Human LAP (TGF-beta 1) Antibody

(5 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human LAP (TGF-beta 1) in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) TGF‑ beta 1, rhTGF‑ beta 2, rhTGF‑ beta 1.2, rhTGF‑ beta 3, or rhTGF‑ alpha.
  • Source
    Monoclonal Mouse IgG1 Clone # 27232
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human LAP (TGF-beta 1)
    Leu30-Arg278 (Cys33Ser)
    Accession # P01137
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    Human platelets
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LAP (TGF-beta 1)

The TGF‑ beta family includes several related proteins (70‑80% sequence homology) from mammalian, avian, or Xenopus systems that are now designated TGF‑ beta 1, TGF‑ beta 2, TGF‑ beta 1.2, TGF‑ beta 3, TGF‑ beta 4, and TGF‑ beta 5. These proteins are secreted by cells in the form of an inactive complex, referred to as latent TGF-beta, that consists of TGF‑ beta associated non-covalently with a Latency-associated peptide (LAP). These two proteins are synthesized as a single pro-peptide that is cleaved in a post Golgi compartment prior to secretion. Different TGF‑ beta family members are naturally associated with their own distinct LAPs.

  • Long Name:
    Latency-associated Peptide
  • Entrez Gene IDs:
    7040 (Human)
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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Species
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Sample Type
  1. Overexpression of TGF-ß 1 gene induces cell surface localized glucose-regulated protein 78-associated latency-associated peptide/TGF-ß.
    Authors: Oida T, Weiner HL
    J. Immunol., 2012;185(6):3529-35.
    Species: Human
    Sample Type: whole cells
    Application: Flow Cytometry
  2. Induction of latency-associated peptide (transforming growth factor-beta(1)) expression on CD4+ T cells reduces Toll-like receptor 4 ligand-induced tumour necrosis factor-alpha production in a transforming growth factor-beta-dependent manner.
    Authors: Boswell S, Sharif S, Alisa A, Pereira SP, Williams R, Behboudi S
    Immunology, 2011;133(3):278-87.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow Cytometry
  3. HLA-DR+ leukocytes acquire CD1 antigens in embryonic and fetal human skin and contain functional antigen-presenting cells.
    Authors: Schuster C, Vaculik C, Fiala C, Meindl S, Brandt O, Imhof M, Stingl G, Eppel W, Elbe-Burger A
    J. Exp. Med., 2009;206(1):169-81.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Frozen
  4. Human anti-inflammatory macrophages induce Foxp3+ GITR+ CD25+ regulatory T cells, which suppress via membrane-bound TGFbeta-1.
    Authors: Savage ND, De Boer T, Walburg KV, Joosten SA, van Meijgaarden K, Geluk A, Ottenhoff TH
    J. Immunol., 2008;181(3):2220-6.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow Cytometry
  5. TGF-beta 1 plays an important role in the mechanism of CD4+CD25+ regulatory T cell activity in both humans and mice.
    Authors: Kitani A, Fuss I, Pedersen A, Nawata H
    J. Immunol., 2004;172(2):834-42.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow Cytometry
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

Ctrl MAB002 94
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Mouse IgG1 Isotype Control

Ctrl MAB002R
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Secondary Antibodies
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Mouse IgG Horseradish Peroxidase-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL007 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 4
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 5
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Goat Anti-Mouse IgG Antibody

WB AF007 3
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Mouse IgG Horseradish Peroxidase-conjugated Antibody

WB HAF018 2
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 2
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Goat Anti-Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 2
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002
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Goat Anti-Mouse IgG Fc Antibody

G-202-C 1
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001
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Donkey Anti-Mouse IgG (H+L) Antibody

D-201-C-ABS2
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