Human IL‑18 BPa Antibody

Detection of IL-18 BPa by Western Blot

TNFR1-Fc-enhanced axon growth occurs independently of action potential generation & the requirement for PKC activation. (a) Bar charts of length & branch number & Sholl profiles of P0 SCG neurons cultured for 24 h with either 10 ng ml−1 NGF alone (control), NGF & 10 ng ml−1 TNFR1-Fc, NGF & 100 nM TTX or NGF, TNFR1-Fc & TTX. Mean ± s.e.m of neurite arbour data of 150 neurons per condition combined from three separate experiments of each type (***p < 0.001, statistical comparison with control). (b) Representative immunoblot probed for phospho-(Ser660) PKC, total PKC & beta -III tubulin of lysates of P0 SCG neurons that were cultured for 12 h in medium containing 10 ng ml−1 NGF before being treated for 15 min with TNFR1-Fc & 1 µM mibefradil. The bar chart plots the densitometry from four separate WBs of the ratio of pPKC to total PKC, normalized to 100% for the controls, mean ± s.e.m. (c) Representative immunoblot probed for phospho-ERK1/phospho-ERK2, total ERK1/ERK2 & beta -III tubulin of lysates of P0 SCG neurons that were cultured for 12 h in medium containing 10 ng ml−1 NGF before being treated for 15 min with 10 ng ml−1 TNFR1-Fc & 10 µM GF 109203X. The bar chart plots the densitometry from four separate WBs of the ratio of pERK1/2 to total ERK1/2, normalized to 100% for the controls (*p < 0.05 & **p < 0.01, statistical comparison with control). (d) Bar charts of length & branch point number & the Sholl profiles of P0 SCG neurons cultured for 24 h with either NGF alone (control), NGF & TNFR1-Fc, NGF & GF 109203X or NGF, TNFR1-Fc & GF 109203X. The data shown represent the mean ± s.e.m. of neurite arbour data of 150 neurons per condition combined from three separate experiments of each type (***p < 0.001, statistical comparison with control). Image collected & cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28100666), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of IL-18 BPa by Western Blot

TNFR1-Fc-enhanced axon growth occurs independently of action potential generation & the requirement for PKC activation. (a) Bar charts of length & branch number & Sholl profiles of P0 SCG neurons cultured for 24 h with either 10 ng ml−1 NGF alone (control), NGF & 10 ng ml−1 TNFR1-Fc, NGF & 100 nM TTX or NGF, TNFR1-Fc & TTX. Mean ± s.e.m of neurite arbour data of 150 neurons per condition combined from three separate experiments of each type (***p < 0.001, statistical comparison with control). (b) Representative immunoblot probed for phospho-(Ser660) PKC, total PKC & beta -III tubulin of lysates of P0 SCG neurons that were cultured for 12 h in medium containing 10 ng ml−1 NGF before being treated for 15 min with TNFR1-Fc & 1 µM mibefradil. The bar chart plots the densitometry from four separate WBs of the ratio of pPKC to total PKC, normalized to 100% for the controls, mean ± s.e.m. (c) Representative immunoblot probed for phospho-ERK1/phospho-ERK2, total ERK1/ERK2 & beta -III tubulin of lysates of P0 SCG neurons that were cultured for 12 h in medium containing 10 ng ml−1 NGF before being treated for 15 min with 10 ng ml−1 TNFR1-Fc & 10 µM GF 109203X. The bar chart plots the densitometry from four separate WBs of the ratio of pERK1/2 to total ERK1/2, normalized to 100% for the controls (*p < 0.05 & **p < 0.01, statistical comparison with control). (d) Bar charts of length & branch point number & the Sholl profiles of P0 SCG neurons cultured for 24 h with either NGF alone (control), NGF & TNFR1-Fc, NGF & GF 109203X or NGF, TNFR1-Fc & GF 109203X. The data shown represent the mean ± s.e.m. of neurite arbour data of 150 neurons per condition combined from three separate experiments of each type (***p < 0.001, statistical comparison with control). Image collected & cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28100666), licensed under a CC-BY license. Not internally tested by R&D Systems.