|Detection of Human IRF1 by Western Blot. Western blot shows lysates of Nalm‑6 human Pre-B acute lymphocytic leukemia cell line and Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Human IRF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4830) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for IRF1 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 9.|
IRF1 (interferon regulatory factor 1) is a 45 kDa member of the IRF family of proteins. It is induced by TLR9 and INF-gamma stimulation, and positively regulates the expression of IFN-beta, iNOS and 1L-12p35. Human IRF1 is 325 amino acids (aa) in length. It contains an N-terminal DNA-binding basic region (aa 1‑140) and an acidic C-terminus (aa 147‑325). Lys275 and 299 can be SUMOylated, which blocks activity, or ubiquinated at Lys299, which initiates degradation. There is a splice variant that shows an 11 aa substitution for the C-terminal 86 amino acids. Over aa 171‑325, human IRF1 shares 83% aa identity with both canine and porcine IRF1.
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