Human IL-6 R alpha Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB2271R-100
MAB2271R-SP
MAB2271R-500
MAB2271R-01M
IL‑6 R alpha   in Human PBMCs.
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Human IL-6 R alpha Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-6 R alpha in direct ELISAs.
Source
Recombinant Monoclonal Mouse IgG1 Clone # 556704R
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E. coli-derived recombinant human IL-6 R alpha
Arg387-Arg468
Accession # P08887
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
3-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry IL‑6 R alpha   in Human PBMCs. View Larger

IL‑6 R alpha in Human PBMCs. IL‑6 R alpha was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Recombinant Mouse Anti-Human IL‑6 R alpha Monoclonal Antibody (Catalog # MAB2271R) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-6 R alpha

The multi-functional factor interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa component receptor that binds IL-6 with low affinity (IL-6 R alpha ) and a signal-transducing component of 130 kDa (gp130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex (1‑4). Within the extracellular domain, human IL‑6 R alpha shares 52% aa sequence identity with mouse and rat IL‑6 R alpha.

A soluble form of the IL-6 R alpha has been found in the urine of healthy adult humans (5). This soluble receptor apparently arises from proteolytic cleavage of membrane-bound IL-6 R alpha. No naturally-occurring mRNA encoding a truncated form of the IL-6 R alpha has been reported. Soluble forms of human and murine IL-6 R alpha s have been constructed, however, by insertion of termination codons into the regions of the IL-6 R alpha cDNAs encoding the external portions of the receptors and prior to the transmembrane domains. These soluble receptors have been expressed in COS-7 and CHO cells and have been shown to bind to IL-6 in solution and to augment the activity of IL-6 as a result of the binding of the IL‑6/IL‑6 R alpha complex to membrane-bound gp130 (6, 7).

References
  1. Yamasaki et al. (1988) Science 241:825.
  2. Baumann et al. (1990) J. Biol. Chem. 265:19853.
  3. Hibi et al. (1990) Cell 63:1149.
  4. Schooltink et al. (1991) Eur. J. Biochem. 277:659.
  5. Novick et al. (1989) J. Exp. Med. 170:1409.
  6. Yasukawa et al. (1990) J. Biochem. 108:673.
  7. Saito et al. (1991) J. Immunology 147:168.
Long Name
Interleukin 6 Receptor alpha
Entrez Gene IDs
3570 (Human); 16194 (Mouse)
Alternate Names
CD126; gp80; IL-6 R alpha; IL6Q; IL6R alpha; IL6R; IL-6R-1; IL6RA; IL-6Ra; IL6RQ; interleukin 6 receptor

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Cell and Tissue Staining Kits

Isotype Controls

Mouse IgG1 Isotype Control

Ctrl

Reconstitution Buffers

Secondary Antibodies

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