Human IL-36 beta /IL-1F8 Antibody

(2 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human IL‑36 beta /IL‑1F8 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human
    (rh) IL-36 gamma and rhIL-36 alpha is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human IL‑36 beta /IL‑1F8
    Met1-Glu157
    Accession # Q3MIH0
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human IL‑36 beta /IL‑1F8 (Catalog # 1099-IL)
  • Immunohistochemistry
    5-15 µg/mL
    Immersion fixed paraffin-embedded sections of human tonsil subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
  • Blockade of Receptor-ligand Interaction
    In a functional ELISA, 1.5-6 µg/mL of this antibody will block 50% of the binding of 5 μg/mL of Recombinant Human IL-1 Rrp2/IL-1 R6 Fc Chimera (Catalog # 872-RP) to immobilized Recombinant Human IL‑36 beta /IL‑1F8 (Catalog # 1099-IL) coated at 1 µg/mL (100 µL/well). At 100 μg/mL, this antibody will block >90% of the binding.
  • Neutralization
    Measured by its ability to neutralize IL‑36 beta /IL‑1F8-induced IL-8 secretion in human pre-adipocytes. van Asseldonk, E.J. et al. (2010) Obesity 18:2234.The Neutralization Dose (ND50) is typically 50-300 ng/mL in the presence of 20 ng/mL Recombinant Human IL‑36 beta /IL‑1F8 (aa 5-157).
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell IL-8 Secretion Induced by IL‑36 beta /IL‑1F8 and Neutral­ization by Human IL‑36 beta / IL‑1F8 Antibody.

Recombinant Human IL‑36 beta /
IL‑1F8 (aa 5-157) (Catalog # 6834-IL) induces IL-8 secretion in human pre-adipocytes in a dose-dependent manner (orange line). IL-8 Secretion elicited by Recombinant Human IL‑36 beta /
IL‑1F8 (aa 5-157) (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑36 beta /IL‑1F8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1099). The ND50 is typically 50‑300 ng/mL.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-36 beta/IL-1F8

Human interleukin 1 family member #8 [IL-1F8; also named Interleukin-36 beta, IL36B, FIL-1 eta  (eta) and IL-1H2] is a member of the IL-1 family of proteins (1‑3). IL-1 family members include IL‑1 beta, IL-1 alpha, IL-1ra, IL-18 and IL-1F5 through F10 (4). All family members show a 12 beta -stranded beta -trefoil configuration, and are believed to have arisen from a common ancestral gene that has undergone multiple duplications (4). Two alternatively spliced transcript variants encode distinct (164 or 157 residues) protein isoforms that differ in their C-terminal 70 amino acid (aa) residues have been reported (3). IL-1F8 isoform 2 is synthesized as a 157 aa protein that contains no signal sequence and no prosegment (1, 2). Unlike IL-1F8 isoform 1 which lacks potential N‑linked glycosylation sites, isoform 2 contains one potential N-linked glycosylation site in its unique C-terminus. IL-1F8 is reported to be actively secreted (1). Human IL-1F8 isoform 2 shares 61% aa identity with mouse IL-1 ra, a 183 aa form of IL‑1F8. Within the IL-1 family, IL-1F8 shares 30%, 32%, 37%, 46%, 34%, 45% and 28% aa sequence identity with IL-1 ra, IL-1 beta, IL-1F5, F6, F7, F9 and F10, respectively. Cells reported to express IL-1F8 include resting and activated monocytes and B cells (1, 4). The receptor for IL-1F8 is reported to be a combination of IL-1 Rrp2 and IL-1 RAcP (5). Recombinant IL-1F8, along with IL-1F6 and IL-1F9, has been shown to activate the pathway involving NF-kappa B and MAPK in an IL-1 Rrp2 dependent manner.

  • References:
    1. Smith, D.E. et al. (2000) J. Biol. Chem. 275:1169.
    2. Kumar, S. et al. (2000) J. Biol. Chem. 275:10308.
    3. Nicklin, M.J.H. et al. (2002) Genomics 79:718.
    4. Dunn, E. et al. (2001) Trends Immunol. 22:533.
    5. Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.
  • Long Name:
    Interleukin 36 beta/Interleukin 1 Family 8
  • Entrez Gene IDs:
    27177 (Human); 69677 (Mouse)
  • Alternate Names:
    family of interleukin 1-eta; FIL1 eta; FIL1; FIL1-(ETA); FIL1H; FILI-(ETA); IL-1 eta; IL1-ETA; IL1F8 (Canonical product IL-1F8a); IL-1F8 (FIL1-eta); IL1F8; IL-1F8; IL1H2; IL-1H2; IL36 beta; IL-36 beta; IL36B; interleukin 1 family, member 8 (eta); interleukin 1, eta; Interleukin 36, Beta; Interleukin-1 eta; interleukin-1 family member 8; Interleukin-1 homolog 2; Interleukin-1 Superfamily e; Interleukin-36 Beta
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. The double-stranded RNA analogue polyinosinic-polycytidylic acid induces keratinocyte pyroptosis and release of IL-36 gamma.
    Authors: Lian LH, Milora KA, Manupipatpong KK, Jensen LE
    J. Invest. Dermatol., 2012;132(5):1346-53.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. The new IL-1 family member IL-1F8 stimulates production of inflammatory mediators by synovial fibroblasts and articular chondrocytes.
    Authors: Magne D, Palmer G, Barton JL, Mezin F, Talabot-Ayer D, Bas S, Duffy T, Noger M, Guerne PA, Nicklin MJ, Gabay C
    Arthritis Res. Ther., 2006;8(3):R80.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
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