Human Latent TGF-beta  bp2/LTBP-2 Antibody

Catalog # Availability Size / Price Qty
AF3850
AF3850-SP
Detection of Human Latent TGF‑ beta  bp2/LTBP‑2 by Western Blot.
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Human Latent TGF-beta  bp2/LTBP-2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Latent TGF‑ beta  bp2/LTBP‑2 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse (rm) LTBP-2 is observed, and less than 1% cross-reactivity with recombinant human LTBP-1 and rmLTBP-4 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Latent TGF‑ beta  bp2/LTBP‑2
Gln36-Glu1821
Accession # Q14767
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human Latent TGF‑ beta  bp2/LTBP‑2 by Western Blot. View Larger

Detection of Human Latent TGF‑ beta  bp2/LTBP‑2 by Western Blot. Western blot shows lysates of HFL1 human fetal lung fibroblast cell line and human heart tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Latent TGF‑ beta  bp2/LTBP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3850) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Latent TGF‑ beta  bp2/LTBP‑2 at approximately 210-255 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Latent TGF-beta bp2/LTBP-2

LTBP-2 (Latent TGF-beta Binding Protein 2; also C14orf141) is a 220-250 kDa secreted glycoprotein member of the LTBP family of proteins. In SDS-PAGE and under reducing conditions, LTBP-2 runs at 220-250 kDa; under nonreducing conditions, LTBP-2 runs between 205-220 kDa. Although it is described as being a TGF-beta binding protein, it does not possess the ability to bind covalently to TGF-beta. Instead, it appears to bind to heparin sulfate moieties on syndecan-4 and perlecan, plus fibrillin-1 and fibulin-5, suggesting a role in both elastic fiber formation and the maintenance of basement membrane integrity. In addition, LTPB-2 is now known to form a complex with PC5/6A intracellularly, and upon secretion, dampen PC5 activation in the extracellular environment. Cells known to express LTBP-2 include lens and nonpigmented ciliary epithelium, fibroblasts, vascular smooth muscle and chondrocytes. Mature human LTBP-2 is 1786 amino acids (aa) in length (aa 36-1821). It possesses three heparin-binding regions (aa 94-115, 232-249, and 344-354), plus 20 EGF-like domains (aa 187-1818) that contain four interspersed TGF-beta type binding segments. Over aa 36-1821, human LTBP-2 shares 80% aa sequence identity with mouse LTBP-2.

Entrez Gene IDs
4053 (Human)
Alternate Names
C14orf141; GLC3D; Latent TGFbeta bp2; Latent TGF-beta bp2; latent transforming growth factor beta binding protein 2; latent-transforming growth factor beta-binding protein 2; LTBP2; LTBP-2; LTBP3chromosome 14 open reading frame 141; MSTP031

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FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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