Detects human KIR2DS1/CD158h in flow cytometry. Clone 1127B recognizes KIR2DS1 and some alleles of KIR2DL1, both members of the killer cell immunoglobulin-like receptor (KIR) family. Because Clone 1127B displays partial cross-reactivity with KIR2DL1, co-staining with Mouse Anti-Human KIR2DL1 Clone 143211 (Catalog # FAB1844F) is recommended.
Recombinant Monoclonal Rabbit IgG Clone # 1127B
Protein A or G purified from cell culture supernatant
HEK293 human embryonic kidney cell line transfected with human KIR2DS1/CD158h Met1-His245 Accession # Q14954
Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
0.25 µg/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of KIR2DS1/CD158h in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) from different donors (A and B) were stained with Rabbit Anti-Human KIR2DS1/CD158h Monoclonal Antibody (Catalog # MAB8887) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111) and Mouse Anti-Human KIR2DL1/KIR2DS5 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB1844F). Cells were gated on CD56 with Mouse Anti-Human NCAM‑1/CD56 PE‑conjugated Monoclonal Antibody (Catalog # FAB2408P, not shown).
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 °C, as supplied.
1 month, 2 to 8 °C under sterile conditions after opening.
6 months, -20 to -70 °C under sterile conditions after opening.
The KIRs comprise a family of 14 polymorphic and homologous
activating and inhibitory receptors expressed primarily on CD56dim
NK cells. KIR2DS1 is an activating receptor with high homology to the
inhibitory receptor KIR2DL1. Both KIR2DL1 and KIR2DS1 bind to HLA-C alleles
containing the C2 epitope. KIR2DS1 and KIR2DL1 expression regulates NK cell
licensing and activation in a number of immune contexts.
Killer Cell Immunoglobulin-like Receptor, Two Domains, Short Cytoplasmic Tail, 1
Entrez Gene IDs:
killer cell immunoglobulin-like receptor 2DS1; CD158a; CD158h; EB6ActI; killer cell immunoglobulin-like receptor, two domains, short cytoplasmic tail, 1; KIR2DS1; p50.1
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