Human IL-22 R alpha 1 Antibody

Catalog # Availability Size / Price Qty
AF2770
AF2770-SP
IL‑10 secretion Induced by IL‑22 and Neutralization by Human IL‑22 R alpha 1 Antibody.
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Product Details
Citations (5)
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Human IL-22 R alpha 1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL‑22 R alpha 1 in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human IL-20 R beta is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human IL-22 R alpha 1
Pro18-Thr228
Accession # Q8N6P7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Neutralization
Measured by its ability to neutralize IL‑22-induced IL‑10 secretion in the COLO 205 human colorectal adenocarcinoma cell line. Marehalli, L. et al. (2004) Intl. Immunopharmacol. 4:679. The Neutralization Dose (ND50) is typically 0.2-1 µg/mL in the presence of 1 ng/mL Recombinant Human IL‑22.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization IL‑10 secretion Induced by IL‑22 and Neutralization by Human IL‑22 R alpha 1 Antibody. View Larger

IL‑10 secretion Induced by IL‑22 and Neutralization by Human IL‑22 R alpha 1 Antibody. Recombinant Human IL-22 (Catalog # 782-IL) stimulates IL-10 secretion in the COLO 205 human colorectal adenocarcin-oma cell line in a dose-dependent manner (orange line), as measured by the Human IL-10 DuoSet ELISA Development Kit (Catalog # DY217B). IL-10 secretion elicited by Recombinant Human IL-22 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-22 Ra1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2770). The ND50 is typically 0.2-1 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-22 R alpha 1

IL-22 receptor, also known as IL-22 R alpha 1 and CRF2-9, is an approximately 65 kDa transmembrane glycoprotein in the type II cytokine receptor family (CRF). IL-22 R alpha 1 contains a 211 amino acid (aa) extracellular domain (ECD) with two fibronectin type III repeats, and a 323 aa cytoplasmic domain. IL-22 R alpha 1 associates with either IL-10 R beta or IL-20 R beta to form receptor complexes with distinct ligand selectivities. IL-10 R beta is a shared subunit of the IL-10, -22, -26, -28, and -29 receptors, while IL-20 R beta is a shared subunit of the IL-19, -20, -22R and -24 receptors (1). IL-22 R alpha 1/IL-10 R beta  is an IL-22 responsive receptor (2, 3), and IL-22 R alpha 1/IL-20 R beta is an IL-20 or IL-24 responsive receptor (4, 5). IL-22 R alpha 1 contains cytoplasmic motifs for interactions with signal transduction molecules, but formation of ternary complexes with IL-10 R beta or IL-20 R beta and the respective ligands is required for signal transduction (2, 6). IL-22BP functions as a competitive antagonist by binding

IL‑22 and preventing its association with IL-22 R alpha 1 (7, 9). Even though it is a receptor for interleukins, IL-22 R alpha 1 is not expressed on hematopoietic cells (6, 10, 11). Instead, IL-22 R alpha 1 expression is restricted to epithelial and stromal cells (6, 10‑13). IL-22 R alpha 1 signaling promotes innate immune responses and wound healing at sites of infection and inflammation. This includes upregulation of antimicrobial, acute phase, proinflammatory, and extracellular matrix proteins as well as proteases (3, 11, 13, 14). IL-22 R alpha 1 signaling also promotes downregulation of proteins involved in keratinocyte differentiation (3, 14). Within the ECD, human IL-22 R alpha 1 shares 78%, 76%, and 83% aa sequence identity with mouse, rat, and canine IL-22 R, respectively. It shares 22% - 25% aa sequence identity with the ECDs of other class II receptors IL-10 R, IL-20 R, and IL-28 R.

References
  1. Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33.
  2. Xie, M.-H. et al. (2000) J. Biol. Chem. 275:31335.
  3. Boniface, K. et al. (2005) J. Immunol. 174:3695.
  4. Dumoutier, L. et al. (2001) J. Immunol. 167:3545.
  5. Wang, M. et al. (2002) J. Biol. Chem. 277:7341.
  6. Kotenko, S.V. et al. (2001) J. Biol. Chem. 276:2725.
  7. Li, J. et al. (2004) Int. Immunopharmacol. 4:693.
  8. Logsdon, N.J. et al. (2002) J. Interferon Cytokine Res. 22:1099.
  9. Kotenko, S.V. et al. (2001) J. Immunol. 166:7096.
  10. Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:577.
  11. Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:679.
  12. Aggarwal, S. et al. (2001) J. Interferon Cytokine Res. 21:1047.
  13. Wolk, K. et al. (2004) Immunity 21:241.
  14. Wolk, K. et al. (2006) Eur. J. Immunol. 36:1309.
Long Name
Interleukin 22 Receptor
Entrez Gene IDs
58985 (Human); 230828 (Mouse)
Alternate Names
CRF2-9; CRF2-9interleukin 22 receptor; Cytokine receptor class-II member 9; Cytokine receptor family 2 member 9; IL-22 R alpha 1; IL-22 receptor subunit alpha-1; IL22R alpha 1; IL22R; IL22R1; IL22RA1; IL-22Ra1; IL-22R-alpha-1; IL-TIF-R1; interleukin 22 receptor, alpha 1; interleukin-22 receptor subunit alpha-1; zcytoR11

Product Datasheets

Citations for Human IL-22 R alpha 1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Interleukin-22 regulates B3GNT7 expression to induce fucosylation of glycoproteins in intestinal epithelial cells
    Authors: DJ Carroll, MWN Burns, L Mottram, DC Propheter, A Boucher, GM Lessen, A Kumar, SA Malaker, C Xing, LV Hooper, U Yrlid, JJ Kohler
    The Journal of Biological Chemistry, 2021;0(0):101463.
    Species: Human
    Sample Types: Organoid
    Applications: Neutralization
  2. IL-22 negatively regulates Helicobacter pylori-induced CCL20 expression in gastric epithelial cells.
    Authors: Chen J, Wu M, Kuo S, Liao F
    PLoS ONE, 2014;9(5):e97350.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. IL-22 contributes to TGF-beta1-mediated epithelial-mesenchymal transition in asthmatic bronchial epithelial cells.
    Authors: Johnson J, Nishioka M, Chakir J, Risse P, Almaghlouth I, Bazarbashi A, Plante S, Martin J, Eidelman D, Hamid Q
    Respir Res, 2013;14(0):118.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. IL-22 suppresses IFN-gamma-mediated lung inflammation in asthmatic patients.
    Authors: Pennino D, Bhavsar P, Effner R, Avitabile S, Venn P, Quaranta M, Marzaioli V, Cifuentes L, Durham S, Cavani A, Eyerich K, Chung K, Schmidt-Weber C, Eyerich S
    J Allergy Clin Immunol, 2013;131(2):562-70.
    Species: Human
    Sample Types: Whole Cells
    Applications: Blocking
  5. IL-17 and IL-22 mediate IL-20 subfamily cytokine production in cultured keratinocytes via increased IL-22 receptor expression.
    Authors: Tohyama M, Hanakawa Y, Shirakata Y, Dai X, Yang L, Hirakawa S, Tokumaru S, Okazaki H, Sayama K, Hashimoto K
    Eur. J. Immunol., 2009;39(10):2779-88.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Human IL-22 R alpha 1 Antibody
By Anonymous on 06/09/2020
Application: ELISA Sample Tested: Serum and Plasma Species: Human

We used this antibody in an in-house ELISA along with mAb (2770) and protein standard (2770-LR) to quantify IL-22R alpha1 in human serum and plasma. This combination could not detect IL-22R alpha1 in our samples but generated a good standard curve.