Human Integrin  alpha V beta 3 Antibody

Catalog # Availability Size / Price Qty
MAB3050
MAB3050-SP
Product Details
Citations (12)
FAQs
Supplemental Products
Reviews

Human Integrin  alpha V beta 3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Integrin alpha V beta 3.
Source
Monoclonal Mouse IgG1 Clone # 23C6
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human osteoclasts
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
HUVEC human umbilical vein endothelial cells
Immunohistochemistry
Davies, J. et al. (1989) J. Cell Biol. 109:1817.
 
Immunoprecipitation
Davies, J. et al. (1989) J. Cell Biol. 109:1817.
 
Adhesion Blockade
Horton, M.A. et al. (1991) Exp. Cell Res. 195:368. Bates, R.C. et al. (1998) Cell Adhes. Commun. 6:21.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha V beta 3

Integrin alpha V beta 3 together with alpha IIb beta 3, constitutes the only known beta 3 Integrins (1‑3). The non-covalent heterodimer of 170 kDa alpha V/CD51 and 93 kDa beta 3/CD61 subunits shows wide expression, notably by endothelial cells and osteoclasts (2‑4). Each subunit has a transmembrane sequence and a short cytoplasmic tail connected to the cytoskeleton. Active cell surface alpha V beta 3 adheres to matrix proteins including vitronectin, fibronectin, fibrinogen and thrombospondin (2, 3). The ligand binding site of alpha V beta 3 is in the N-terminal head region, formed by interaction of the beta 3 vWFA domain with the alpha V beta-propeller structure (4). The alpha V subunit contributes a thigh and a calf region, while the beta 3 subunit contains a PSI domain and four cysteine-rich I-EGF folds. The alpha V subunit domains termed thigh, calf-1 and calf-2 generate a “knee” region that is bent when the alpha V beta 3 is in its constitutively inactive state. Activation, either by “inside out” signaling or by Mg2+ or Mn2+ binding, extends the Integrin to expose its ligand binding site (1, 4). Two splice variants of beta 3 (b and c) diverge over the last 21 amino acids (aa) and lack cytoplasmic phosphorylation sites (5, 6). Another beta 3 splice variant diverges after the vWFA domain, producing a soluble 60 kDa form in platelets and endothelial cells (7). alpha V beta 3 is essential for the maturation of osteoclasts and their binding and resorption of bone; it also, however, promotes their apoptosis (8, 9). M-CSF R and alpha V beta 3 share signaling pathways during osteoclastogenesis, and deletion of either molecule causes osteopetrosis (8, 9). Also cell entry of several viruses is mediated by alpha V beta 3 (4, 10). The 962 aa human alpha V ECD (11) shares 92‑95% aa sequence identity with mouse, rat and cow  alpha V while the 685 aa human beta 3 ECD (12) shares 95% aa identity with horse and dog, and 89‑92% aa identity with mouse, rat and pig beta 3.

References
  1. Hynes, R. O. (2002) Cell 110:673.
  2. Serini, G. et al. (2006) Exp. Cell Res. 312:651.
  3. Ross, F. P. and S. L. Teitelbaum (2005) Immunol. Rev. 208:88.
  4. Xiong, J. et al. (2001) Science 294:339.
  5. Kumar, C. S. et al. (1987) J. Biol. Chem. 272:16390.
  6. vanKuppevelt, H. et al. (1989) Proc. Natl. Acad. Sci. USA 86:5415.
  7. Djaffar, I. et al. (1994) Biochem. J. 300:67.
  8. McHugh, K. P. et al. (2000) J. Clin. Invest. 105:433.
  9. Faccio, R. et al. (2003) J. Clin. Invest. 111:749.
  10. Chu, J. J. and M. Ng (2004) J. Biol. Chem. 279:54533.
  11. Suzuki, S. et al. (1987) J. Biol. Chem. 262:14060.
  12. Fitzgerald, L. A. et al. (1987) J. Biol. Chem. 262:3936.
Entrez Gene IDs
3685 (Human)
Alternate Names
antigen identified by monoclonal L230; CD51 antigen; CD51; Integrin alpha V beta 3; integrin alpha-V; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); MSK8; Vitronectin receptor subunit alpha; VNRADKFZp686A08142

Product Datasheets

Citations for Human Integrin  alpha V beta 3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. The post-abscission midbody is an intracellular signaling organelle that regulates cell proliferation
    Authors: E Peterman, P Gibieža, J Schafer, VA Skeberdis, A Kaupinis, M Valius, X Heiligenst, I Hurbain, G Raposo, R Prekeris
    Nat Commun, 2019;10(1):3181.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. HLA Class II-Triggered Signaling Cascades Cause Endothelial Cell Proliferation and Migration: Relevance to Antibody-Mediated Transplant Rejection
    Authors: YP Jin, NM Valenzuela, X Zhang, E Rozengurt, EF Reed
    J. Immunol., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  3. Osteopontin-integrin engagement induces HIF-1?-TCF12-mediated endothelial-mesenchymal transition to exacerbate colorectal cancer
    Authors: CS Fan, WS Chen, LL Chen, CC Chen, YT Hsu, KV Chua, HD Wang, TS Huang
    Oncotarget, 2018;9(4):4998-5015.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Acidic preconditioning of endothelial colony-forming cells (ECFC) promote vasculogenesis under proinflammatory and high glucose conditions in vitro and in vivo
    Authors: HA Mena, PR Zubiry, B Dizier, M Schattner, C Boisson-Vi, S Negrotto
    Stem Cell Res Ther, 2018;9(1):120.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. AlphaVBeta3 Integrin expression within uterine endometrium in unexplained infertility: a prospective cohort study
    Authors: A Elnaggar, AH Farag, ME Gaber, MA Hafeez, MS Ali, AM Atef
    BMC Womens Health, 2017;17(1):90.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  6. cRGD peptide installation on cisplatin-loaded nanomedicines enhances efficacy against locally advanced head and neck squamous cell carcinoma bearing cancer stem-like cells
    Authors: K Miyano, H Cabral, Y Miura, Y Matsumoto, Y Mochida, H Kinoh, K Iwata, O Nagano, H Saya, N Nishiyama, K Kataoka, T Yamasoba
    J Control Release, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Osteopontin Promotes Oncostatin M Production in Human Osteoblasts: Implication of Rheumatoid Arthritis Therapy.
    Authors: Su C, Chiang Y, Huang C, Hsu C, Fong Y, Tang C
    J Immunol, 2015;195(7):3355-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Arachidonic acid and docosahexaenoic acid suppress osteoclast formation and activity in human CD14+ monocytes, in vitro.
    Authors: Kasonga A, Deepak V, Kruger M, Coetzee M
    PLoS ONE, 2015;10(4):e0125145.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  9. C6-ceramide nanoliposome suppresses tumor metastasis by eliciting PI3K and PKCzeta tumor-suppressive activities and regulating integrin affinity modulation.
    Authors: Zhang P, Fu C, Hu Y, Dong C, Song Y, Song E
    Sci Rep, 2015;5(0):9275.
  10. Mesenchymal stem cell contact promotes CCN1 splicing and transcription in myeloma cells.
    Authors: Dotterweich J, Ebert R, Kraus S, Tower R, Jakob F, Schutze N
    Cell Commun Signal, 2014;12(0):36.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  11. The nexus between VEGF and NFkappaB orchestrates a hypoxia-independent neovasculogenesis.
    Authors: DeNiro M, Al-Mohanna F, Alsmadi O, Al-Mohanna F
    PLoS ONE, 2013;8(3):e59021.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. The role of RGD-tagged helical rosette nanotubes in the induction of inflammation and apoptosis in human lung adenocarcinoma cells through the P38 MAPK pathway.
    Authors: Suri SS, Rakotondradany F, Myles AJ, Fenniri H, Singh B
    Biomaterials, 2009;30(17):3084-90.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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