Key Product Details

Species Reactivity

Human

Applications

Western Blot, Intracellular Staining by Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all LAX1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human LAX1 isoform 1
Asn136-Glu398
Accession # Q8IWV1

Specificity

Detects human LAX1 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human LAX1 Antibody

Detection of Human LAX1 antibody by Western Blot.

Detection of Human LAX1 by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human LAX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4706) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for LAX1 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of LAX1 antibody in Jurkat Human Cell Line antibody by Flow Cytometry.

Detection of LAX1 in Jurkat Human Cell Line by Flow Cytometry.

Jurkat human acute T cell leukemia cell line were stained with Goat Anti-Human LAX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4706, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Applications for Human LAX1 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: Jurkat human acute T cell leukemia cell line fixed with paraformaldehyde and permeabilized with saponin

Western Blot

1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and K562 human chronic myelogenous leukemia cell line

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: LAX1

LAX1 (Linker for activation of X cells) is a 70 kDa member of a small group of T cell transmembrane (TM) adaptor proteins. It is upregulated on both T and B cells after activation, and likely downmodulates both BCR and TCR signaling. Human LAX1 is 398 amino acids (aa) in length. Although its predicted MW is 44 kDa, it runs anomalously at 70 kDa in SDS-PAGE. It is a type III (no signal sequence) TM protein that contains a 37 aa N-terminal extracellular region and a 340 aa cytoplasmic domain (aa 59‑398). There are two potential isoform variants. One has an alternate start site at Met77, while a second shows a 13 aa substitution for aa 1‑29. Over aa 136‑398, human LAX1 is 47% aa identical to mouse LAX1.

Long Name

Lymphocyte Transmembrane Adaptor 1

Alternate Names

LAT-like membrane associated protein, LAXFLJ20340, Linker for activation of X cells, lymphocyte transmembrane adapter 1, lymphocyte transmembrane adaptor 1, Membrane-associated adapter protein LAX

Entrez Gene IDs

54900 (Human); 240754 (Mouse); 498232 (Rat)

Gene Symbol

LAX1

UniProt

Q8IWV1

Additional LAX1 Products

Product Documents for Human LAX1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human LAX1 Antibody

For research use only

Customer Reviews for Human LAX1 Antibody

There are currently no reviews for this product. Be the first to review Human LAX1 Antibody and earn rewards!

Have you used Human LAX1 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies