The TGF‑ beta family includes several related proteins (70‑80% sequence homology) from mammalian, avian, or Xenopus systems that are now designated TGF‑ beta 1, TGF‑ beta 2, TGF‑ beta 1.2, TGF‑ beta 3, TGF‑ beta 4, and TGF‑ beta 5. These proteins are secreted by cells in the form of an inactive complex, referred to as latent TGF-beta, that consists of TGF‑ beta associated non-covalently with a Latency-associated peptide (LAP). These two proteins are synthesized as a single pro-peptide that is cleaved in a post Golgi compartment prior to secretion. Different TGF‑ beta family members are naturally associated with their own distinct LAPs.
Human LAP (TGF-beta 1) Antibody
R&D Systems | Catalog # MAB2463
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Flow Cytometry, CyTOF-ready
Cited:
Immunohistochemistry-Frozen, Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 27232
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Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human LAP (TGF-beta 1)
Leu30-Arg278 (Cys33Ser)
Accession # P01137
Leu30-Arg278 (Cys33Ser)
Accession # P01137
Specificity
Detects human LAP (TGF-beta 1) in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) TGF‑ beta 1, rhTGF‑ beta 2, rhTGF‑ beta 1.2, rhTGF‑ beta 3, or rhTGF‑ alpha.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Human LAP (TGF-beta 1) Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: Human platelets
Sample: Human platelets
Reviewed Applications
Read 1 review rated 5 using MAB2463 in the following applications:
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: LAP (TGF-beta 1)
Long Name
Latency-associated Peptide
Alternate Names
LAP (TGFbeta 1)
Entrez Gene IDs
7040 (Human)
Gene Symbol
TGFB1
UniProt
Additional LAP (TGF-beta 1) Products
Product Documents for Human LAP (TGF-beta 1) Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human LAP (TGF-beta 1) Antibody
For research use only
Citations for Human LAP (TGF-beta 1) Antibody
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Customer Reviews for Human LAP (TGF-beta 1) Antibody (1)
5 out of 5
1 Customer Rating
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Application: ELISASample Tested: Cell Culture SupernatesSpecies: HumanVerified Customer | Posted 06/28/2017
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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FAQs for Human LAP (TGF-beta 1) Antibody
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Q: How do you isolate human platelets for flow cytometry, using Human LAP (TGF-beta 1) Antibody, Catalog # MAB2463, and its conjugates?
A: We obtain the platelets from blood plasma by collection the top layer after spinning down whole blood. We proceed with a normal surface stain protocol; no special buffers are required.
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