Detects human IRAK2 in direct ELISAs and Western blots.
In direct ELISAs, no cross-reactivity
with recombinant human (rh) IRAK1, 3, or 4 is observed.
In Western blots, less than 25%
cross-reactivity with rhIRAK1, 3, and 4 is observed.
Monoclonal Mouse IgG3 Clone # 600848
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human IRAK2 Ala2-Asn590 Accession # O43187
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human IRAK2 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, K562 human chronic myelogenous leukemia cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 0.5 µg/mL of Human IRAK2 Monoclonal Antibody (Catalog # MAB6690) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IRAK2 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
IRAK2 is an approximately 75 kDa, 625 amino acid (aa) member of the IL-1 receptor-associated kinase (IRAK) family. The four IRAKs are ser/thr kinases that regulate signaling from Toll-like and IL-1 receptors. IRAKs contain N-terminal death domains and central kinase domains, but activity of the IRAK2 kinase domain is unknown. IRAK1 provides initial, and IRAK2 sustained, NFkB activation and proinflammatory cytokine production in macrophages, acting downstream of IRAK4. In the region used as an immunogen, human IRAK2 shares 69% and 70% amino acid identity with mouse and rat IRAK2, respectively.
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