Human IL-22 R alpha 1 Antibody
Human IL-22 R alpha 1 Antibody Summary
Pro18-Thr228
Accession # Q8N6P7
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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IL‑10 secretion Induced by IL‑22 and Neutralization by Human IL‑22 R alpha 1 Antibody. Recombinant Human IL-22 (Catalog # 782-IL) stimulates IL-10 secretion in the COLO 205 human colorectal adenocarcin-oma cell line in a dose-dependent manner (orange line), as measured by the Human IL-10 DuoSet ELISA Development Kit (Catalog # DY217B). IL-10 secretion elicited by Recombinant Human IL-22 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-22 Ra1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2770). The ND50 is typically 0.2-1 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-22 R alpha 1
IL-22 receptor, also known as IL-22 R alpha 1 and CRF2-9, is an approximately 65 kDa transmembrane glycoprotein in the type II cytokine receptor family (CRF). IL-22 R alpha 1 contains a 211 amino acid (aa) extracellular domain (ECD) with two fibronectin type III repeats, and a 323 aa cytoplasmic domain. IL-22 R alpha 1 associates with either IL-10 R beta or IL-20 R beta to form receptor complexes with distinct ligand selectivities. IL-10 R beta is a shared subunit of the IL-10, -22, -26, -28, and -29 receptors, while IL-20 R beta is a shared subunit of the IL-19, -20, -22R and -24 receptors (1). IL-22 R alpha 1/IL-10 R beta is an IL-22 responsive receptor (2, 3), and IL-22 R alpha 1/IL-20 R beta is an IL-20 or IL-24 responsive receptor (4, 5). IL-22 R alpha 1 contains cytoplasmic motifs for interactions with signal transduction molecules, but formation of ternary complexes with IL-10 R beta or IL-20 R beta and the respective ligands is required for signal transduction (2, 6). IL-22BP functions as a competitive antagonist by binding
IL‑22 and preventing its association with IL-22 R alpha 1 (7, 9). Even though it is a receptor for interleukins, IL-22 R alpha 1 is not expressed on hematopoietic cells (6, 10, 11). Instead, IL-22 R alpha 1 expression is restricted to epithelial and stromal cells (6, 10‑13). IL-22 R alpha 1 signaling promotes innate immune responses and wound healing at sites of infection and inflammation. This includes upregulation of antimicrobial, acute phase, proinflammatory, and extracellular matrix proteins as well as proteases (3, 11, 13, 14). IL-22 R alpha 1 signaling also promotes downregulation of proteins involved in keratinocyte differentiation (3, 14). Within the ECD, human IL-22 R alpha 1 shares 78%, 76%, and 83% aa sequence identity with mouse, rat, and canine IL-22 R, respectively. It shares 22% - 25% aa sequence identity with the ECDs of other class II receptors IL-10 R, IL-20 R, and IL-28 R.
- Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33.
- Xie, M.-H. et al. (2000) J. Biol. Chem. 275:31335.
- Boniface, K. et al. (2005) J. Immunol. 174:3695.
- Dumoutier, L. et al. (2001) J. Immunol. 167:3545.
- Wang, M. et al. (2002) J. Biol. Chem. 277:7341.
- Kotenko, S.V. et al. (2001) J. Biol. Chem. 276:2725.
- Li, J. et al. (2004) Int. Immunopharmacol. 4:693.
- Logsdon, N.J. et al. (2002) J. Interferon Cytokine Res. 22:1099.
- Kotenko, S.V. et al. (2001) J. Immunol. 166:7096.
- Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:577.
- Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:679.
- Aggarwal, S. et al. (2001) J. Interferon Cytokine Res. 21:1047.
- Wolk, K. et al. (2004) Immunity 21:241.
- Wolk, K. et al. (2006) Eur. J. Immunol. 36:1309.
Product Datasheets
Citations for Human IL-22 R alpha 1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Interleukin-22 regulates B3GNT7 expression to induce fucosylation of glycoproteins in intestinal epithelial cells
Authors: DJ Carroll, MWN Burns, L Mottram, DC Propheter, A Boucher, GM Lessen, A Kumar, SA Malaker, C Xing, LV Hooper, U Yrlid, JJ Kohler
The Journal of Biological Chemistry, 2021-12-02;0(0):101463.
Species: Human
Sample Types: Organoid
Applications: Neutralization -
IL-22 negatively regulates Helicobacter pylori-induced CCL20 expression in gastric epithelial cells.
Authors: Chen J, Wu M, Kuo S, Liao F
PLoS ONE, 2014-05-13;9(5):e97350.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
IL-22 contributes to TGF-beta1-mediated epithelial-mesenchymal transition in asthmatic bronchial epithelial cells.
Authors: Johnson J, Nishioka M, Chakir J, Risse P, Almaghlouth I, Bazarbashi A, Plante S, Martin J, Eidelman D, Hamid Q
Respir Res, 2013-11-01;14(0):118.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
IL-22 suppresses IFN-gamma-mediated lung inflammation in asthmatic patients.
Authors: Pennino D, Bhavsar P, Effner R, Avitabile S, Venn P, Quaranta M, Marzaioli V, Cifuentes L, Durham S, Cavani A, Eyerich K, Chung K, Schmidt-Weber C, Eyerich S
J Allergy Clin Immunol, 2012-11-19;131(2):562-70.
Species: Human
Sample Types: Whole Cells
Applications: Blocking -
IL-17 and IL-22 mediate IL-20 subfamily cytokine production in cultured keratinocytes via increased IL-22 receptor expression.
Authors: Tohyama M, Hanakawa Y, Shirakata Y, Dai X, Yang L, Hirakawa S, Tokumaru S, Okazaki H, Sayama K, Hashimoto K
Eur. J. Immunol., 2009-10-01;39(10):2779-88.
Species: Human
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC-Fr, Western Blot
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We used this antibody in an in-house ELISA along with mAb (2770) and protein standard (2770-LR) to quantify IL-22R alpha1 in human serum and plasma. This combination could not detect IL-22R alpha1 in our samples but generated a good standard curve.
