Human IL-15R alpha Antibody
Human IL-15R alpha Antibody Summary
Accession # NP_751951
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of IL-15R alpha in Human PBMC Monocytes by Flow Cytometry. Human PBMC were treated with LPS (100 ng/ml) and recombinant human IFN gamma (285-IF; 25 ng/ml) for 24 hours, then stained with (A) Rabbit Anti-Human IL-15R alpha Monoclonal Antibody (Catalog # MAB10900) or (B) Rabbit IgG Control (MAB1050), followed by APC-conjugated anti-Rabbit IgG secondary antibody (F0111) and Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (FAB3832P). Staining was performed using our Staining Membrane-associated Proteins protocol.
Detection of IL-15R alpha in Human PBMC Monocytes by Flow Cytometry. Human PBMC were stained with (A) Rabbit Anti-Human IL-15R alpha Monoclonal Antibody (Catalog # MAB10900) or (B) Rabbit IgG Control (MAB1050), followed by APC-conjugated anti-Rabbit IgG secondary antibody (F0111) and Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (FAB3832P). Staining was performed using our Staining Membrane-associated Proteins protocol.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-15R alpha
Interleukin 15 receptor alpha (IL-15 R alpha ) is a high affinity receptor that specifically binds IL-15 with high affinity and associates as a heterotrimer with the IL-2 receptors beta and gamma subunits to initiate signal transduction. IL-15 R alpha is expressed on a wide variety of T cells and B cells as well as non-lymphoid cells. IL‑15 R alpha is a 58-60 kDa protein that shares structural similarities to the IL-2 R alpha protein. IL-15 R alpha and IL-2 R alpha genes also share similar intron-exon organization and are closely linked on human chromosome 10p14-p15. Human IL-15 R alpha shares 45% amino acid (aa) homology with the mouse form of the receptor. Eight isoforms of IL‑15 R alpha mRNA have been identified resulting from alternative splicing events involving different exons. The exclusion of exon 2 results in an IL-15 R alpha isoform that does not bind IL-15. Human IL-15 R alpha DE3 cDNA encodes a 267 aa protein that contains a 30 aa signal sequence, a 175 aa extracellular region containing one N-linked glycosylation site, a 21 aa transmembrane domain and a 41 aa cytoplasmic tail. Signaling of IL-15 can occur in one of three ways; through the heterotrimeric complex of IL-15 R alpha, IL-2 R beta, and IL-2 R gamma c, through the heterodimeric complex of IL-2 receptors beta and gamma common, through a novel 60-65 kDa IL-15 RX subunit found on mast cells. The binding of IL-15 to IL-15 R alpha has been reported to antagonize the TNF-alpha -mediated apoptosis in fibroblasts by competing with TNF RI for TRAF2 binding.
- Anderson, D.M. et al. (1995) J. Biol. Chem. 270:29862.
- Bulfone-Paus, S. et al. (1999) FASEB 13:1575.
- Waldemann, T.A. and Y. Tagaya (1999) Ann. Rev. Immunol. 17:19.
- Dubois, S. et al. (1999) J. Biol. Chem. 274:26978.
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