Human Importin  alpha 3/KPNA4 Antibody

Catalog # Availability Size / Price Qty
MAB8204
MAB8204-SP
Detection of Human Importin  alpha 3/KPNA4 by Western Blot.
2 Images
Product Details
FAQs
Supplemental Products
Reviews

Human Importin  alpha 3/KPNA4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Importin alpha 3/KPNA4 in ELISA and Western blot. In direct ELISA, no cross-reactivity to human KPNA3 was detected.
Source
Monoclonal Mouse IgG2B Clone # 843124
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Importin alpha 3/KPNA4
Met1-Asn104
Accession # O00629
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Importin a3/KPNA4 antibody by Western Blot. View Larger

Detection of Human Importin alpha 3/KPNA4 by Western Blot. Western blot shows lysates of NTera-2 human testicular embryonic carcinoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Importin a3/KPNA4 Monoclonal Antibody (Catalog # MAB8204) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Importin a3/KPNA4 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human Importin a3/KPNA4 antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human Importin alpha 3/KPNA4 by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.5 mg/mL. A specific band was detected for Importin alpha 3/KPNA4 at approximately 56 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Importin alpha 3/KPNA4 Monoclonal Antibody (Catalog # MAB8204). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Importin alpha 3/KPNA4

KPNA4 (Karyopherin subunit alpha 4), also called Importin subunit a4 or Importin aQ1 (previously called Qip1 or Importin a3) is an approximately 58-60 kDa member of the Importin alpha family of proteins. It is ubiquitously expressed, and found in both nucleus and cytoplasm. KPNA4 functions as a cargo carrier that transports various complexes from cytoplasm into nucleus.  Human KPNA4 is a 521 amino acid (aa) protein that contains an N-terminal IBB/Importin b domain (aa 2-58), ten Armadillo repeats that bind “cargo” (aa 66-485) and two intervening NLS binding sites. Human KPNA4 aa 1-104 share 99% aa identity with mouse and rat KPNA4, which in turn share 100% identity with each other.

Long Name
Karyopherin alpha 4
Entrez Gene IDs
3840 (Human); 16649 (Mouse); 361959 (Rat)
Alternate Names
Importin alpha 3; Importin alpha Q1; importin subunit alpha-4; importin-alpha-Q1; IPOA3; karyopherin alpha 4 (importin alpha 3); KPNA4; MGC12217; MGC26703; Qip1; QIP12; QIP1Karyopherin subunit alpha-4; SRP3

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Importin  alpha 3/KPNA4 Antibody

There are currently no reviews for this product. Be the first to review Human Importin  alpha 3/KPNA4 Antibody and earn rewards!

Have you used Human Importin  alpha 3/KPNA4 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review