Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

CyTof, CyTOF-ready

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1127B
View all KIR2DS1/CD158h Primary Antibodies »

Product Specifications

Immunogen

HEK293 human embryonic kidney cell line transfected with human KIR2DS1/CD158h
Met1-His245
Accession # Q14954

Specificity

Detects human KIR2DS1/CD158h in flow cytometry. Clone 1127B recognizes KIR2DS1 and some alleles of KIR2DL1, both members of the killer cell immunoglobulin-like receptor (KIR) family. Because Clone 1127B displays partial cross-reactivity with KIR2DL1, co-staining with Mouse Anti-Human KIR2DL1 Clone 143211 (Catalog # FAB1844F) is recommended.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human KIR2DS1/CD158h Antibody

Detection of KIR2DS1/CD158h antibody in Human PBMCs antibody by Flow Cytometry.

Detection of KIR2DS1/CD158h in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) from different donors (A and B) were stained with Rabbit Anti-Human KIR2DS1/CD158h Monoclonal Antibody (Catalog # MAB8887) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111) and Mouse Anti-Human KIR2DL1/KIR2DS5 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1844F). Cells were gated on CD56 with Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P, not shown).

Applications for Human KIR2DS1/CD158h Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs)

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: KIR2DS1/CD158h

The KIRs comprise a family of 14 polymorphic and homologous activating and inhibitory receptors expressed primarily on CD56dim NK cells. KIR2DS1 is an activating receptor with high homology to the inhibitory receptor KIR2DL1. Both KIR2DL1 and KIR2DS1 bind to HLA-C alleles containing the C2 epitope. KIR2DS1 and KIR2DL1 expression regulates NK cell licensing and activation in a number of immune contexts.

Long Name

Killer Cell Immunoglobulin-like Receptor, Two Domains, Short Cytoplasmic Tail, 1

Alternate Names

CD158h, EB6ActI

Entrez Gene IDs

3806 (Human)

Gene Symbol

KIR2DS1

UniProt

Q14954

Additional KIR2DS1/CD158h Products

Product Documents for Human KIR2DS1/CD158h Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human KIR2DS1/CD158h Antibody

For research use only

Citations for Human KIR2DS1/CD158h Antibody

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