Human ITIH2 Antibody
Human ITIH2 Antibody Summary
Accession # P19823
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
ITIH2 in SARS-CoV-2 Infected Human Lung. ITIH2 was detected in immersion fixed paraffin-embedded sections of SARS-CoV-2 infected human lung using Rabbit Anti-Human ITIH2 Monoclonal Antibody (Catalog # MAB101992) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
The ITIs are a family of plasma protease inhibitors contributing to extracellular matrix stabilization by covalent linkage to hyaluronan. ITIH2 is a binding protein between hyaluronan and other matrix proteins. It regulates the localization, synthesis and degradation of hyaluronan. ITIH molecules are shown to play a role in carcinogenesis and inflammation. ITIH genes are downregulated in multiple human solid tumors. This downregulation contributes to initiation and/or progression of malignancy.
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