Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Integrin alpha 11 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Integrin alpha 11
Phe23-Pro1141 (Leu524Arg)
Accession # NP_001004439

Specificity

Detects human Integrin alpha 11 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse Integrin alpha 11 is observed and less than 1% cross-reactivity with recombinant human (rh) Integrin alpha 1 and rhIntegrin alpha 2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Integrin  alpha 11 Antibody

Detection of Human Integrin a11 antibody by Western Blot.

Detection of Human Integrin alpha 11 by Western Blot.

Western blot shows lysates of A549 human lung carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Integrin a11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4235) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Integrin a11 at approximately 155 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human Integrin  alpha 11 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: A549 human lung carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Integrin alpha 11

Integrin alpha 11 (ITGA11) is a 155-160 kDa member of the integrin  alpha chain family of molecules. It preferentially forms a cell surface heterodimer with beta 1 integrin. In particular, it is further classified as a collagen-binding group member, showing a preference for binding to collagen I and II. ITGA11 is expressed by embryonic mesenchymal cells in and around areas that incorporate, or utilize collagen during development. In adult tissue, fibroblasts express ITGA11, and collagen:ITGA11 interaction likely contributes to wound contraction and closure. Mature human ITGA11 is an 1167 amino acid (aa) type I transmembrane glycoprotein. It contains a large 1120 aa extracellular domain (ECD) (aa 23-1142) plus a short 24 aa cytoplasmic region. The ECD shows 7 x 60 aa FG-Gap repeats that generate beta -propellers (aa 24-650) with an intervening I domain that binds collagen (aa 154-345). Over aa 23-1142, human ITGA11 shares 90% aa identity with mouse ITGA11.

Alternate Names

ITGA11

Entrez Gene IDs

22801 (Human); 319480 (Mouse); 315744 (Rat)

Gene Symbol

ITGA11

UniProt

NP_001004439

Additional Integrin alpha 11 Products

Product Documents for Human Integrin  alpha 11 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Integrin  alpha 11 Antibody

For research use only

Citations for Human Integrin  alpha 11 Antibody

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