Human Lactase-like Protein/LCTL Antibody

Catalog #: AF5984 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5984
AF5984-SP

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Detection of Human Lactase-like Protein/LCTL by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Lactase-like Protein/LCTL Antibody Summary

Species Reactivity

Human

Specificity

Detects human Lactase-like Protein/LCTL in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross‑reactivity with recombinant mouse Lactase-like Protein/LCTL is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Lactase-like Protein/LCTL
Arg23-Glu541
Accession # Q6UWM7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Lactase-like Protein/LCTL, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Lactase-like Protein/LCTL by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line and A431 human epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Lactase-like Protein/LCTL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5984) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Lactase-like Protein/LCTL at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lactase-like Protein/LCTL

LCTL (also Lactase-like Protein and Klotho/lactase-phlorizin hydrolase-related protein/KLPH) is a 62 kDa (predicted) member of the glycosyl hydrolase 1 family of proteins. It is expressed in skin and kidney, and may participate in endocrine FGF19 subfamily signaling pathways. Mature human LCTL is a 546 amino acid (aa) type I transmembrane protein that appears to be present in the ER. It contains an apparently nonfunctional glycosidase domain (aa 33‑503), plus a very short five aa cytoplasmic segment that may function as an ER-retention signal. There is one potential alternative start site at Met174. Over aa 23‑541, human LCTL shares 86% aa identity with mouse LCTL.

Entrez Gene IDs

197021 (Human); 235435 (Mouse); 315750 (Rat)

Alternate Names

FLJ33279; KLG; klotho gamma; Klotho/lactase-phlorizin hydrolase-related protein; KLPH; KLPHKL lactase phlorizin hydrolase; Lactase like Protein; Lactase-like Protein; lactase-like; LCTL