|Detection of Human Ku70/XRCC6 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, MCF‑7 human breast cancer cell line, and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Human Ku70/XRCC6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5597) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Ku70/XRCC6 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
Ku70 (Lupus Ku autoantigen protein 70; also ATP-dependent DNA helicase 2 subunit 1) is a ubiquitous 70‑75 kDa nuclear protein that serves as a component of the DNA-dependent protein kinase complex DNA-PK. In particular, it heterodimerizes with Ku80 to form a complex that binds DNA ends and select sequences, and which recruits DNA-PKcs for the purpose of phosphorylating multiple chromatin-bound proteins, including p53, Oct-1 and Sp-1. The Ku:DNA-PKcs complex participates in DNA repair, DNA replication, and VDJ recombination. Human Ku70 is 609 amino acids (aa) in length. It contains a Ku core domain (aa 266‑529), a DNA binding motif (aa 268‑274), an NLS (aa 539‑556) and a C-terminal SAP domain (aa 573‑607). There is one potential splice form that shows a deletion of aa 66‑106. Over aa 405‑556, Ku70 shares 83% aa identity with mouse Ku70.
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