Human LIGHT/TNFSF14 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB6643-100
MAB6643-SP
Detection of LIGHT/TNFSF14 in Human CD3+ T cells by Flow Cytometry.
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Human LIGHT/TNFSF14 Antibody Summary

Species Reactivity
Human
Specificity
Detects human LIGHT/TNFSF14 in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2323C
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human LIGHT/TNFSF14
Asp74-Val240
Accession # O43557
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Neutralization
Measured by its ability to neutralize LIGHT/TNFSF14-induced proliferation in the HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci USA 87:1323. The Neutralization Dose (ND50) is typically 6-48 ng/mL in the presence of 20 ng/mL Recombinant Human LIGHT/TNFSF14.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of LIGHT/TNFSF14 in Human CD3+ T cells by Flow Cytometry. View Larger

Detection of LIGHT/TNFSF14 in Human CD3+ T cells by Flow Cytometry. Human CD3+ T cells treated with 1 ng/mL PMA and 1 μg/mL Calcium Ionomycin for 72 hours were stained with (A) Rabbit Anti-Human LIGHT/TNFSF14 Monoclonal Antibody (Catalog # MAB6643) or (B) isotype control antibody (Catalog # MAB1050) followed by anti-Rabbit IgG APC-conjugated secondary antibody (Catalog # F0111) and Mouse Anti-Human CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB100P). View our protocol for Staining Membrane-associated Proteins.

Neutralization Cell Proliferation Induced by LIGHT/TNFSF14 and Neutral­ization by Human LIGHT/TNFSF14 Antibody. View Larger

Cell Proliferation Induced by LIGHT/TNFSF14 and Neutral­ization by Human LIGHT/TNFSF14 Antibody. Recombinant Human LIGHT/TNFSF14 (Catalog # 664-LI) induces proliferation in the HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human LIGHT/TNFSF14 (20 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Human LIGHT/TNFSF14 Monoclonal Antibody (Catalog # MAB6644). The ND50 is typically 6-48 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LIGHT/TNFSF14

Human LIGHT, also known as TNFSF14, is a type II membrane protein that is a member of the TNF superfamily. LIGHT is an acronym which stands for "is homologous to lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes". LIGHT has also been called HVEM-L and LT-gamma. LIGHT is a 240 amino acid (aa) protein that contains a 37 aa cytoplasmic domain, a 22 aa transmembrane region, and a 181 aa extracellular domain. Similar to other TNF ligand family members, LIGHT is predicted to assemble as a homotrimer. LIGHT is produced by activated T cells and was first identified by its ability to compete with HSV glycoprotein D for HVEM binding. LIGHT has also been shown to bind to the lymphotoxin beta receptor (LT beta R) and the decoy receptor (DcR3/TR6). LIGHT overexpression in tumor cells induces apoptosis, which can be enhanced by IFN-gamma.

References
  1. Mauri, D.N. et al. (1998) Immunity 8:21.
  2. Zhai, Y. et al. (1998) J. Clin. Invest. 102:1142.
  3. Harrop, J.A. et al. (1998) J. Biol. Chem. 273:27548.
  4. Yu, K-Y. et al. (1999) J. Biol. Chem. 274:13733.
Long Name
TNF Ligand Superfamily Member 14
Entrez Gene IDs
8740 (Human); 50930 (Mouse)
Alternate Names
CD258 antigen; CD258; delta transmembrane LIGHT; Herpes virus entry mediator ligand; Herpesvirus entry mediator ligand; HVEM-L; HVEMLherpesvirus entry mediator-ligand; ligand for herpesvirus entry mediator; LIGHT; LIGHTherpesvirus entry mediator A; LTg; TNFSF14; TR2; tumor necrosis factor (ligand) superfamily, member 14; tumor necrosis factor ligand superfamily member 14; tumor necrosis factor receptor-like 2; tumor necrosis factor superfamily member LIGHT

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