Human KIR3DL3/CD158z Antibody

Recombinant Monoclonal Antibody
    
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  • Species Reactivity
    Human
  • Specificity
    Detects human KIR3DL3/CD158z in direct ELISAs.
  • Source
    Recombinant Monoclonal Rabbit IgG Clone # 1136B
  • Purification
    Protein A or G purified from cell culture supernatant
  • Immunogen
    Human embryonic kidney cell line HEK293-derived recombinant human KIR3DL3/CD158z
    Met1-Leu322
    Accession # Q8N743
  • Formulation
    Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    1:1000 dilution
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of KIR3DL3/CD158z in BaF3 Mouse Cell Line Transfected with Human KIRDL3/CD158z by Flow Cytometry. BaF3 mouse pro-B cell line transfected with human KIRDL3/CD158z was stained with Rabbit Anti-Human KIR3DL3/CD158z Monoclonal Antibody (Catalog # MAB8919, filled histogram) or Rabbit Anti-Human KIR2DS5/CD158g Monoclonal Antibody (Catalog # MAB8920, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111).
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 °C, as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after opening.
    • 6 months, -20 to -70 °C under sterile conditions after opening.
Background: KIR3DL3/CD158z
KIR3DL3 is a member of the killer-immunoglobulin-like receptors (KIR), a functionally diverse family of transmembrane receptors expressed primarily on NK cells. KIR3DL3 is a “framework” KIR gene, and is present in all individuals, in contrast to non-framework KIRs, whose gene content varies by KIR haplotype. KIR3DL3 is expressed at low or undetectable levels in peripheral blood NK cells, but expression can be induced in NK cell lines by demethylation agents, indicating that promoter DNA methylation inhibits KIR3DL3 transcription. Despite the lack of evidence for surface protein expression, KIR3DL3 mRNA expression has been detected in both decidual and peripheral blood NK cells in normal, disease-free conditions.
  • Long Name:
    Killer Cell Immunoglobulin-like Receptor, Three Domain Long Cytoplasmic Tail, 3
  • Entrez Gene IDs:
    115653 (Human)
  • Alternate Names:
    CD158z; KIR3DL3; KIR3DL7; KIR44; KIRC1
Related Research Areas

FAQs

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Isotype Controls
Description Application Cat# Citations Images  

Normal Rabbit IgG Control

Ctrl AB-105-C 46  
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Normal Rabbit IgG Control

Ctrl , CyTOF-ready MAB1050  
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Secondary Antibodies
Description Application Cat# Citations Images  

Rabbit IgG HRP-conjugated Antibody

WB HAF008 35  
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Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Antibody

Flow , IHC , ICC NL004 10
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Rabbit IgG PE-conjugated Antibody

Flow F0110 2  
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Donkey Anti-Rabbit IgG NorthernLights™ NL637-conjugated Antibody

Flow , IHC , ICC NL005 2
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Rabbit IgG APC-conjugated Antibody

Flow F0111 1  
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Donkey Anti-Rabbit IgG NorthernLights™ NL493-conjugated Antibody

Flow , IHC , ICC NL006 4
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Rabbit IgG Fluorescein-conjugated Antibody

Flow F0112 5  
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Goat Anti-Rabbit IgG Biotinylated Antibody

WB BAF008 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002 1  
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Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC003  
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Rabbit IgG Antibody

WB AF008 1
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Donkey Anti-Rabbit IgG (H+L) Antibody

D-301-C-ABS2
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