Human IL-17/IL-17A Antibody

Catalog #: MAB3171R Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB3171R-100
MAB3171R-SP
MAB3171R-500
MAB3171R-01M

Save 15% on Select RUO Reagents. See Details.

Detection of Human IL‑17/IL‑17A by Western Blot.

3 Images

All IL-17/IL-17A Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-17/IL-17A Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL-17 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) IL-17A/IL-17F heterodimer and no cross-reactivity with rhIL-17F, recombinant mouse IL-17, and recombinant rat IL-17A is observed.

Source

Recombinant Monoclonal Mouse IgG₁ Clone # 41802R

Purification

Protein A or G purified from cell culture supernatant

Immunogen

E.coli-derived recombinant human IL-17
Ile20-Ala155
Accession # Q16552

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human IL‑17/IL‑17A by Western Blot. Western blot shows lysates of human CD4⁺T cells. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human IL-17/IL-17A Monoclonal Antibody (Catalog # MAB3171R) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IL-17/IL-17A at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot

View Larger

Detection of Human IL‑17/IL‑17A by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-17/IL-17A. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human IL-17/IL-17A Monoclonal Antibody (Catalog # MAB3171R) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for IL-17/IL-17A at approximately 14-19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Intracellular Staining by Flow Cytometry

Detection of IL-17/IL-17A antibody in Human PBMCs antibody by Flow Cytometry.

View Larger

Detection of IL‑17/IL‑17A in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were unstimulated (light orange filled histogram) or treated with 50 ng/mL PMA and 250 ng/mL calcium ionomycin for 16 hours, then stained with Mouse Anti-Human IL-17/IL-17A Monoclonal Antibody (Catalog # MAB3171R, dark orange filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17/IL-17A

Interleukin-17 (IL-17) is a pro-inflammatory cytokine secreted by activated T cells. It is the prototype member of the IL-17 family that also includes IL-17B, C, D, E, and F.

Long Name

Interleukin 17

Entrez Gene IDs

3605 (Human); 16171 (Mouse); 301289 (Rat); 449530 (Porcine); 481837 (Canine); 102119976 (Cynomolgus Monkey)

Alternate Names

CTLA8; CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A