Human JAM-B/VE-JAM Antibody

Catalog # Availability Size / Price Qty
MAB10744-100
MAB10744-SP
Detection of JAM-B/VE-JAM in HEK293 Human Cell Line  Transfected with Human JAM-B/VE-JAM  and eGFP by Flow Cytometry.
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Human JAM-B/VE-JAM Antibody Summary

Species Reactivity
Human
Specificity
Detects human JAM/VE-JAM in direct ELISAs
Source
Monoclonal Mouse IgG2A Clone # 988905
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human JAM‑B/VE‑JAM
Phe29-Asn236
Accession # P57087
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Neutralization
Measured by its ability to neutralize JAM‑B/VE‑JAM-mediated adhesion of the J45.01 human acute lymphoblastic leukemia T lymphocyte cell line. Fong, S. et al. (2002) J. Immunol. 168:1618. The Neutralization Dose (ND50) is typically 35-210 ng/mL in the presence of 0.2 µg/mL Recombinant Human JAM‑B/VE‑JAM Fc Chimera.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of JAM-B/VE-JAM in HEK293 Human Cell Line  Transfected with Human JAM-B/VE-JAM  and eGFP by Flow Cytometry. View Larger

Detection of JAM-B/VE-JAM in HEK293 Human Cell Line Transfected with Human JAM-B/VE-JAM and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human JAM-B/VE-JAM or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human JAM-B/VE-JAM Monoclonal Antibody (Catalog # MAB10744) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.

Neutralization Cell Adhesion Mediated by JAM‑B/VE‑JAM and Neutralization by Human JAM‑B/VE‑JAM Antibody. View Larger

Cell Adhesion Mediated by JAM‑B/VE‑JAM and Neutralization by Human JAM‑B/VE‑JAM Antibody. Recombinant Human JAM‑B/VE‑JAM Fc Chimera (Catalog # 1074-VJ), immobilized onto a microplate supports the adhesion of the J45.01 human acute lymphoblastic leukemia T lymphocyte cell line in a dose-dependent manner (orange line), as measured by Calcein AM. Adhesion elicited by Recombinant Human JAM‑B/VE‑JAM Fc Chimera (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human JAM‑B/VE‑JAM Monoclonal Antibody (Catalog # MAB10744). The ND50 is typically 35-210 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: JAM-B/VE-JAM

The family of juctional adhesion molecules (JAM), comprising at least three members, are type I transmembrane receptors belonging to the immunoglobulin (Ig) superfamily (1, 2). These proteins are localized in the tight junctions between endothelial cells or epithelial cells. Some family members are also found on blood leukocytes and platelets. JAM-B, alternatively named vascular endothelial JAM (VE-JAM), is expressed prominently on high endothelial venules of lymphoid organs where it is localized to the intercellular boundaries of high endothelial cells. It is also expressed on the endothelium of a variety of non-lymphoid organs, especially the heart and placenta (3, 5). Human JAM-B cDNA predicts a 298 amino acid (aa) precursor protein with a putative 28 aa signal peptide, a 209 aa extracellular region containing two Ig domains, a 23 aa transmembrane domain and a 38 aa cytoplasmic domain containing a PDZ-binding motif and a PKC phosphorylation site. Human JAM-B shares approximately 79% aa sequence homology with its mouse homologue. It also shares approximately 35% aa sequence homology with human JAM-A or JAM-C. JAM-B exhibits homotypic interactions, as well as heterotypic interactions with JAM-C, but not JAM-A (4, 5, 7). It is also a ligand for the Integrin alpha 4 beta 1. However, the JAM-B/ alpha 4 beta 1 interaction is facilitated only after prior adhesion of JAM-B to JAM-C (6). Through its heterotypic interactions with JAM-C, JAM-B is an adhesive ligand for T, NK, and dendritic cells, and may play a role in regulating leukocyte transmigration (5).

The nomenclature used for the JAM family proteins is confusing. VE-JAM has been referred in the literature variously as JAM-B or JAM-3. Until further clarification, R&D Systems has adopted the nomenclature where both mouse and human VE-JAM are referred to as JAM-B.

References
  1. Chavakis, T. et al. (2003) Thromb. Haemost. 89:13.
  2. Aurand-Lions, M. et al. (2001) Blood 98:3699.
  3. Palmeri, A. et al. (2000) J. Biol. Chem. 275:19139.
  4. Cunnigham, S. et al. (2000) J. Biol. Chem. 275:34750.
  5. Liang, T. et al. (2002) J. Immunol. 168:1618.
  6. Cunningham, A. et al. (2002) J Biol. Chem. 277:27589.
  7. Arrate, M. et al. (2001) J. Biol. Chem. 276:45826.
Long Name
Junctional Adhesion Molecule B
Entrez Gene IDs
58494 (Human); 67374 (Mouse)
Alternate Names
C21orf43; CD322 antigen; CD322; JAM2; JAMB; JAM-B; JAM-BJAM-IT/VE-JAM; junctional adhesion molecule 2JAM-2; junctional adhesion molecule B; PRO245; Vascular endothelial junction-associated molecule; VE-JAM; VE-JAMVEJAMCD322

Product Datasheets

FAQs

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Isotype Controls

Mouse IgG2A Isotype Control

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Reconstitution Buffers

Secondary Antibodies

Mouse IgG2A Antibody

Mouse IgG2A Antibody

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