|Detection of Human Integrin alpha 11 by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Integrin alpha 11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4235) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Integrin alpha 11 at approximately 155 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.|
Integrin alpha 11 (ITGA11) is a 155-160 kDa member of the integrin alpha chain family of molecules. It preferentially forms a cell surface heterodimer with beta 1 integrin. In particular, it is further classified as a collagen-binding group member, showing a preference for binding to collagen I and II. ITGA11 is expressed by embryonic mesenchymal cells in and around areas that incorporate, or utilize collagen during development. In adult tissue, fibroblasts express ITGA11, and collagen:ITGA11 interaction likely contributes to wound contraction and closure. Mature human ITGA11 is an 1167 amino acid (aa) type I transmembrane glycoprotein. It contains a large 1120 aa extracellular domain (ECD) (aa 23-1142) plus a short 24 aa cytoplasmic region. The ECD shows 7 x 60 aa FG-Gap repeats that generate beta -propellers (aa 24-650) with an intervening I domain that binds collagen (aa 154-345). Over aa 23-1142, human ITGA11 shares 90% aa identity with mouse ITGA11.
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