Human Integrin  alpha 1/CD49a Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Integrin alpha 1/CD49a in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with rhIntegrin alpha 2, 3, 4, 5, 6, 8, 9, 10, 11, L, M, V, X, alpha 6x1/ beta 4, alpha D/ beta 2, alpha 7x2/ beta 1, alpha E/ beta 7, or alpha 2b/ beta 3 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 639508
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived human Integrin alpha 1/CD49a
    Phe29-Pro1141
    Accession # P56199
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Integrin alpha 1/CD49a by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 2 µg/mL of Human Integrin alpha 1/CD49a Monoclonal Antibody (Catalog # MAB5676) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Integrin alpha 1/CD49a at approximately 190 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin alpha 1/CD49a

Integrin alpha 1 (also VLA-1, CD49a and Laminin and Collagen Receptor) is a 190-210 kDa member of the integrin alpha chain family of molecules.  It is found on smooth muscle cells, osteoblasts, adipocytes and intestinal epithelium.  Integrin alpha 1 forms a noncovalent heterodimer with Integrin beta 1, and serves as a divalent-cation dependent receptor for collagen types I, IV, VI, XIII and XVI.  It also binds the diarrhea-associated NSP4 enterotoxin of rotavirus.  Mature human Integrin alpha 1 is a 1151 amino acid (aa) type I transmembrane glycoprotein that contains a 1113 aa extracellular domain (ECD) and a 15 aa cytoplasmic tail.  The ECD contains one vWFA/I-domain (aa 147-360) that binds collagen, plus multiple divalent cation binding sites.  Potential splice variants exist that show a two and four aa substitution for aa 765-1179.  Over aa 29-1141 (the ECD), human Integrin alpha 1 shares 88% aa identity with mouse Integrin alpha 1. 

  • Entrez Gene IDs:
    3672 (Human); 109700 (Mouse); 25118 (Rat)
  • Alternate Names:
    CD49 antigen-like family member A; CD49a antigen; CD49a; Integrin alpha 1; integrin, alpha 1; ITGA1; Laminin and collagen receptor; very late activation protein 1; VLA-1; VLA1integrin alpha-1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Pathological lymphangiogenesis is modulated by galectin-8-dependent crosstalk between podoplanin and integrin-associated VEGFR-3
    Authors: WS Chen, Z Cao, S Sugaya, MJ Lopez, VG Sendra, N Laver, H Leffler, UJ Nilsson, J Fu, J Song, L Xia, P Hamrah, N Panjwani
    Nat Commun, 2016;7(0):11302.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

Ctrl MAB002 138  
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Mouse IgG1 Isotype Control

Ctrl MAB002R 1
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse IgG Antibody

WB AF007 4
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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