Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Clone # 600848
View all IRAK2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IRAK2
Ala2-Asn590
Accession # O43187

Specificity

Detects human IRAK2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IRAK1, 3, or 4 is observed. In Western blots, less than 25% cross-reactivity with rhIRAK1, 3, and 4 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Scientific Data Images for Human IRAK2 Antibody

Detection of Human IRAK2 antibody by Western Blot.

Detection of Human IRAK2 by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, K562 human chronic myelogenous leukemia cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 0.5 µg/mL of Human IRAK2 Monoclonal Antibody (Catalog # MAB6690) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IRAK2 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human IRAK2 Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, K562 human chronic myelogenous leukemia cell line, and HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IRAK2

IRAK2 is an approximately 75 kDa, 625 amino acid (aa) member of the IL-1 receptor-associated kinase (IRAK) family. The four IRAKs are ser/thr kinases that regulate signaling from Toll-like and IL-1 receptors. IRAKs contain N-terminal death domains and central kinase domains, but activity of the IRAK2 kinase domain is unknown. IRAK1 provides initial, and IRAK2 sustained, NFkB activation and proinflammatory cytokine production in macrophages, acting downstream of IRAK4. In the region used as an immunogen, human IRAK2 shares 69% and 70% amino acid identity with mouse and rat IRAK2, respectively.

Long Name

Interleukin-1 Receptor-Associated Kinase 2

Alternate Names

interleukin-1 receptor associated kinase-2, interleukin-1 receptor-associated kinase 2, interleukin-1 receptor-associated kinase-like 2, IRAK-2, MGC150550

Entrez Gene IDs

3656 (Human); 108960 (Mouse); 362418 (Rat)

Gene Symbol

IRAK2

UniProt

O43187

Additional IRAK2 Products

Product Documents for Human IRAK2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human IRAK2 Antibody

For research use only

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