Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1136B
View all KIR3DL3/CD158z Primary Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell line HEK293-derived recombinant human KIR3DL3/CD158z
Met1-Leu322
Accession # Q8N743

Specificity

Detects human KIR3DL3/CD158z in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human KIR3DL3/CD158z Antibody

Detection of KIR3DL3/CD158z antibody in BaF3 Mouse Cell Line Transfected with Human KIRDL3/CD158z antibody by Flow Cytometry.

Detection of KIR3DL3/CD158z in BaF3 Mouse Cell Line Transfected with Human KIRDL3/CD158z by Flow Cytometry.

BaF3 mouse pro-B cell line transfected with human KIRDL3/CD158z was stained with Rabbit Anti-Human KIR3DL3/CD158z Monoclonal Antibody (Catalog # MAB8919, filled histogram) or Rabbit Anti-Human KIR2DS5/CD158g Monoclonal Antibody (Catalog # MAB8920, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111).
Detection of KIR3DL3/CD158z by Western Blot

Detection of KIR3DL3/CD158z by Western Blot

Suppression of PPAR gamma expression in differentiating preadipocytes. Expression level of PPAR gamma in preadipocytes on d0, d6 post-differentiation (d6), and d6 post-differentiation in the presence of (A) berberine (C1), (B) luteolin (C2), (C) resveratrol (C3), (D) fisetin (C4), (E) quercetin (C5), (F) fucoidan (C6), (G) EGCG (C7), (H) hesperidin (C8), (I) curcumin (C9), or (J) F1 composition. beta -actin served as a loading control. The protein expression level was measured using multiplexed capillary Western immunoassays. Molecular markers in kilodalton (kDa) are displayed on the left of the capillary Western immunoassay data. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36297009), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of KIR3DL3/CD158z by Western Blot

Detection of KIR3DL3/CD158z by Western Blot

Suppression of PPAR gamma expression in differentiating preadipocytes. Expression level of PPAR gamma in preadipocytes on d0, d6 post-differentiation (d6), and d6 post-differentiation in the presence of (A) berberine (C1), (B) luteolin (C2), (C) resveratrol (C3), (D) fisetin (C4), (E) quercetin (C5), (F) fucoidan (C6), (G) EGCG (C7), (H) hesperidin (C8), (I) curcumin (C9), or (J) F1 composition. beta -actin served as a loading control. The protein expression level was measured using multiplexed capillary Western immunoassays. Molecular markers in kilodalton (kDa) are displayed on the left of the capillary Western immunoassay data. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36297009), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human KIR3DL3/CD158z Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: BaF3 mouse pro-B cell line transfected with human KIRDL3/CD158z

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: KIR3DL3/CD158z

KIR3DL3 is a member of the killer-immunoglobulin-like receptors (KIR), a functionally diverse family of transmembrane receptors expressed primarily on NK cells. KIR3DL3 is a “framework” KIR gene, and is present in all individuals, in contrast to non-framework KIRs, whose gene content varies by KIR haplotype. KIR3DL3 is expressed at low or undetectable levels in peripheral blood NK cells, but expression can be induced in NK cell lines by demethylation agents, indicating that promoter DNA methylation inhibits KIR3DL3 transcription. Despite the lack of evidence for surface protein expression, KIR3DL3 mRNA expression has been detected in both decidual and peripheral blood NK cells in normal, disease-free conditions.

Long Name

Killer Cell Immunoglobulin-like Receptor, Three Domain Long Cytoplasmic Tail, 3

Alternate Names

CD158z, KIR3DL7, KIR44, KIRC1

Entrez Gene IDs

115653 (Human)

Gene Symbol

KIR3DL3

UniProt

Q8N743

Additional KIR3DL3/CD158z Products

Product Documents for Human KIR3DL3/CD158z Antibody

Certificate of Analysis

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Product Specific Notices for Human KIR3DL3/CD158z Antibody

For research use only

Citations for Human KIR3DL3/CD158z Antibody

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