Human LIGHT, also known as TNFSF14, is a type II membrane protein that is a member of the TNF superfamily. LIGHT is an acronym which stands for "is homologous to lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes". LIGHT has also been called HVEM-L and LT-gamma. LIGHT is a 240 amino acid (aa) protein that contains a 37 aa cytoplasmic domain, a 22 aa transmembrane region, and a 181 aa extracellular domain. Similar to other TNF ligand family members, LIGHT is predicted to assemble as a homotrimer. LIGHT is produced by activated T cells and was first identified by its ability to compete with HSV glycoprotein D for HVEM binding. LIGHT has also been shown to bind to the lymphotoxin beta receptor (LT beta R) and the decoy receptor (DcR3/TR6). LIGHT overexpression in tumor cells induces apoptosis, which can be enhanced by IFN-gamma.
Human LIGHT/TNFSF14 Antibody
R&D Systems | Catalog # MAB6643
Recombinant Monoclonal Antibody.
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Neutralization, Flow Cytometry, CyTOF-ready
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2323C
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human LIGHT/TNFSF14
Asp74-Val240
Accession # O43557
Asp74-Val240
Accession # O43557
Specificity
Detects human LIGHT/TNFSF14 in direct ELISAs.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human LIGHT/TNFSF14 Antibody
Detection of LIGHT/TNFSF14 in Human CD3+ T cells by Flow Cytometry.
Human CD3+ T cells treated with 1 ng/mL PMA and 1 µg/mL Calcium Ionomycin for 72 hours were stained with (A) Rabbit Anti-Human LIGHT/TNFSF14 Monoclonal Antibody (Catalog # MAB6643) or (B) isotype control antibody (Catalog # MAB1050) followed by anti-Rabbit IgG APC-conjugated secondary antibody (Catalog # F0111) and Mouse Anti-Human CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB100P). View our protocol for Staining Membrane-associated Proteins.
Cell Proliferation Induced by LIGHT/TNFSF14 and Neutralization by Human LIGHT/TNFSF14 Antibody.
Recombinant Human LIGHT/TNFSF14 (Catalog # 664-LI) induces proliferation in the HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human LIGHT/TNFSF14 (20 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Human LIGHT/TNFSF14 Monoclonal Antibody (Catalog # MAB6643). The ND50 is typically 6-48 ng/mL.Applications for Human LIGHT/TNFSF14 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Human CD3+ T cells treated with PMA and Calcium Ionomycin
Sample: Human CD3+ T cells treated with PMA and Calcium Ionomycin
Neutralization
Measured by its ability to neutralize LIGHT/TNFSF14-induced proliferation in the HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci USA 87:1323. The Neutralization Dose (ND50) is typically 6-48 ng/mL in the presence of 20 ng/mL Recombinant Human LIGHT/TNFSF14.
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: LIGHT/TNFSF14
References
- Mauri, D.N. et al. (1998) Immunity 8:21.
- Zhai, Y. et al. (1998) J. Clin. Invest. 102:1142.
- Harrop, J.A. et al. (1998) J. Biol. Chem. 273:27548.
- Yu, K-Y. et al. (1999) J. Biol. Chem. 274:13733.
Long Name
TNF Ligand Superfamily Member 14
Alternate Names
CD258, TNFSF14
Gene Symbol
TNFSF14
UniProt
Additional LIGHT/TNFSF14 Products
Product Documents for Human LIGHT/TNFSF14 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human LIGHT/TNFSF14 Antibody
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Liperfluo
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
