Human LIGHT/TNFSF14 Antibody Summary
Accession # O43557
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of LIGHT/TNFSF14 in Human CD3+ T cells by Flow Cytometry. Human CD3+ T cells treated with 1 ng/mL PMA and 1 μg/mL Calcium Ionomycin for 72 hours were stained with (A) Rabbit Anti-Human LIGHT/TNFSF14 Monoclonal Antibody (Catalog # MAB6643) or (B) isotype control antibody (Catalog # MAB1050) followed by anti-Rabbit IgG APC-conjugated secondary antibody (Catalog # F0111) and Mouse Anti-Human CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB100P). View our protocol for Staining Membrane-associated Proteins.
Cell Proliferation Induced by LIGHT/TNFSF14 and Neutralization by Human LIGHT/TNFSF14 Antibody. Recombinant Human LIGHT/TNFSF14 (Catalog # 664-LI) induces proliferation in the HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human LIGHT/TNFSF14 (20 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Human LIGHT/TNFSF14 Monoclonal Antibody (Catalog # MAB6644). The ND50 is typically 6-48 ng/mL.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Human LIGHT, also known as TNFSF14, is a type II membrane protein that is a member of the TNF superfamily. LIGHT is an acronym which stands for "is homologous to lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes". LIGHT has also been called HVEM-L and LT-gamma. LIGHT is a 240 amino acid (aa) protein that contains a 37 aa cytoplasmic domain, a 22 aa transmembrane region, and a 181 aa extracellular domain. Similar to other TNF ligand family members, LIGHT is predicted to assemble as a homotrimer. LIGHT is produced by activated T cells and was first identified by its ability to compete with HSV glycoprotein D for HVEM binding. LIGHT has also been shown to bind to the lymphotoxin beta receptor (LT beta R) and the decoy receptor (DcR3/TR6). LIGHT overexpression in tumor cells induces apoptosis, which can be enhanced by IFN-gamma.
- Mauri, D.N. et al. (1998) Immunity 8:21.
- Zhai, Y. et al. (1998) J. Clin. Invest. 102:1142.
- Harrop, J.A. et al. (1998) J. Biol. Chem. 273:27548.
- Yu, K-Y. et al. (1999) J. Biol. Chem. 274:13733.
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