Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot, ELISA Capture

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 639508
View all Integrin alpha 1/CD49a Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived human Integrin alpha 1/CD49a
Phe29-Pro1141
Accession # P56199

Specificity

Detects human Integrin alpha 1/CD49a in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with rhIntegrin alpha 2, 3, 4, 5, 6, 8, 9, 10, 11, L, M, V, X, alpha 6x1/ beta 4, alpha D/ beta 2, alpha 7x2/ beta 1, alpha E/ beta 7, or alpha 2b/ beta 3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Integrin  alpha 1/CD49a Antibody

Detection of Human Integrin a1/CD49a antibody by Western Blot.

Detection of Human Integrin alpha 1/CD49a by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 2 µg/mL of Human Integrin a1/CD49a Monoclonal Antibody (Catalog # MAB5676) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Integrin a1/CD49a at approximately 190 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Integrin alpha 1/CD49a by Western Blot

Detection of Human Integrin alpha 1/CD49a by Western Blot

Levels of HAV RNA at 24 hpi in H1-HeLa cells engineered by CRISPR/Cas9 to be knocked-out for the indicated integrins inoculated with naked HAV (mean ± SD).Immunoblots of whole-cell lysates harvested from the indicated cell lines. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30801249), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Integrin  alpha 1/CD49a Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line

Reviewed Applications

Read 2 reviews rated 4.5 using MAB5676 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Integrin alpha 1/CD49a

Integrin alpha 1 (also VLA-1, CD49a and Laminin and Collagen Receptor) is a 190-210 kDa member of the integrin alpha chain family of molecules.  It is found on smooth muscle cells, osteoblasts, adipocytes and intestinal epithelium.  Integrin alpha 1 forms a noncovalent heterodimer with Integrin beta 1, and serves as a divalent-cation dependent receptor for collagen types I, IV, VI, XIII and XVI.  It also binds the diarrhea-associated NSP4 enterotoxin of rotavirus.  Mature human Integrin alpha 1 is a 1151 amino acid (aa) type I transmembrane glycoprotein that contains a 1113 aa extracellular domain (ECD) and a 15 aa cytoplasmic tail.  The ECD contains one vWFA/I-domain (aa 147-360) that binds collagen, plus multiple divalent cation binding sites.  Potential splice variants exist that show a two and four aa substitution for aa 765-1179.  Over aa 29-1141 (the ECD), human Integrin alpha 1 shares 88% aa identity with mouse Integrin alpha 1. 

Alternate Names

CD49a, ITGA1, VLA-1

Entrez Gene IDs

3672 (Human); 109700 (Mouse); 25118 (Rat)

Gene Symbol

ITGA1

UniProt

P56199

Additional Integrin alpha 1/CD49a Products

Product Documents for Human Integrin  alpha 1/CD49a Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Integrin  alpha 1/CD49a Antibody

For research use only

Citations for Human Integrin  alpha 1/CD49a Antibody

Customer Reviews for Human Integrin  alpha 1/CD49a Antibody (2)

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  • Human Integrin  alpha 1/CD49a Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HeLa cells
    Species: Human
    Verified Customer | Posted 04/06/2022
    HeLa human cervical epithelial carcinoma cell line
    Human Integrin  alpha 1/CD49a Antibody MAB5676
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Huh-7 human hepatoma cell line
    Species: Human
    Verified Customer | Posted 04/30/2018

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