Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all LARG Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human LARG
Ile154-Leu575
Accession # Q9NZN5

Specificity

Detects endogenous human LARG in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human LARG Antibody

Detection of Human LARG antibody by Western Blot.

Detection of Human LARG by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human LARG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4737) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for LARG at approximately 200 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.

Applications for Human LARG Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LARG

LARG (Leukemia-associated RhoGEF, also known as Rho guanine nucleotide exchange factor 12) is a 173 kDa member of the DH‑domain containing RhoGEF family of molecules. It is ubiquitously expressed, and known to interact with semaphorin-activated B class plexins, thereby activating Rho-dependent pathways. Human LARG is 1544 amino acids (aa) in length. It contains one PZD domain that binds to plexin (aa 72‑151), an RGSL region (aa 368‑558), and a RhoGEF domain (aa 788‑975). There are at least two potential splice forms. One shows a deletion of aa 48‑66 with an 8 aa poly-Lys substitution for the C-terminal 960 amino acids. A second shows an insertion of 9 aa between Thr1093 and Asp1094, accompanied by a 6 aa substitution for the C-terminal 131 amino acids. Over aa 154‑575, human LARG is 90% aa identical to mouse LARG.

Long Name

Leukemia-associated RhoGEF

Alternate Names

ARHGC, ARHGEF12

Entrez Gene IDs

23365 (Human); 69632 (Mouse); 367072 (Rat)

Gene Symbol

ARHGEF12

UniProt

Q9NZN5

Additional LARG Products

Product Documents for Human LARG Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human LARG Antibody

For research use only

Citations for Human LARG Antibody

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