Human LDL R Antibody MAB2148-100: R&D Systems

Human LDL R Antibody

(6 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human LDL R in ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) LDL R, recombinant human LRP-5, or rmLRP-6 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 472413
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human LDL R
    Ala22-Arg788
    Accession # P01130
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human LDL R (Catalog # 2148-LD) under non-reducing conditions only
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • Immunoprecipitation
    25 µg/mL
    Conditioned cell culture medium spiked with Recombinant Human LDL R (Catalog # 2148‑LD), see our available Western blot detection antibodies
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
    • Human LDL R Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human LDL R Antibody (Catalog # MAB2148)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human LDL R Biotinylated Antibody (Catalog # BAF2148)
  • ELISA Standard
     
    Recombinant Human LDL R Protein (Catalog # 2148-LD)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of LDL R in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human LDL R Monoclonal Antibody (Catalog # MAB2148, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by PE-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LDL R

The low density lipoprotein receptor (LDL R) is the founding member of the LDL R family of scavenger receptors (1, 2). This family contains transmembrane molecules that are characterized by the presence of EGF repeats, complement-like repeats, and YWTD motifs that form beta -propellers. Although members of the family were originally thought to be endocytic receptors, it is now clear that some members interact with adjacent cell-surface molecules, expanding their range of activities (2). Human LDL R is synthesized as an 860 amino acid (aa) precursor that contains a 21 aa signal sequence, a 767 aa extracellular region, a 22 aa transmembrane segment and a 50 aa cytoplasmic tail (3). The extracellular region is complex. It consists of seven N-terminal complement-like cysteine-rich repeats that bind ligand. Cysteine residues in this region participate in intrachain disulfide bonds. This region is followed by three EGF-like repeats with a beta -propeller YWTD containing motif. The EGF-like repeats are responsible for ligand bonding and dissociation. Finally, there is a 50 aa membrane proximal Ser/Thr-rich region that serves as a carbohydrate attachment point (1, 3, 4). There is extensive O-linked and modest N-linked glycosylation. Thus the receptor’s predicted molecular weight of 93 kDa is increased to a native molecular weight of 120‑160 kDa (3, 4). Within the 50 aa cytoplasmic tail, there is an NPXY motif that links the receptor to clathrin pits (1). The extracellular region of human LDL R is 51% aa identical to the extracellular region of human VLDL R, and 79% aa identical to the extracellular region of mouse LDL R. LDL R is constitutively expressed and binds apoB of LDL and apoE of VLDL (5). It is responsible for clearing 70% of plasma LDL in liver (5). Mutations in the LDL R gene cause the autosomal dominant disorder, familial hypercholesterolemia (6).

  • References:
    1. Strickland, D.K. et al. (2002) Trends Endocrinol. Metab. 13:66.
    2. Nykjaer, A. and T.E. Willnow (2002) Trends Cell Biol. 12:273.
    3. Yamamoto, T. et al. (1984) Cell 39:27.
    4. Davis, C.G. et al. (1986) J. Biol. Chem. 261:2828.
    5. Defesche, J.C. (2004) Semin. Vasc. Med. 4:5.
    6. Varret, M. et al. (2008) Clin Genet. 73:1.
  • Long Name:
    Low Density Lipoprotein Receptor
  • Entrez Gene IDs:
    3949 (Human); 16835 (Mouse); 300438 (Rat)
  • Alternate Names:
    FH; FHC; LDL R; LDL receptor; LDLCQ2; LDLR; low density lipoprotein receptor; low-density lipoprotein receptor class A domain-containing protein 3; low-density lipoprotein receptor
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Applications
Sample Type
  1. Proprotein convertase subtilisin/Kexin type 9 (PCSK9) single domain antibodies are potent inhibitors of LDL receptor degradation
    Authors: Elodie Weider
    J Biol Chem, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC - Not specified
  2. Endophilin marks and controls a clathrin-independent endocytic pathway.
    Authors: Boucrot E, Ferreira A, Almeida-Souza L, Debard S, Vallis Y, Howard G, Bertot L, Sauvonnet N, McMahon H
    Nature, 2015;517(7535):460-5.
    Application: Functional Assay
  3. Allergen challenge in vivo alters rhinovirus-induced chemokine secretion from human airway macrophages.
    Authors: Karta, Maya R, Wickert, Lisa E, Curran, Colleen, Gavala, Monica L, Denlinger, Loren C, Gern, James E, Bertics, Paul J
    J Allergy Clin Immunol, 2014;133(4):1227-30.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Mystery solved: VSV-G-LVs do not allow efficient gene transfer into unstimulated T cells, B cells, and HSCs because they lack the LDL receptor.
    Authors: Amirache F, Levy C, Costa C, Mangeot P, Torbett B, Wang C, Negre D, Cosset F, Verhoeyen E
    Blood, 2014;123(9):1422-4.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. Characterization of proprotein convertase subtilisin/kexin type 9 (PCSK9) trafficking reveals a novel lysosomal targeting mechanism via amyloid precursor-like protein 2 (APLP2).
    Authors: DeVay, Rachel M, Shelton, David L, Liang, Hong
    J Biol Chem, 2013;288(15):10805-18.
    Species: Human
    Sample Type: Cell Lysates
    Application: ELISA Development
  6. Dissection of the endogenous cellular pathways of PCSK9-induced low density lipoprotein receptor degradation: evidence for an intracellular route.
    Authors: Poirier S, Mayer G, Poupon V, McPherson PS, Desjardins R, Ly K, Asselin MC, Day R, Duclos FJ, Witmer M, Parker R, Prat A, Seidah NG
    J. Biol. Chem., 2009;284(42):28856-64.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
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