Human IL-22 R alpha 1 Antibody

(4 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human IL‑22 R alpha 1 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human IL-20 R beta is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human IL-22 R alpha 1
    Pro18-Thr228
    Accession # Q8N6P7
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Neutralization
    Measured by its ability to neutralize IL‑22-induced IL‑10 secretion in the COLO 205 human colorectal adenocarcinoma cell line. Marehalli, L. et al. (2004) Intl. Immunopharmacol. 4:679. The Neutralization Dose (ND50) is typically 0.2-1 µg/mL in the presence of 1 ng/mL Recombinant Human IL‑22.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
IL‑10 secretion Induced by
IL‑22 and Neutralization by Human IL‑22 R alpha 1 Antibody.
Recombinant Human IL‑22 (Catalog # 782-IL) stimulates
IL‑10 secretion in the COLO 205 human colorectal adenocarcin­oma cell line in a dose-dependent manner (orange line), as measured by the Human IL‑10 DuoSet ELISA Development Kit (Catalog # DY217B). IL‑10 secretion elicited by Recombinant Human IL‑22 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑22 R alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2770). The ND50 is typically
0.2‑1 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-22 R alpha 1

IL-22 receptor, also known as IL-22 R alpha 1 and CRF2-9, is an approximately 65 kDa transmembrane glycoprotein in the type II cytokine receptor family (CRF). IL-22 R alpha 1 contains a 211 amino acid (aa) extracellular domain (ECD) with two fibronectin type III repeats, and a 323 aa cytoplasmic domain. IL-22 R alpha 1 associates with either IL-10 R beta or IL-20 R beta to form receptor complexes with distinct ligand selectivities. IL-10 R beta is a shared subunit of the IL-10, -22, -26, -28, and -29 receptors, while IL-20 R beta is a shared subunit of the IL-19, -20, -22R and -24 receptors (1). IL-22 R alpha 1/IL-10 R beta  is an IL-22 responsive receptor (2, 3), and IL-22 R alpha 1/IL-20 R beta is an IL-20 or IL-24 responsive receptor (4, 5). IL-22 R alpha 1 contains cytoplasmic motifs for interactions with signal transduction molecules, but formation of ternary complexes with IL-10 R beta or IL-20 R beta and the respective ligands is required for signal transduction (2, 6). IL-22BP functions as a competitive antagonist by binding

IL‑22 and preventing its association with IL-22 R alpha 1 (7, 9). Even though it is a receptor for interleukins, IL-22 R alpha 1 is not expressed on hematopoietic cells (6, 10, 11). Instead, IL-22 R alpha 1 expression is restricted to epithelial and stromal cells (6, 10‑13). IL-22 R alpha 1 signaling promotes innate immune responses and wound healing at sites of infection and inflammation. This includes upregulation of antimicrobial, acute phase, proinflammatory, and extracellular matrix proteins as well as proteases (3, 11, 13, 14). IL-22 R alpha 1 signaling also promotes downregulation of proteins involved in keratinocyte differentiation (3, 14). Within the ECD, human IL-22 R alpha 1 shares 78%, 76%, and 83% aa sequence identity with mouse, rat, and canine IL-22 R, respectively. It shares 22% - 25% aa sequence identity with the ECDs of other class II receptors IL-10 R, IL-20 R, and IL-28 R.

  • References:
    1. Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33.
    2. Xie, M.-H. et al. (2000) J. Biol. Chem. 275:31335.
    3. Boniface, K. et al. (2005) J. Immunol. 174:3695.
    4. Dumoutier, L. et al. (2001) J. Immunol. 167:3545.
    5. Wang, M. et al. (2002) J. Biol. Chem. 277:7341.
    6. Kotenko, S.V. et al. (2001) J. Biol. Chem. 276:2725.
    7. Li, J. et al. (2004) Int. Immunopharmacol. 4:693.
    8. Logsdon, N.J. et al. (2002) J. Interferon Cytokine Res. 22:1099.
    9. Kotenko, S.V. et al. (2001) J. Immunol. 166:7096.
    10. Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:577.
    11. Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:679.
    12. Aggarwal, S. et al. (2001) J. Interferon Cytokine Res. 21:1047.
    13. Wolk, K. et al. (2004) Immunity 21:241.
    14. Wolk, K. et al. (2006) Eur. J. Immunol. 36:1309.
  • Long Name:
    Interleukin 22 Receptor
  • Entrez Gene IDs:
    58985 (Human); 230828 (Mouse)
  • Alternate Names:
    CRF2-9; CRF2-9interleukin 22 receptor; Cytokine receptor class-II member 9; Cytokine receptor family 2 member 9; IL-22 R alpha 1; IL-22 receptor subunit alpha-1; IL22R alpha 1; IL22R; IL22R1; IL22RA1; IL-22Ra1; IL-22R-alpha-1; IL-TIF-R1; interleukin 22 receptor, alpha 1; interleukin-22 receptor subunit alpha-1; zcytoR11
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. IL-22 negatively regulates Helicobacter pylori-induced CCL20 expression in gastric epithelial cells.
    Authors: Chen J, Wu M, Kuo S, Liao F
    PLoS ONE, 2014;9(5):e97350.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  2. IL-22 contributes to TGF-beta1-mediated epithelial-mesenchymal transition in asthmatic bronchial epithelial cells.
    Authors: Johnson J, Nishioka M, Chakir J, Risse P, Almaghlouth I, Bazarbashi A, Plante S, Martin J, Eidelman D, Hamid Q
    Respir Res, 2013;14(0):118.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. IL-22 suppresses IFN-gamma-mediated lung inflammation in asthmatic patients.
    Authors: Pennino D, Bhavsar P, Effner R, Avitabile S, Venn P, Quaranta M, Marzaioli V, Cifuentes L, Durham S, Cavani A, Eyerich K, Chung K, Schmidt-Weber C, Eyerich S
    J Allergy Clin Immunol, 2013;131(2):562-70.
    Species: Human
    Sample Type: Whole Cells
    Application: Blocking
  4. IL-17 and IL-22 mediate IL-20 subfamily cytokine production in cultured keratinocytes via increased IL-22 receptor expression.
    Authors: Tohyama M, Hanakawa Y, Shirakata Y, Dai X, Yang L, Hirakawa S, Tokumaru S, Okazaki H, Sayama K, Hashimoto K
    Eur. J. Immunol., 2009;39(10):2779-88.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
Isotype Controls
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Normal Goat IgG Control

Ctrl AB-108-C 191  
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