Human KIR2DS1/CD158h Antibody

Catalog #: MAB8887 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB8887
MAB8887-SP

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Detection of KIR2DS1/CD158h in Human PBMCs by Flow Cytometry.

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Human KIR2DS1/CD158h Antibody Summary

Species Reactivity

Human

Specificity

Detects human KIR2DS1/CD158h in flow cytometry. Clone 1127B recognizes KIR2DS1 and some alleles of KIR2DL1, both members of the killer cell immunoglobulin-like receptor (KIR) family. Because Clone 1127B displays partial cross-reactivity with KIR2DL1, co-staining with Mouse Anti-Human KIR2DL1 Clone 143211 (Catalog # FAB1844F) is recommended.

Source

Recombinant Monoclonal Rabbit IgG Clone # 1127B

Purification

Protein A or G purified from cell culture supernatant

Immunogen

HEK293 human embryonic kidney cell line transfected with human KIR2DS1/CD158h
Met1-His245
Accession # Q14954

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of KIR2DS1/CD158h in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) from different donors (A and B) were stained with Rabbit Anti-Human KIR2DS1/CD158h Monoclonal Antibody (Catalog # MAB8887) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111) and Mouse Anti-Human KIR2DL1/KIR2DS5 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1844F). Cells were gated on CD56 with Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P, not shown).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: KIR2DS1/CD158h

The KIRs comprise a family of 14 polymorphic and homologous activating and inhibitory receptors expressed primarily on CD56^dim NK cells. KIR2DS1 is an activating receptor with high homology to the inhibitory receptor KIR2DL1. Both KIR2DL1 and KIR2DS1 bind to HLA-C alleles containing the C2 epitope. KIR2DS1 and KIR2DL1 expression regulates NK cell licensing and activation in a number of immune contexts.

Long Name

Killer Cell Immunoglobulin-like Receptor, Two Domains, Short Cytoplasmic Tail, 1

Entrez Gene IDs

3806 (Human)

Alternate Names

killer cell immunoglobulin-like receptor 2DS1; CD158a; CD158h; EB6ActI; killer cell immunoglobulin-like receptor, two domains, short cytoplasmic tail, 1; KIR2DS1; p50.1