Human Ku70/XRCC6 Antibody

Catalog # Availability Size / Price Qty
AF5597
AF5597-SP
Detection of Human Ku70/XRCC6 by Western Blot.
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Human Ku70/XRCC6 Antibody Summary

Species Reactivity
Human
Specificity
Detects endogenous human Ku70 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Ku70
Asn405-Lys556
Accession # P12956
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Ku70/XRCC6 antibody by Western Blot. View Larger

Detection of Human Ku70/XRCC6 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, MCF-7 human breast cancer cell line, and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Human Ku70/XRCC6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5597) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Ku70/XRCC6 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ku70/XRCC6

Ku70 (Lupus Ku autoantigen protein 70; also ATP-dependent DNA helicase 2 subunit 1) is a ubiquitous 70‑75 kDa nuclear protein that serves as a component of the DNA-dependent protein kinase complex DNA-PK. In particular, it heterodimerizes with Ku80 to form a complex that binds DNA ends and select sequences, and which recruits DNA-PKcs for the purpose of phosphorylating multiple chromatin-bound proteins, including p53, Oct-1 and Sp-1. The Ku:DNA-PKcs complex participates in DNA repair, DNA replication, and VDJ recombination. Human Ku70 is 609 amino acids (aa) in length. It contains a Ku core domain (aa 266‑529), a DNA binding motif (aa 268‑274), an NLS (aa 539‑556) and a C-terminal SAP domain (aa 573‑607). There is one potential splice form that shows a deletion of aa 66‑106. Over aa 405‑556, Ku70 shares 83% aa identity with mouse Ku70.

Long Name
Lupus Ku Autoantigen Protein 70
Entrez Gene IDs
2547 (Human)
Alternate Names
5'-deoxyribose-5-phosphate lyase Ku70; 5'-dRP lyase Ku70; 70 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 1; ATP-dependent DNA helicase II 70 kDa subunit; CTC box binding factor 75 kDa subunit; CTC box-binding factor 75 kDa subunit; CTC75; CTCBF; D22S731; EC 3.6.4.-; EC 4.2.99.-; G22P1; G22P1Ku70; Ku autoantigen p70 subunit; Ku autoantigen, 70kDa; Ku70; ML8; ML8,70 kDa subunit; thyroid-lupus autoantigen p70; Thyroid-lupus autoantigen; TLAA; TLAAD22S671; X-ray repair complementing defective repair in Chinese hamster cells 6DNA repair protein XRCC6; X-ray repair cross-complementing protein 6; XRCC6

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody

WB,ELISA

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