Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot, Neutralization

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all LBP Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human LBP
Ala26-Val481
Accession # AAD21962

Specificity

Detects human LBP in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse LBP is observed and less than 1% cross-reactivity with recombinant human BPI is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human LBP Antibody

Detection of Human LBP by Western Blot

Detection of Human LBP by Western Blot

Western blot validation of proteins overexpressed in cancer sera. (A) Western blots of 50 μg of medium and low abundance proteins from each non-cancer (N1-N6) and cancer (C1-C6) serum pool depleted by the IgY12 HPLC column were probed with the antibodies to ECM1, LBP1, and alpha-1 antitrypsin. (B) A replicate of the PVDF blot was stained for total protein with colloidal gold as a control for loading/transfer. Bracket indicates the approximate region of the blot where proteins of interest would be expected. Image collected and cropped by CiteAb from the following open publication (https://proteomesci.biomedcentral.com/articles/10.1186/1477-5956-8-31), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human LBP by Western Blot

Detection of Human LBP by Western Blot

Western blot validation of proteins overexpressed in cancer sera. (A) Western blots of 50 μg of medium and low abundance proteins from each non-cancer (N1-N6) and cancer (C1-C6) serum pool depleted by the IgY12 HPLC column were probed with the antibodies to ECM1, LBP1, and alpha-1 antitrypsin. (B) A replicate of the PVDF blot was stained for total protein with colloidal gold as a control for loading/transfer. Bracket indicates the approximate region of the blot where proteins of interest would be expected. Image collected and cropped by CiteAb from the following open publication (https://proteomesci.biomedcentral.com/articles/10.1186/1477-5956-8-31), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human LBP Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human LBP (Catalog # 870-LP)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LBP

Lipopolysaccharide-binding protein (LBP), a 58 kDa glycoprotein synthesized in hepatocytes, belongs to a family of lipid-binding proteins which includes bactericidal/permeability increasing protein (BPI), phospholipid ester transfer protein (PLTP), and cholesterol ester transfer protein (CETP). LBP binds to the lipid A portion of lipopolysaccharide (LPS) to facilitate the process of LPS monomerization, catalyze the binding of LPS monomers to CD14, and promote LPS-induced immune response. LBP is present at low concentrations in normal human serum and may increase up to 30-fold during the acute phase response. Studies indicate that LBP also acts catalytically in the transfer of LPS to HDL, thus accelerating LPS detoxification. In addition, LBP and soluble CD14 can also function in phospholipid transport.

References

  1. Hailman, E. et al. (1994) J. Exp. Med. 179:269.
  2. Jack, R.S. et al. (1997) Nature 389:742.
  3. Schumann, R.R. et al. (2000) Chem. Immunol. 74:42.

Long Name

Lipopolysaccharide-binding Protein

Alternate Names

lipopolysaccharide binding protein, lipopolysaccharide-binding protein, LPS-binding protein, MGC22233

Entrez Gene IDs

3929 (Human); 16803 (Mouse)

Gene Symbol

LBP

UniProt

AAD21962

Additional LBP Products

Product Documents for Human LBP Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human LBP Antibody

For research use only

Citations for Human LBP Antibody

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