Human L1TD1 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human L1TD1 in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 901258
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human L1TD1
    Met1-Leu142
    Accession # Q5T7N2
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human L1TD1 by Western Blot. Western blot shows lysates of BG01V human embryonic stem cells and NTera-2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human L1TD1 Monoclonal Antibody (Catalog # MAB8317) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for L1TD1 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
L1TD1 in BG01V Human Embryonic Stem Cells. L1TD1 was detected in immersion fixed BG01V human embryonic stem cells, undifferentiated (upper panel) or differentiated for 4 days (lower panel), using Mouse Anti-Human L1TD1 Monoclonal Antibody (Catalog # MAB8317) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: L1TD1
L1TD1 (LINE-1 type transposase domain-containing protein 1; also known as ES cell-associated protein 11 and FLJ10884) is an RNA binding protein with a reported molecular weight of approximately 100 kDa.  It is 865 amino acids (aa) in length and shares 43% aa identity with mouse L1TD1.  L1TD1 is a marker for undifferentiated pluripotent stem cells.  Knock down of its expression in these cells has been shown to decrease the expression of critical pluripotency factors such as Nanog and Oct-3/4.  L1TD1 function in stem cells is likely regulated by its interaction with factors such as LIN-28 and is suggested to regulate RNA processing.
  • Long Name:
    LINE-1 Type Transposase Domain Containing 1
  • Entrez Gene IDs:
    54596 (Human); 381591 (Mouse); 685355 (Rat)
  • Alternate Names:
    ECAT11; ES cell-associated protein 11; FLJ10884; L1TD1; LINE-1 type transposase domain containing 1; LINE-1 type transposase domain-containing protein 1; MGC133253; RP5-1155K23.3
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