Detection of KIR3DL3/CD158z in BaF3 Mouse Cell Line Transfected with Human KIRDL3/CD158z by Flow Cytometry. BaF3 mouse pro-B cell line transfected with human KIRDL3/CD158z was stained with Rabbit Anti-Human KIR3DL3/CD158z Monoclonal Antibody (Catalog # MAB8919, filled histogram) or Rabbit Anti-Human KIR2DS5/CD158g Monoclonal Antibody (Catalog # MAB8920, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111).
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 °C, as supplied.
1 month, 2 to 8 °C under sterile conditions after opening.
6 months, -20 to -70 °C under sterile conditions after opening.
KIR3DL3 is a member of the killer-immunoglobulin-like receptors (KIR), a functionally diverse family of transmembrane receptors expressed primarily on NK cells. KIR3DL3 is a “framework” KIR gene, and is present in all individuals, in contrast to non-framework KIRs, whose gene content varies by KIR haplotype. KIR3DL3 is expressed at low or undetectable levels in peripheral blood NK cells, but expression can be induced in NK cell lines by demethylation agents, indicating that promoter DNA methylation inhibits KIR3DL3 transcription. Despite the lack of evidence for surface protein expression, KIR3DL3 mRNA expression has been detected in both decidual and peripheral blood NK cells in normal, disease-free conditions.
Killer Cell Immunoglobulin-like Receptor, Three Domain Long Cytoplasmic Tail, 3
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