Human Integrin  alpha 11 Antibody

Catalog # Availability Size / Price Qty
AF4235
AF4235-SP

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Detection of Human Integrin  alpha 11 by Western Blot.
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Product Details
Citations (2)
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Human Integrin  alpha 11 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Integrin alpha 11 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse Integrin alpha 11 is observed and less than 1% cross-reactivity with recombinant human (rh) Integrin alpha 1 and rhIntegrin alpha 2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Integrin alpha 11
Phe23-Pro1141 (Leu524Arg)
Accession # NP_001004439
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Integrin a11 antibody by Western Blot. View Larger

Detection of Human Integrin alpha 11 by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Integrin a11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4235) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Integrin a11 at approximately 155 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 11

Integrin alpha 11 (ITGA11) is a 155-160 kDa member of the integrin  alpha chain family of molecules. It preferentially forms a cell surface heterodimer with beta 1 integrin. In particular, it is further classified as a collagen-binding group member, showing a preference for binding to collagen I and II. ITGA11 is expressed by embryonic mesenchymal cells in and around areas that incorporate, or utilize collagen during development. In adult tissue, fibroblasts express ITGA11, and collagen:ITGA11 interaction likely contributes to wound contraction and closure. Mature human ITGA11 is an 1167 amino acid (aa) type I transmembrane glycoprotein. It contains a large 1120 aa extracellular domain (ECD) (aa 23-1142) plus a short 24 aa cytoplasmic region. The ECD shows 7 x 60 aa FG-Gap repeats that generate beta -propellers (aa 24-650) with an intervening I domain that binds collagen (aa 154-345). Over aa 23-1142, human ITGA11 shares 90% aa identity with mouse ITGA11.

Entrez Gene IDs
22801 (Human); 319480 (Mouse); 315744 (Rat)
Alternate Names
HsT18964; Integrin alpha 11; integrin alpha-11; integrin, alpha 11; ITGA11

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Citations for Human Integrin  alpha 11 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Collagen-rich omentum is a premetastatic niche for integrin alpha 2-mediated peritoneal metastasis
    Authors: Yen-Lin Huang, Ching-Yeu Liang, Danilo Ritz, Ricardo Coelho, Dedy Septiadi, Manuela Estermann et al.
    eLife
  2. Tenascin-X promotes epithelial-to-mesenchymal transition by activating latent TGF-beta.
    Authors: Alcaraz L, Exposito J, Chuvin N, Pommier R, Cluzel C, Martel S, Sentis S, Bartholin L, Lethias C, Valcourt U
    J Cell Biol, 2014-05-12;205(3):409-28.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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