Human Integrin  beta 7 Antibody

Catalog # Availability Size / Price Qty
MAB4669
MAB4669-SP

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Detection of Integrin  beta 7 in Human Lymphocytes by Flow Cytometry.
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Product Details
Citations (4)
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Human Integrin  beta 7 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Integrin  beta 7 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Integrin beta 1, beta 2, beta 3, beta 4, beta 5, beta 6, beta 8, recombinant mouse Integrin beta 4, or  beta 7 is observed.
Source
Monoclonal Mouse IgG1 Clone # 473207
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Integrin beta 7 long isoform
Glu20-His723
Accession # P26010
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Integrin  beta 7 antibody in Human Lymphocytes antibody by Flow Cytometry. View Larger

Detection of Integrin beta 7 in Human Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human Integrin beta 7 Monoclonal Antibody (Catalog # MAB4669, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B).

Immunocytochemistry Integrin  beta 7 antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

Integrin beta 7 in Human PBMCs. Integrin beta 7 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human Integrin beta 7 Monoclonal Antibody (Catalog # MAB4669) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 7

Human Integrin beta 7 (also Integrin beta P and M290 IEL Ag) is a 120 kDa member of the Integrin beta chain family of molecules. It is a type I transmembrane glycoprotein that contains a 704 amino acid (aa) extracellular domain (ECD) (aa 20‑723) and a 52 aa cytoplasmic tail. The ECD contains one von Willebrand factor A domain (aa 150‑389) and four cysteine‑rich EGF‑like repeats. One splice variant exists that shows a deletion of aa 501‑648. Integrin beta 7 forms a nondisulfide linked heterodimer with alpha 4 and alpha E integrins. alpha 4 beta 7 binds fibronectin, VCAM-1 and MADCAM1, while alpha E beta 7 binds E-cadherin. The ECD of human beta 7 is 87% aa identical to the ECD of mouse beta 7.

Entrez Gene IDs
3695 (Human); 16421 (Mouse); 25713 (Rat); 102137082 (Cynomolgus Monkey)
Alternate Names
Gut homing receptor beta subunit; integrin beta 7 subunit; Integrin beta 7; integrin beta-7; integrin, beta 7; ITGB7

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Citations for Human Integrin  beta 7 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. The Force-Dependent Mechanism of an Integrin ?4?7-MAdCAM-1 Interaction
    Authors: Su, Y;Luo, Z;Sun, D;Yang, B;Li, Q;
    International journal of molecular sciences
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Selectin Binding Sites Are Involved in Cell Adhesive Properties of Head and Neck Squamous Cell Carcinoma
    Authors: U Valentiner, J Knips, R Pries, T Clauditz, A Münscher, G Sauter, B Wollenberg, U Schumacher
    Cancers (Basel), 2019-10-28;11(11):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Cellular Mechanisms of Etrolizumab Treatment in Inflammatory Bowel Disease
    Authors: C Lichnog, S Klabunde, E Becker, F Fuh, P Tripal, R Atreya, E Klenske, R Erickson, H Chiu, C Reed, S Chung, C Neufert, I Atreya, J McBride, MF Neurath, S Zundler
    Front Pharmacol, 2019-02-01;10(0):39.
    Species: Human
    Sample Types: Whole Cells
  4. A Kaposi's Sarcoma-Associated Herpesvirus Infection Mechanism Is Independent of Integrins alpha 3 beta 1, alpha V beta 3, and alpha V beta 5
    Authors: Allison Alwan TerBush, Florianne Hafkamp, Hee Jun Lee, Laurent Coscoy
    Journal of Virology

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