Human KIR2DL1/KIR2DS5 Antibody

Catalog # Availability Size / Price Qty
MAB1844-SP
MAB1844-500
MAB1844-100
Detection of KIR2DL1/KIR2DS5 in Human PBMCs by Flow Cytometry.
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Product Details
Citations (16)
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Human KIR2DL1/KIR2DS5 Antibody Summary

Species Reactivity
Human
Specificity
Detects human KIR2DL1/KIR2DS5 on transfected cells. It does not stain cells transfected with KIR2DL2, 2DL3, 2DL4, 2DL5, 2DS1, 2DS2, 2DS4, 3DL1, 3DL2, or 3DS1.
Source
Monoclonal Mouse IgG1 Clone # 143211
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF3 mouse pro-B cell line transfected with human KIR2DL1/KIR2DS5
Accession # P43626
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-reported
Horowitz, A. et al. (2015) J. Immunol. 195: 4524. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of KIR2DL1/KIR2DS5 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of KIR2DL1/KIR2DS5 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with (A) Mouse Anti-Human KIR2DL1/KIR2DS5 Monoclonal Antibody (Catalog # MAB1844) or (B) Mouse IgG1 Isotype Control Antibody (Catalog # MAB002) followed by Anti-Mouse IgG APC-conjugated Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: KIR2DL1/KIR2DS5

KIR2DL1 is one of several immunoglobulin-like receptors expressed on NK cells that bind MHC class I molecules and transmit inhibitory signals. KIR2DL1 is specific for HLA-C alleles with Asn77 and Lys80.

Alternate Names
CD158a; KIR2DL1/KIR2DS5

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Citations for Human KIR2DL1/KIR2DS5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. Deciphering the biology of KIR2DL3+ T lymphocytes that are associated to relapse in haploidentical HSCT
    Authors: G David, C Willem, N Legrand, Z Djaoud, P Mérieau, A Walencik, T Guillaume, K Gagne, P Chevallier, C Retière
    Scientific Reports, 2021-08-04;11(1):15782.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Centromeric KIR AA Individuals Harbor Particular KIR Alleles Conferring Beneficial NK Cell Features with Implications in Haplo-Identical Hematopoietic Stem Cell Transplantation
    Authors: L Dubreuil, B Maniangou, P Chevallier, A Quéméner, N Legrand, MC Béné, C Willem, G David, M Alizadeh, DR Makanga, A Cesbron, K Gendzekhad, K Gagne, C Retière
    Cancers, 2020-12-01;12(12):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. KIR+ CD8+ T Lymphocytes in Cancer Immunosurveillance and Patient Survival: Gene Expression Profiling
    Authors: L Gimeno, EM Serrano-Ló, JA Campillo, MA Cánovas-Za, OS Acuña, F García-Cóz, MV Martínez-S, MD Martínez-H, MF Soto-Ramír, P López-Cubi, J Martínez-E, J Martínez-G, S Corbalan-G, MR Álvarez-Ló, A Minguela
    Cancers (Basel), 2020-10-15;12(10):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Missing self triggers NK cell-mediated chronic vascular rejection of solid organ transplants
    Authors: A Koenig, CC Chen, A Marçais, T Barba, V Mathias, A Sicard, M Rabeyrin, M Racapé, JP Duong-Van-, P Bruneval, A Loupy, S Dussurgey, S Ducreux, V Meas-Yedid, JC Olivo-Mari, H Paidassi, R Guillemain, JL Taupin, J Callemeyn, E Morelon, A Nicoletti, B Charreau, V Dubois, M Naesens, T Walzer, T Defrance, O Thaunat
    Nat Commun, 2019-11-25;10(1):5350.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Contribution of NK cell education to both direct and anti-HIV-1 antibody-dependent NK cell functions
    Authors: AB Kristensen, SJ Kent, MS Parsons
    J. Virol., 2018-05-14;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Clinical impact of NK-cell reconstitution after reduced intensity conditioned unrelated cord blood transplantation in patients with acute myeloid leukemia: analysis of a prospective phase II multicenter trial on behalf of the Soci�t� Fran�aise de Greffe de Moelle Osseuse et Th�rapie Cellulaire and Eurocord
    Authors: S Nguyen, A Achour, L Souchet, S Vigouroux, P Chevallier, S Furst, A Sirvent, JO Bay, G Socié, P Ceballos, A Huynh, J Cornillon, S Francois, F Legrand, I Yakoub-Agh, G Michel, N Maillard, G Margueritt, S Maury, M Uzunov, CE Bulabois, M Michallet, L Clement, C Dauriac, K Bilger, J Lejeune, V Béziat, V Rocha, B Rio, S Chevret, V Vieillard
    Bone Marrow Transplant., 2017-06-26;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Severe symptomatic primary HCMV infection despite effective innate and adaptive immune responses
    Authors: Raphaëlle Riou
    J. Virol, 2017-02-14;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Activating Killer Immunoglobulin Receptors and HLA-C: a successful combination providing HIV-1 control
    Authors: MS Malnati, E Ugolotti, MC Monti, D Battista, I Vanni, D Bordo, F Sironi, P Larghero, ED Marco, P Biswas, G Poli, E Vicenzi, A Riva, M Tarkowski, G Tambussi, S Nozza, G Tripodi, F Marras, A Maria, A Pistorio, R Biassoni
    Sci Rep, 2017-02-13;7(0):42470.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Activating KIR2DS4 Is Expressed by Uterine NK Cells and Contributes to Successful Pregnancy
    Authors: Ashley Moffett
    J. Immunol., 2016-11-04;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Regulation of Adaptive NK Cells and CD8 T Cells by HLA-C Correlates with Allogeneic Hematopoietic Cell Transplantation and with Cytomegalovirus Reactivation.
    Authors: Horowitz A, Guethlein L, Nemat-Gorgani N, Norman P, Cooley S, Miller J, Parham P
    J Immunol, 2015-09-28;195(9):4524-36.
    Species: Human
    Sample Types: Whole Cells
    Applications: CyTof
  11. Coordinated expression of DNAM-1 and LFA-1 in educated NK cells.
    Authors: Enqvist M, Ask E, Forslund E, Carlsten M, Abrahamsen G, Beziat V, Andersson S, Schaffer M, Spurkland A, Bryceson Y, Onfelt B, Malmberg K
    J Immunol, 2015-03-30;194(9):4518-27.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Ascorbic acid promotes proliferation of natural killer cell populations in culture systems applicable for natural killer cell therapy.
    Authors: Huijskens M, Walczak M, Sarkar S, Atrafi F, Senden-Gijsbers B, Tilanus M, Bos G, Wieten L, Germeraad W
    Cytotherapy, 2015-03-05;17(5):613-20.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Modulation of the natural killer cell KIR repertoire by cytomegalovirus infection.
    Authors: Charoudeh H, Terszowski G, Czaja K, Gonzalez A, Schmitter K, Stern M
    Eur J Immunol, 2012-12-12;43(2):480-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Membrane-bound IL-21 promotes sustained ex vivo proliferation of human natural killer cells.
    Authors: Denman CJ, Senyukov VV, Somanchi SS
    PLoS ONE, 2012-01-18;7(1):e30264.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. NK cell terminal differentiation: correlated stepwise decrease of NKG2A and acquisition of KIRs.
    Authors: Beziat V, Descours B, Parizot C, Debre P, Vieillard V
    PLoS ONE, 2010-08-06;5(8):e11966.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Significant functional heterogeneity among KIR2DL1 alleles and a pivotal role of arginine 245.
    Authors: Bari R, Bell T, Leung WH, Vong QP, Chan WK, Das Gupta N, Holladay M, Rooney B, Leung W
    Blood, 2009-12-10;114(25):5182-90.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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