN-beta ELISA Standard Curve
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Product Details
Procedure
Citations (38)
FAQs
Supplemental Products
Reviews (10)

Human IFN-beta DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Range
7.8 - 500 pg/mL
Sufficient Materials
For five 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Interferon beta (IFN-β). The suggested diluent is suitable for the analysis of most cell culture supernate, serum, and plasma samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data

Human IFN-beta ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IFN-beta

Interferon-beta (IFN-beta) is a cytokine that is produced by fibroblasts and pathogen-exposed dendritic cells, macrophages, and endothelial cells. It signals through the heterodimeric IFN-alpha/beta Receptor. IFN-beta-deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS.

Long Name:
Interferon beta
Entrez Gene IDs:
3456 (Human); 15977 (Mouse); 24481 (Rat)
Alternate Names:
Fibroblast interferon; IFB; IFBIFNB; IFF; IFNB; IFNB1; IFNbeta; IFN-beta; interferon beta; interferon, beta 1, fibroblast; MGC96956

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL of Block Buffer to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Human IFN-beta DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

38 Citations: Showing 1 - 10
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  1. Serum interleukin-6, procalcitonin, and C-reactive protein at hospital admission can identify patients at low risk for severe COVID-19 progression
    Authors: Zobel, CM;Wenzel, W;Krüger, JP;Baumgarten, U;Wagelöhner, T;Neumann, N;Foroutan, B;Müller, R;Müller, A;Rauschning, D;Schü beta ler, M;Scheit, L;Weinreich, F;Oltmanns, K;Keidel, F;Koch, M;Spethmann, S;Schreiner, M;
    Frontiers in microbiology
    Species: Human
    Sample Types: Serum
  2. Met receptor is essential for MAVS-mediated antiviral innate immunity in epithelial cells independent of its kinase activity
    Authors: Imamura, R;Sato, H;Chih-Cheng Voon, D;Shirasaki, T;Honda, M;Kurachi, M;Sakai, K;Matsumoto, K;
    Proceedings of the National Academy of Sciences of the United States of America
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Development of cyclopeptide inhibitors of cGAS targeting protein-DNA interaction and phase separation
    Authors: Wang, X;Wang, Y;Cao, A;Luo, Q;Chen, D;Zhao, W;Xu, J;Li, Q;Bu, X;Quan, J;
    Nature communications
    Species: Human
    Sample Types: Cell Culture Supernates
  4. Development of LB244, an Irreversible STING Antagonist
    Authors: Barasa, L;Chaudhuri, S;Zhou, JY;Jiang, Z;Choudhary, S;Green, RM;Wiggin, E;Cameron, M;Humphries, F;Fitzgerald, KA;Thompson, PR;
    Journal of the American Chemical Society
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Complete substitution with modified nucleotides suppresses the early interferon response and increases the potency of self-amplifying RNA
    Authors: McGee, JE;Kirsch, JR;Kenney, D;Chavez, E;Shih, TY;Douam, F;Wong, WW;Grinstaff, MW;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Cell Culture Supernates
  6. ZIKV induction of tristetraprolin in endothelial and Sertoli cells post-transcriptionally inhibits IFN?/? expression and promotes ZIKV persistence
    Authors: Schutt, WR;Conde, JN;Mladinich, MC;Himmler, GE;Mackow, ER;
    mBio
    Species: Human
    Sample Types: Cell Culture Supernates
  7. TNF? induced by DNA-sensing in macrophage compromises retinal pigment epithelial (RPE) barrier function
    Authors: Twarog, M;Schustak, J;Xu, Y;Coble, M;Dolan, K;Esterberg, R;Huang, Q;Saint-Geniez, M;Bao, Y;
    Scientific reports
    Species: Human
    Sample Types: Cell Culture Supernates, Cell Lysates
  8. Adipokine levels and their association with clinical disease severity in patients with dengue
    Authors: Kuruppu, H;Wickramanayake, WPRH;Jeewandara, C;Peranantharajah, D;Colambage, HS;Perera, L;Gomes, L;Wijewickrama, A;Ogg, GS;Malavige, GN;
    PLoS neglected tropical diseases
    Species: Human
    Sample Types: Serum
  9. Flow-Based CL-SMIA for the Quantification of Protein Biomarkers from Nasal Secretions in Comparison with Sandwich ELISA
    Authors: Neumair, J;Kröger, M;Stütz, E;Jerin, C;Chaker, AM;Schmidt-Weber, CB;Seidel, M;
    Biosensors
    Species: Human
    Sample Types: Nasal Lining Fluid
  10. SARS-CoV-2 Nsp2 Contributes to Inflammation by Activating NF-kappaB
    Authors: É Lacasse, L Gudimard, I Dubuc, A Gravel, I Allaeys, É Boilard, L Flamand
    Viruses, 2023-01-24;15(2):.
    Species: Human
    Sample Types: Cell Culture Supernates
  11. Blood leukocyte transcriptional modules and differentially expressed genes associated with disease severity and age in COVID-19 patients
    Authors: SY Bando, FB Bertonha, SE Vieira, DBL de Oliveir, VN Chalup, EL Durigon, P Palmeira, ACP Curi, CS Faria, L Antonangel, GDP Lauterbach, FA Regalio, RM Cesar, CA Moreira-Fi
    Scientific Reports, 2023-01-17;13(1):898.
    Species: Human
    Sample Types: Plasma
  12. Association between the expression of Toll-like receptors, cytokines, and homeostatic chemokines in SARS-CoV-2 infection and COVID-19 severity
    Authors: W Alturaiki, H Alkadi, S Alamri, ME Awadalla, A Alfaez, A Mubarak, MA Alanazi, FQ Alenzi, BF Flanagan, B Alosaimi
    Heliyon, 2022-12-24;0(0):e12653.
    Species: Human
    Sample Types: Serum
  13. The Golgi-resident protein ACBD3 concentrates STING at ER-Golgi contact sites to drive export from the ER
    Authors: K Motani, N Saito-Tara, K Nishino, S Yamauchi, N Minakawa, H Kosako
    Cell Reports, 2022-12-20;41(12):111868.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. PRC2-mediated epigenetic suppression of type I IFN-STAT2 signaling impairs antitumor immunity in luminal breast cancer
    Authors: J Hong, JH Lee, Z Zhang, Y Wu, M Yang, Y Liao, R de la Rosa, J Scheirer, D Pechacek, N Zhang, Z Xu, T Curiel, X Tan, TH Huang, K Xu
    Cancer Research, 2022-12-16;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  15. Structure-activity relationship study of amidobenzimidazole derivatives as stimulator of interferon genes (STING) agonists
    Authors: X Liu, M Wang, M Yang, H Sun, B Wang, X Pan, X Chen, J Jin, X Wang
    European Journal of Medicinal Chemistry, 2022-11-24;246(0):114943.
    Species: Human
    Sample Types: Cell Culture Supernates
  16. Signal strength of STING activation determines cytokine plasticity and cell death in human monocytes
    Authors: D Kabelitz, M Zarobkiewi, M Heib, R Serrano, M Kunz, G Chitadze, D Adam, C Peters
    Scientific Reports, 2022-10-24;12(1):17827.
    Species: Human
    Sample Types: Cell Culture Supernates
  17. Pro-Inflammatory Cytokines and Interferon-Stimulated Gene Responses Induced by Seasonal Influenza A Virus with Varying Growth Capabilities in Human Lung Epithelial Cell Lines
    Authors: AA Hinay, S Kakee, S Kageyama, A Tsuneki-To, WY Perdana, Y Akena, S Nishiyama, K Kanai
    Vaccines, 2022-09-09;10(9):.
    Species: Human
    Sample Types: Cell Lysates
  18. Deactylation by SIRT1 enables liquid-liquid phase separation of IRF3/IRF7 in innate antiviral immunity
    Authors: Z Qin, X Fang, W Sun, Z Ma, T Dai, S Wang, Z Zong, H Huang, H Ru, H Lu, B Yang, S Lin, F Zhou, L Zhang
    Nature Immunology, 2022-07-25;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  19. Distinct airway epithelial immune responses after infection with SARS-CoV-2 compared to H1N1
    Authors: H Stölting, L Baillon, R Frise, K Bonner, RJ Hewitt, PL Molyneaux, ML Gore, Breathing, WS Barclay, S Saglani, CM Lloyd
    Mucosal Immunology, 2022-07-15;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  20. Decreased leukocyte exhaustion is associated with decreased IFN-beta and increased alpha-defensin-1 levels in type-2 diabetes
    Authors: S Shruthi, JM Sibi, V Mohan, S Babu, V Nirmaladev, V Aravindhan
    Cytokine, 2022-05-28;156(0):155918.
    Species: Human
    Sample Types: Cell Culture Supernates
  21. Green and Roasted Coffee Extracts Inhibit Interferon-beta Release in LPS-Stimulated Human Macrophages
    Authors: V Artusa, C Ciaramelli, A D'Aloia, FA Facchini, N Gotri, A Bruno, B Costa, A Palmioli, C Airoldi, F Peri
    Frontiers in Pharmacology, 2022-05-05;13(0):806010.
    Species: Human
    Sample Types: Cell Culture Supernates
  22. Translocated Legionella pneumophila small RNAs mimic eukaryotic microRNAs targeting the host immune response
    Authors: T Sahr, P Escoll, C Rusniok, S Bui, G Pehau-Arna, G Lavieu, C Buchrieser
    Nature Communications, 2022-02-09;13(1):762.
    Species: Human
    Sample Types: Cell Culture Supernates
  23. Development of Small-Molecule STING Activators for Cancer Immunotherapy
    Authors: HR Jung, S Jo, MJ Jeon, H Lee, Y Chu, J Lee, E Kim, GY Song, C Jung, H Kim, S Lee
    Biomedicines, 2021-12-24;10(1):.
    Species: Human
    Sample Types: Cell Culture Supernates
  24. Downregulation of calcium-regulated heat stable protein 1 expression by low-temperature stimulation causes reduction of interferon-beta expression and sensitivity to influenza viral infection
    Authors: K Nishioka, T Daidoji, T Nakaya
    Virus research, 2021-12-17;0(0):198659.
    Species: Human
    Sample Types: Cell Culture Supernates
  25. Cross-species analysis of viral nucleic acid interacting proteins identifies TAOKs as innate immune regulators
    Authors: FL Pennemann, A Mussabekov, C Urban, A Stukalov, LL Andersen, V Grass, TM Lavacca, C Holze, L Oubraham, Y Benamrouch, E Girardi, RE Boulos, R Hartmann, G Superti-Fu, M Habjan, JL Imler, C Meignin, A Pichlmair
    Nature Communications, 2021-12-01;12(1):7009.
    Species: Human
    Sample Types: Cell Culture Supernates
  26. The Endogenous RIG-I Ligand Is Generated in Influenza A-Virus Infected Cells
    Authors: J Steinberg, T Wadenpohl, S Jung
    Viruses, 2021-08-07;13(8):.
    Species: Human
    Sample Types: Cell Culture Supernates
  27. Genetic and chemical inhibition of IRF5 suppresses pre-existing mouse lupus-like disease
    Authors: T Ban, M Kikuchi, GR Sato, A Manabe, N Tagata, K Harita, A Nishiyama, K Nishimura, R Yoshimi, Y Kirino, H Yanai, Y Matsumoto, S Suzuki, H Hihara, M Ito, K Tsukahara, K Yoshimatsu, T Yamamoto, T Taniguchi, H Nakajima, S Ito, T Tamura
    Nature Communications, 2021-07-19;12(1):4379.
    Species: Human
    Sample Types: Cell Culture Supernates
  28. Paracrine IFN Response Limits ZIKV Infection in Human Sertoli Cells
    Authors: DP Strange, B Jiyarom, H Sadri-Arde, LH Cazares, TA Kenny, MD Ward, S Verma
    Frontiers in Microbiology, 2021-05-17;12(0):667146.
    Species: Human
    Sample Types: Cell Culture Supernates
  29. Extracellular vesicles promote transkingdom nutrient transfer during viral-bacterial co-infection
    Authors: MR Hendricks, S Lane, JA Melvin, Y Ouyang, DB Stolz, JV Williams, Y Sadovsky, JM Bomberger
    Cell Reports, 2021-01-26;34(4):108672.
    Species: Human
    Sample Types: Cell Culture Supernates
  30. PPP6C Negatively Regulates STING-Dependent Innate Immune Responses
    Authors: G Ni, Z Ma, JP Wong, Z Zhang, E Cousins, MB Major, B Damania
    MBio, 2020-08-04;11(4):.
    Species: Human
    Sample Types: Cell Culture Supernates
  31. Major 5'terminally deleted enterovirus populations modulate type I IFN response in acute myocarditis patients and in human cultured cardiomyocytes
    Authors: M Glenet, Y N'Guyen, A Mirand, C Henquell, AL Lebreil, F Berri, F Bani-Sadr, B Lina, I Schuffenec, L Andreolett
    Sci Rep, 2020-07-20;10(1):11947.
    Species: Human
    Sample Types: Cell Culture Supernates
  32. MG53 suppresses interferon-&beta and inflammation via regulation of ryanodine receptor-mediated intracellular calcium signaling
    Authors: M Sermershei, AD Kenney, PH Lin, TM McMichael, C Cai, K Gumpper, TMA Adesanya, H Li, X Zhou, KH Park, JS Yount, J Ma
    Nat Commun, 2020-07-17;11(1):3624.
    Species: Human
    Sample Types: Cell Culture Supernates
  33. Axl Promotes Zika Virus Entry and Modulates the Antiviral State of Human Sertoli Cells
    Authors: DP Strange, B Jiyarom, N Pourhabibi, X Xie, C Baker, H Sadri-Arde, PY Shi, S Verma
    MBio, 2019-07-16;10(4):.
    Species: Human
    Sample Types: Cell Culture Supernates
  34. MicroRNA-122 supports robust innate immunity in hepatocytes by targeting the RTKs/STAT3 signaling pathway
    Authors: H Xu, SJ Xu, SJ Xie, Y Zhang, JH Yang, WQ Zhang, MN Zheng, H Zhou, LH Qu
    Elife, 2019-02-08;8(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  35. Human polyomavirus BKV infection of endothelial cells results in interferon pathway induction and persistence
    Authors: P An, MT Sáenz Robl, AM Duray, PG Cantalupo, JM Pipas
    PLoS Pathog., 2019-01-08;15(1):e1007505.
    Species: Human
    Sample Types: Cell Culture Supernates
  36. An Anti-Inflammatory Azaphenothiazine Inhibits Interferon ? Expression and CXCL10 Production in KERTr Cells
    Authors: L Strzadala, A Fiedorowic, E Wysokinska, E Ziolo, M Grudzie?, M Jelen, K Pluta, B Morak-Mlod, M Zimecki, W Kalas
    Molecules, 2018-09-24;23(10):.
    Species: Human
    Sample Types: Cell Culture Supernates
  37. The effects of CD14 and IL-27 on induction of endotoxin tolerance in human monocytes and macrophages
    Authors: C Petes, V Mintsopoul, RL Finnen, BW Banfield, K Gee
    J. Biol. Chem., 2018-09-21;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  38. Zika Virus Persistently Infects and Is Basolaterally Released from Primary Human Brain Microvascular Endothelial Cells
    Authors: MC Mladinich, J Schwedes, ER Mackow
    MBio, 2017-07-11;8(4):.
    Species: Human
    Sample Types: Cell Culture Supernates

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Reviews for Human IFN-beta DuoSet ELISA

Average Rating: 4.4 (Based on 10 Reviews)

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Human IFN-beta DuoSet ELISA
By Anonymous on 06/23/2023
Sample Tested: HEK293 cell line,HEK293 human embryonic kidney cell line

Human IFN-beta DuoSet ELISA
By Anonymous on 12/03/2021
Sample Tested: Serum-free Cell Culture Supernates

Human IFN-beta DuoSet ELISA
By Anonymous on 10/21/2021
Sample Tested: Serum-free Cell Culture Media

Human IFN-beta DuoSet ELISA
By Anonymous on 06/09/2021
Sample Tested: Adult lung

Human IFN-beta DuoSet ELISA
By Helen Stoelting on 04/29/2021
Sample Tested: Cell culture supernatant

Human IFN-beta DuoSet ELISA
By Anonymous on 11/09/2020
Sample Tested: THP-1 human acute monocytic leukemia cell line

THP-1 cells were stimulated with cytosolic nucleic acids for 18 hours at 1microgram/ml.


Human IFN-beta DuoSet ELISA
By Anonymous on 08/05/2020
Sample Tested: Cell Culture Media

Human IFN-beta DuoSet ELISA
By Anonymous on 04/02/2020
Sample Tested: Serum and Plasma

We used this kit for the quantification of INF b in human serum and plasma. Works very well and well-described protocol.


Human IFN-beta DuoSet ELISA
By Anonymous on 05/01/2019
Sample Tested: Jurkat human acute T cell leukemia cell line,MOLT-4 human acute lymphoblastic leukemia cell line

Human IFN-beta DuoSet ELISA
By Kim Han on 01/03/2019
Sample Tested: Cell culture supernatant