Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Neutralization, Intracellular Staining by Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, ELISA Development

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all IL-19 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IL-19
Leu25-Ala177 (Phe175Ser)
Accession # Q9UHD0

Specificity

Detects human IL‑19 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human IL‑19 Antibody

Cell Proliferation Induced by IL‑19 and Neutralization by Human IL‑19 Antibody.

Cell Proliferation Induced by IL‑19 and Neutralization by Human IL‑19 Antibody.

Recombinant Human IL-19 (Catalog # 1035-IL) stimulates proliferation in the BaF3 mouse pro-B cell line co-transfected with human IL-20 Ra and IL-20 R beta in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-19 (3 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1035). The ND50 is typically 1-3 µg/mL.

Applications for Human IL‑19 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample: Human peripheral blood mononuclear cells fixed with paraformaldehyde and permeabilized with saponin

Western Blot

0.1 µg/mL
Sample: Recombinant Human IL‑19 (Catalog # 1035-IL)

Neutralization

Measured by its ability to neutralize IL‑19-induced proliferation in the BaF3 mouse pro‑B cell line co-transfected with human IL‑20 R alpha and IL‑20 R beta. The Neutralization Dose (ND50) is typically 1-3 µg/mL in the presence of 3 ng/mL Recombinant Human IL‑19.

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-19

Human Interleukin 19 (IL-19) is a member of the IL-10 family of related cytokines. Its gene contains two alternate translation initiation sites, generating precursors of 215 amino acids (aa) and 177 aa, respectively. Both isoforms are processed to 17 kDa, 153 aa mature molecules. IL-19 contains seven helices and is secreted as a 35 kDa monomer. There are two potential N-linked glycosylation sites, and it is likely that the molecule is glycosylated. Mature human IL-19 shares 69% aa sequence identity with the mature mouse homologue. Although mouse IL-19 is active on human cells, human IL-19 is not active on mouse cells. IL-19 expression is limited to activated keratinocytes and monocytes. IL-19 binds a receptor complex consisting of the IL-20 receptor alpha (IL-20 R alpha, also known as IL-20 R1) and the IL-20 receptor beta (IL-20 R beta or IL-20 R2). This receptor complex is also shared by IL-20 and IL-24. Functionally, IL-19 induces IL-6 and TNF-alpha production by monocytes, and drives T-helper cell differentiation towards a Th2 response (1‑5).

References

  1. Gallagher, G. et al. (2000) Genes Immun. 1:442.
  2. Gallagher, G. et al. (2004) Int. Immunopharmacol. 4:615.
  3. Laio, Y-C. et al. (2002) J. Immunol. 169:4288.
  4. Romer, J. et al. (2003) J. Invest. Dermatol. 121:1306.
  5. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.

Long Name

Interleukin 19

Alternate Names

IL19

Entrez Gene IDs

29949 (Human); 329244 (Mouse)

Gene Symbol

IL19

UniProt

Q9UHD0

Additional IL-19 Products

Product Documents for Human IL‑19 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human IL‑19 Antibody

For research use only

Citations for Human IL‑19 Antibody

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