Human IL-10 R alpha Antibody

Catalog # Availability Size / Price Qty
MAB274-SP
MAB274-100
MAB274-500
IL‑10 Inhibition of IL‑1 beta  secretion and Neutralization by Human IL‑10 R alpha  Antibody.
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Product Details
Citations (10)
FAQs
Supplemental Products
Reviews (1)

Human IL-10 R alpha Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-10 R alpha in direct ELISAs. In this format, this antibody does not cross-react with recombinant mouse IL‑10 R alpha.
Source
Monoclonal Mouse IgG1 Clone # 37607
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑10 R alpha
His22-Asn235
Accession # Q13651.1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Neutralization
Measured by its ability to neutralize IL‑10 R alpha -mediated IL‑10 response in LPS-activated human peripheral blood mononuclear cells (PBMC). Ralph, P. et al. (1991) J. Immunology 148:808. The Neutralization Dose (ND50) is typically 0.03-0.1 µg/mL in the presence of 0.25 ng/mL Recombinant Human IL‑10 and 0.25 ng/mL LPS.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization IL‑10 Inhibition of IL‑1 beta  secretion and Neutralization by Human IL‑10 R alpha  Antibody. View Larger

IL‑10 Inhibition of IL‑1 beta secretion and Neutralization by Human IL‑10 R alpha Antibody. Recombinant Human IL‑10 (Catalog # 217-IL) inhibits IL‑1 beta secretion in LPS-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line), as measured by the Human IL‑1 beta /IL‑1F2 Quantikine ELISA Kit (Catalog # DLB50). IL‑1 beta secretion inhibited by Recombinant Human IL‑10 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Human IL‑10 R alpha Monoclonal Antibody (Catalog # MAB274). The ND50 is typically 0.03-0.1 µg/mL in the presence of LPS (0.25 ng/mL).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-10 R alpha

IL-10, initially designated cytokine synthesis inhibitory factor (CSIF), is a potent immunosuppressant of macrophage functions. IL-10 is also a pleiotropic cytokine with multiple immunostimulatory as well as immunosuppressive effects on a variety of other cell types. IL-10 binds specifically and with high affinity to cell-surface receptors. Mouse and human cDNA clones encoding the ligand-binding IL-10 receptor (IL-10 R) have been isolated. The IL-10 R mRNA has been detected in all cell types that are known to respond to IL-10.

Human and mouse IL-10 receptors are structurally related to the IFN-gamma receptor. These receptors are members of the class II subgroup of the cytokine receptor superfamily. The deduced amino acid sequence of human IL-10 R is approximately 60% identical to mouse IL-10 R. Although human IL-10 has cross-species activities and is active on mouse cells, mouse IL-10 is species-specific in its actions and does not bind to the human IL-10 receptor. The human IL-10 R gene has been mapped to chromosome 11q23.3. Recombinant IL-10 soluble receptor, consisting of the extracellular domain of IL-10 R, binds IL-10 with high affinity in solution and is a potent IL-10 antagonist.

Long Name
Interleukin 10 Receptor alpha
Entrez Gene IDs
3587 (Human); 16154 (Mouse); 102127536 (Cynomolgus Monkey)
Alternate Names
CD210 antigen; CDw210a; HIL-10R; IBD28; IL-10 R alpha; IL-10 receptor subunit alpha; IL10R alpha; IL-10R subunit 1; IL-10R1; IL10RA; IL-10Ra; IL10RIL-10R subunit alpha; interleukin 10 receptor, alpha; interleukin-10 receptor alpha chain; Interleukin-10 receptor subunit 1; interleukin-10 receptor subunit alpha

Product Datasheets

Citations for Human IL-10 R alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Opposing regulation of the late phase TNF response by mTORC1-IL-10 signaling and hypoxia in human macrophages
    Sci Rep, 2016;6(0):31959.
    Species: Human
    Sample Types: Whole Cells
    Applications: Blocking
  2. IL-10 Receptor Signaling Is Essential for TR1 Cell Function In Vivo
    Authors: Leonie Brockmann
    J. Immunol, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  3. Interleukin-10 receptor-1 expression in monocyte-derived antigen-presenting cell populations: dendritic cells partially escape from IL-10's inhibitory mechanisms.
    Authors: von Lanzenauer S, Wolk K, Hoflich C, Kunz S, Grunberg B, Docke W, Reineke U, Asadullah K, Sterry W, Volk H, Sabat R
    Genes Immun, 2015;16(1):8-14.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. TNF-alpha blockade induces IL-10 expression in human CD4+ T cells.
    Authors: Evans H, Roostalu U, Walter G, Gullick N, Frederiksen K, Roberts C, Sumner J, Baeten D, Gerwien J, Cope A, Geissmann F, Kirkham B, Taams L
    Nat Commun, 2014;5(0):3199.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. IFNgamma/IL-10 co-producing cells dominate the CD4 response to malaria in highly exposed children.
    Authors: Jagannathan P, Eccles-James I, Bowen K, Nankya F, Auma A, Wamala S, Ebusu C, Muhindo M, Arinaitwe E, Briggs J, Greenhouse B, Tappero J, Kamya M, Dorsey G, Feeney M
    PLoS Pathog, 2014;10(1):e1003864.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Autocrine IL-10 promotes human B-cell differentiation into IgM- or IgG-secreting plasmablasts.
    Authors: Heine G, Drozdenko G, Grun J, Chang H, Radbruch A, Worm M
    Eur J Immunol, 2014;44(6):1615-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. Immunomodulatory properties of a viral homolog of human interleukin-10 expressed by human cytomegalovirus during the latent phase of infection.
    Authors: Jenkins C, Garcia W, Godwin MJ, Spencer JV, Stern JL, Abendroth A, Slobedman B
    J. Virol., 2008;82(7):3736-50.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor function.
    Authors: Godfrey WR, Spoden DJ, Ge YG, Baker SR, Liu B, Levine BL, June CH, Blazar BR, Porter SB
    Blood, 2005;105(2):750-8.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Neutralization
  9. Human cytomegalovirus-encoded interleukin-10 homolog inhibits maturation of dendritic cells and alters their functionality.
    Authors: Chang WL, Baumgarth N, Yu D, Barry PA
    J. Virol., 2004;78(16):8720-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  10. In vitro-expanded human CD4(+)CD25(+) T-regulatory cells can markedly inhibit allogeneic dendritic cell-stimulated MLR cultures.
    Authors: Godfrey WR, Ge YG, Spoden DJ, Levine BL, June CH, Blazar BR, Porter SB
    Blood, 2004;104(2):453-61.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Human IL‑10 R alpha Antibody
By Anonymous on 01/21/2016
Application: Used for in vivo IL-10 neutralization. This study was published PMID:25463432 Sample Tested: Peripheral blood mononuclear cells (PBMCs) Species: Human