Human Kininogen/Kininostatin Antibody

Catalog # Availability Size / Price Qty
AF1396
AF1396-SP
Product Details
Citations (3)
FAQs
Supplemental Products
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Human Kininogen/Kininostatin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Kininogen/Kininostatin in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Kininostatin
Lys438-Ser531
Accession # P01042
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.20 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Kininogen High Molecular Weight (HKa) (Catalog # 1569-PI)
Recombinant Human Kininostatin (Catalog # 1396-KN)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kininogen/Kininostatin

High molecular weight kininogen (HK) is a secreted plasma glycoprotein consisting of six domains (D1 through D6). It binds specifically to endothelial cells and serves as a substrate of plasma kallikrein which releases bradykinin from within D4 of HK. Bradykinin is an active peptide that regulates multiple vascular processes. The resulting cleaved HK, called HKa, is a disulfide-linked dimer containing a heavy chain (D1 to D3) and a light chain (D5 and D6). HKa differs from HK in conformation and possesses new functional properties. It is a potent angiogenesis inhibitor that strongly inhibits endothelial cell proliferation and migration. HKa also induces apoptosis of endothelial cells. D5 of HKa has been shown to be the active region primarily responsible for the HKa anti-angiogenic activity and has been named kininostatin. While both HK and HKa are plasma proteins that are present in circulation, it is not known if kininostatin (D5) can be released from HKa and exist in plasma (1-4).

References
  1. Guo, Y. et al. (2002) International Immunopharmacology 2:1931.
  2. Zhang, J. et al. (2000) FASEB 14:2589.
  3. Colman, R.W. et al. (2000) Blood 95(2): 543.
  4. Guo, Y. et al. (2001) Arterioscler. Thromb. Vasc. Biol. 21:1427.
Entrez Gene IDs
3827 (Human); 16644 (Mouse); 24903 (Rat)
Alternate Names
Alpha-2-thiol proteinase inhibitor; BDK; bradykinin; Fitzgerald factor; High molecular weight kininogen; kininogen 1; kininogen; Kininogen/Kininostatin; kininogen-1; KNGHMWK; Williams-Fitzgerald-Flaujeac factor

Product Datasheets

Citations for Human Kininogen/Kininostatin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. High molecular weight kininogen binds to laminin--characterization and kinetic analysis.
    Authors: Schousboe I, Nystrom B
    FEBS J., 2009;276(18):5228-38.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Western Blot
  2. Aberrant processing of plasma vitronectin and high-molecular-weight kininogen precedes the onset of preeclampsia.
    Authors: Blumenstein M, Prakash R, Cooper GJ
    Reprod Sci, 2009;16(12):1144-52.
    Species: Human
    Sample Types: Plasma
    Applications: Western Blot
  3. Inactivation of plasminogen activator inhibitor type 1 by activated factor XII plays a role in the enhancement of fibrinolysis by contact factors in-vitro.
    Authors: Tanaka A, Suzuki Y, Sugihara K, Kanayama N, Urano T
    Life Sci., 2009;85(5):220-5.
    Species: Human
    Sample Types: Plasma
    Applications: Western Blot

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Secondary Antibodies

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