Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 901258
View all L1TD1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human L1TD1
Met1-Leu142
Accession # Q5T7N2

Specificity

Detects human L1TD1 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human L1TD1 Antibody

Detection of Human L1TD1 antibody by Western Blot.

Detection of Human L1TD1 by Western Blot.

Western blot shows lysates of BG01V human embryonic stem cells and NTera-2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human L1TD1 Monoclonal Antibody (Catalog # MAB8317) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for L1TD1 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

L1TD1 antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC).

L1TD1 in BG01V Human Embryonic Stem Cells.

L1TD1 was detected in immersion fixed BG01V human embryonic stem cells, undifferentiated (upper panel) or differentiated for 4 days (lower panel), using Mouse Anti-Human L1TD1 Monoclonal Antibody (Catalog # MAB8317) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Applications for Human L1TD1 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells

Western Blot

1 µg/mL
Sample: BG01V human embryonic stem cells and NTera-2 human testicular embryonic carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: L1TD1

L1TD1 (LINE-1 type transposase domain-containing protein 1; also known as ES cell-associated protein 11 and FLJ10884) is an RNA binding protein with a reported molecular weight of approximately 100 kDa.  It is 865 amino acids (aa) in length and shares 43% aa identity with mouse L1TD1.  L1TD1 is a marker for undifferentiated pluripotent stem cells.  Knock down of its expression in these cells has been shown to decrease the expression of critical pluripotency factors such as Nanog and Oct-3/4.  L1TD1 function in stem cells is likely regulated by its interaction with factors such as LIN-28 and is suggested to regulate RNA processing.

Long Name

LINE-1 Type Transposase Domain Containing 1

Alternate Names

ECAT11

Entrez Gene IDs

54596 (Human); 381591 (Mouse); 685355 (Rat)

Gene Symbol

L1TD1

UniProt

Q5T7N2

Additional L1TD1 Products

Product Documents for Human L1TD1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human L1TD1 Antibody

For research use only

Citations for Human L1TD1 Antibody

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Protocols

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