Human IL-4I1 Antibody

Catalog # Availability Size / Price Qty
MAB5684-100
MAB5684-SP
Detection of Human IL‑4I1 by Western Blot.
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Human IL-4I1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-4I1 in direct ELISAs.
Source
Monoclonal Rat IgG2B Clone # 1006202
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese Hamster Ovary cell line, CHO-derived human IL-4I1
Met1-His567
Accession # Q96RQ9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below
Immunocytochemistry
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human IL-4I1 antibody by Western Blot. View Larger

Detection of Human IL‑4I1 by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA for 24 hours and 10 µg/mL LPS for 3 hours. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human IL-4I1 Monoclonal Antibody (Catalog # MAB5684) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for IL-4I1 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry IL-4I1 antibody in HDLM-2 Human Cell Line by Immunocytochemistry (ICC). View Larger

IL‑4I1 in HDLM‑2 Human Cell Line. IL-4I1 was detected in immersion fixed HDLM-2 human Hodgkin's lymphoma cell line using Rat Anti-Human IL-4I1 Monoclonal Antibody (Catalog # MAB5684) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm in lysosomes. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry IL-4I1 antibody in Human B Cell Lymphoma by Immunohistochemistry (IHC-P). View Larger

IL‑4I1 in Human B Cell Lymphoma. IL-4I1 was detected in immersion fixed paraffin-embedded sections of human B cell lymphoma using Rat Anti-Human IL-4I1 Monoclonal Antibody (Catalog # MAB5684) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-4I1

Interleukin 4 induced protein 1 (IL-4I1), also known as protein FIG-1 and L-amino acid oxidase, is encoded by a B-cell IL-4-inducible gene, FIG1, and is highly expressed in primary metastinal B-cell lymphomas (1-4). It belongs to the flavin monoamine oxidase family, FIG1 subfamily. Enzymological characterization reveals that IL-4I1 has L-amino acid oxidase activity with preference toward aromatic amino acids. Studies have shown that hIL-4I1 inhibited the proliferation of CD3‑stimulated T lymphocytes with a similar effect on CD4(+) and CD8(+) T cells (5). Its inhibitory effect was dependent on enzymatic activity and H2O2 production. Its restricted expression to lymphoid tissues indicates that it may play an important function in the immune system (1, 4).

References
  1. Chu, C.C. and W.E. Paul. (1997) Proc. Natl. Acad. Sci. USA 94:2507.
  2. Mason, J.M. et al. (2004) J. Immunol. 173:4561.
  3. Chavan, S.S. et al. (2002) Biochim. Biophys. Acta. 1576:70.
  4. Copie-Bergman, C. et al. (2003) Blood 101:2756.
  5. Boulland, M.L. et al. (2007) Blood 110:220.
Long Name
Interleukin 4-induced Protein-1
Entrez Gene IDs
259307 (Human); 14204 (Mouse); 308575 (Rat); 102115096 (Cynomolgus Monkey)
Alternate Names
Fig-1 protein; FIG1; hFIG1; IL4I1; IL-4I1; IL4-induced protein 1; interleukin 4 induced 1; interleukin four induced 1; LAAO; L-amino-acid oxidase; LAO; UNQ636/PRO1265

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Human IL-4I1 Antibody
By Anonymous on 07/25/2022
Application: Immunocytochemistry/Immunofluorescence Sample Tested: HDLM-2 human Hodgkin's lymphoma cell line Species: Human