Human IL‑4I1 Antibody

Detection of IL-4I1 by Western Blot

IFN-gamma and TNF-alpha increase the expression of IL4I1 in MuSCs through NF-kappa B and STAT6 pathway.A Volcano plot of differentially expressed genes in MuSCs after the stimulation of IFN-gamma and TNF-alpha (10 ng/ml each) for 24 h using RNA-seq. B Volcano plot of differentially expressed genes in MSCs after the stimulation of IFN-gamma and TNF-alpha (10 ng/ml each) for 24 h using RNA-seq. C The mRNA expression of IL4I1 in MSCs and MuSCs after the stimulation of IFN-gamma and TNF-alpha (10 ng/ml each) for 24 h was assayed by qRT-PCR. D The concentration of IL4I1 in the supernatants of MuSCs after the stimulation of IFN-gamma and TNF-alpha (10 ng/ml each) for 24 h was measured by ELISA. E The protein expression of IL4I1 and beta -ACTIN (loading control) in MuSCs after the stimulation of IFN-gamma and TNF-alpha (10 ng/ml each) for 24 h was determined by western blotting. F The mRNA and protein expression levels of IL4I1 in MuSCs stimulated with IFN-gamma and TNF-alpha (10 ng/ml each) for 24 h in the presence or absence of AS1517499 were respectively assayed by qRT-PCR and western blotting. G The protein expression levels of IL4I1 in MuSCs stimulated with IFN-gamma and TNF-alpha (10 ng/ml each) for 24 h in the presence or absence of BAY117082 were assayed by western blotting. Data were shown as means ± SEM. Data were representative of three experiments with similar results. For two-group comparison, statistical analysis was performed by Student’s t test. ***P < 0.001; ****P < 0.0001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37507432), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of IL-4I1 by Western Blot

IL4I1 mediates the therapeutic effect of MuSCs on ALI.A The therapeutic strategy of MuSCs in the LPS-induced ALI model. Mice were treated with 2 mg/kg LPS through endotracheal infusion. 1 h later, Ctrl-shRNA MuSCs or IL4I1-shRNA MuSCs (5 × 105) pretreated with IFN-gamma and TNF-alpha (10 ng/ml each) for 24 h were intravenously injected into mice. Then, all experimental mice were euthanized after 23 h, and the lung samples were collected for further processing. B The efficiency of IL4I1 knockdown measured by western blotting analysis. C The total amount of IL4I1 protein in the lung tissue homogenates of ALI mice was determined by ELISA (PBS: n = 3, LPS: n = 3, LPS+Ctrl-shRNA MuSCs: n = 3, LPS + IL4I1-shRNA MuSCs: n = 3). D The expression levels of IL-6 mRNA (left) and protein (right) in the lung tissue homogenates of ALI mice were respectively determined by qRT-PCR and ELISA. E Lung tissues of mice with various treatments were fixed for H&E staining. Yellow arrowhead, area of widespread septal thickening with increased air-space cellularity and exudation and enhanced interstitial immune cell infiltration in the damaged lungs of ALI mice. Scale bars, 250 μm. F The expression levels of chemokines in the lung tissue homogenates of ALI mice were determined by qRT-PCR. G Lung tissues of mice with various treatments were stained with CXCL1 antibody. Scale bars, 250 μm (PBS: n = 3, LPS: n = 4, LPS+Ctrl-shRNA MuSCs: n = 5, LPS + IL4I1-shRNA MuSCs: n = 5). Data were shown as means ± SEM. Data were representative of three experiments with similar results. For multiple group comparison, statistical analysis was performed by one-way ANOVA test. *P < 0.05; **P < 0.01; ***P < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37507432), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of IL-4I1 by Western Blot

IFN-gamma and TNF-alpha increase the expression of IL4I1 in MuSCs through NF-kappa B and STAT6 pathway.A Volcano plot of differentially expressed genes in MuSCs after the stimulation of IFN-gamma and TNF-alpha (10 ng/ml each) for 24 h using RNA-seq. B Volcano plot of differentially expressed genes in MSCs after the stimulation of IFN-gamma and TNF-alpha (10 ng/ml each) for 24 h using RNA-seq. C The mRNA expression of IL4I1 in MSCs and MuSCs after the stimulation of IFN-gamma and TNF-alpha (10 ng/ml each) for 24 h was assayed by qRT-PCR. D The concentration of IL4I1 in the supernatants of MuSCs after the stimulation of IFN-gamma and TNF-alpha (10 ng/ml each) for 24 h was measured by ELISA. E The protein expression of IL4I1 and beta -ACTIN (loading control) in MuSCs after the stimulation of IFN-gamma and TNF-alpha (10 ng/ml each) for 24 h was determined by western blotting. F The mRNA and protein expression levels of IL4I1 in MuSCs stimulated with IFN-gamma and TNF-alpha (10 ng/ml each) for 24 h in the presence or absence of AS1517499 were respectively assayed by qRT-PCR and western blotting. G The protein expression levels of IL4I1 in MuSCs stimulated with IFN-gamma and TNF-alpha (10 ng/ml each) for 24 h in the presence or absence of BAY117082 were assayed by western blotting. Data were shown as means ± SEM. Data were representative of three experiments with similar results. For two-group comparison, statistical analysis was performed by Student’s t test. ***P < 0.001; ****P < 0.0001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37507432), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of IL-4I1 by Western Blot

IFN-gamma and TNF-alpha increase the expression of IL4I1 in MuSCs through NF-kappa B and STAT6 pathway.A Volcano plot of differentially expressed genes in MuSCs after the stimulation of IFN-gamma and TNF-alpha (10 ng/ml each) for 24 h using RNA-seq. B Volcano plot of differentially expressed genes in MSCs after the stimulation of IFN-gamma and TNF-alpha (10 ng/ml each) for 24 h using RNA-seq. C The mRNA expression of IL4I1 in MSCs and MuSCs after the stimulation of IFN-gamma and TNF-alpha (10 ng/ml each) for 24 h was assayed by qRT-PCR. D The concentration of IL4I1 in the supernatants of MuSCs after the stimulation of IFN-gamma and TNF-alpha (10 ng/ml each) for 24 h was measured by ELISA. E The protein expression of IL4I1 and beta -ACTIN (loading control) in MuSCs after the stimulation of IFN-gamma and TNF-alpha (10 ng/ml each) for 24 h was determined by western blotting. F The mRNA and protein expression levels of IL4I1 in MuSCs stimulated with IFN-gamma and TNF-alpha (10 ng/ml each) for 24 h in the presence or absence of AS1517499 were respectively assayed by qRT-PCR and western blotting. G The protein expression levels of IL4I1 in MuSCs stimulated with IFN-gamma and TNF-alpha (10 ng/ml each) for 24 h in the presence or absence of BAY117082 were assayed by western blotting. Data were shown as means ± SEM. Data were representative of three experiments with similar results. For two-group comparison, statistical analysis was performed by Student’s t test. ***P < 0.001; ****P < 0.0001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37507432), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of IL-4I1 by Western Blot

IL4I1 mediates the therapeutic effect of MuSCs on ALI.A The therapeutic strategy of MuSCs in the LPS-induced ALI model. Mice were treated with 2 mg/kg LPS through endotracheal infusion. 1 h later, Ctrl-shRNA MuSCs or IL4I1-shRNA MuSCs (5 × 105) pretreated with IFN-gamma and TNF-alpha (10 ng/ml each) for 24 h were intravenously injected into mice. Then, all experimental mice were euthanized after 23 h, and the lung samples were collected for further processing. B The efficiency of IL4I1 knockdown measured by western blotting analysis. C The total amount of IL4I1 protein in the lung tissue homogenates of ALI mice was determined by ELISA (PBS: n = 3, LPS: n = 3, LPS+Ctrl-shRNA MuSCs: n = 3, LPS + IL4I1-shRNA MuSCs: n = 3). D The expression levels of IL-6 mRNA (left) and protein (right) in the lung tissue homogenates of ALI mice were respectively determined by qRT-PCR and ELISA. E Lung tissues of mice with various treatments were fixed for H&E staining. Yellow arrowhead, area of widespread septal thickening with increased air-space cellularity and exudation and enhanced interstitial immune cell infiltration in the damaged lungs of ALI mice. Scale bars, 250 μm. F The expression levels of chemokines in the lung tissue homogenates of ALI mice were determined by qRT-PCR. G Lung tissues of mice with various treatments were stained with CXCL1 antibody. Scale bars, 250 μm (PBS: n = 3, LPS: n = 4, LPS+Ctrl-shRNA MuSCs: n = 5, LPS + IL4I1-shRNA MuSCs: n = 5). Data were shown as means ± SEM. Data were representative of three experiments with similar results. For multiple group comparison, statistical analysis was performed by one-way ANOVA test. *P < 0.05; **P < 0.01; ***P < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37507432), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of IL-4I1 by Western Blot

IFN-gamma and TNF-alpha increase the expression of IL4I1 in MuSCs through NF-kappa B and STAT6 pathway.A Volcano plot of differentially expressed genes in MuSCs after the stimulation of IFN-gamma and TNF-alpha (10 ng/ml each) for 24 h using RNA-seq. B Volcano plot of differentially expressed genes in MSCs after the stimulation of IFN-gamma and TNF-alpha (10 ng/ml each) for 24 h using RNA-seq. C The mRNA expression of IL4I1 in MSCs and MuSCs after the stimulation of IFN-gamma and TNF-alpha (10 ng/ml each) for 24 h was assayed by qRT-PCR. D The concentration of IL4I1 in the supernatants of MuSCs after the stimulation of IFN-gamma and TNF-alpha (10 ng/ml each) for 24 h was measured by ELISA. E The protein expression of IL4I1 and beta -ACTIN (loading control) in MuSCs after the stimulation of IFN-gamma and TNF-alpha (10 ng/ml each) for 24 h was determined by western blotting. F The mRNA and protein expression levels of IL4I1 in MuSCs stimulated with IFN-gamma and TNF-alpha (10 ng/ml each) for 24 h in the presence or absence of AS1517499 were respectively assayed by qRT-PCR and western blotting. G The protein expression levels of IL4I1 in MuSCs stimulated with IFN-gamma and TNF-alpha (10 ng/ml each) for 24 h in the presence or absence of BAY117082 were assayed by western blotting. Data were shown as means ± SEM. Data were representative of three experiments with similar results. For two-group comparison, statistical analysis was performed by Student’s t test. ***P < 0.001; ****P < 0.0001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37507432), licensed under a CC-BY license. Not internally tested by R&D Systems.