Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Flow Cytometry, CyTOF-ready

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all LILRB5/CD85c/LIR-8 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human LILRB5/CD85c/LIR-8
Arg18-His456
Accession # O75023

Specificity

Detects human LILRB5/CD85c/LIR-8 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) LILRA4/ILT7 is observed and less than 15% cross-reactivity with rhILT3 and rhILT5 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human LILRB5/CD85c/LIR-8 Antibody

Detection of LILRB5/CD85c/LIR-8 antibody in Human peripheral blood monocytes antibody by Flow Cytometry.

Detection of LILRB5/CD85c/LIR-8 in Human peripheral blood monocytes by Flow Cytometry.

Human peripheral blood monocytes were stained with Goat Anti-Human LILRB5/CD85c/LIR-8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3065, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Detection of Human LILRB5/CD85c/LIR-8 by Western Blot.

Western blot shows lysates of Human bone marrow. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human LILRB5/CD85c/LIR-8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3065) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for LILRB5/CD85c/LIR-8 at approximately 64 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Applications for Human LILRB5/CD85c/LIR-8 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Human peripheral blood monocytes and granulocytes

Western Blot

0.1-0.5 µg/mL
Sample: Recombinant Human LILRB5/CD85c/LIR-8, human bone marrow

Reviewed Applications

Read 1 review rated 5 using AF3065 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LILRB5/CD85c/LIR-8

Human LIR-8 (also known as CD85c and LILRB5) is a 587 amino acid transmembrane protein of the family of Leukocyte Immunoglobin-like Receptors. LIR-8 has a long N-terminus extracellular domain with four C2-type lg-like domains, and a short cytoplasmic tail with two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) typically involved in modulation of cellular responses. Human LIR-8 is expressed in natural killer (NK) cells. There are at least two other known isoforms, one with 53 aa longer cytoplasmic domain, the other with a 100 aa deletion affecting one of the lg-like extracellular domains.

Long Name

Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM And ITIM Domains), Member 5

Alternate Names

CD85c, LIR-8, LIR8

Entrez Gene IDs

10990 (Human); 102129427 (Cynomolgus Monkey)

Gene Symbol

LILRB5

UniProt

O75023

Additional LILRB5/CD85c/LIR-8 Products

Product Documents for Human LILRB5/CD85c/LIR-8 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human LILRB5/CD85c/LIR-8 Antibody

For research use only

Citations for Human LILRB5/CD85c/LIR-8 Antibody

Customer Reviews for Human LILRB5/CD85c/LIR-8 Antibody (1)

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  • Human LILRB5/CD85c/LIR-8 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum
    Species: Human
    Verified Customer | Posted 11/29/2022
    Human LILRB5/CD85c/LIR-8 Antibody AF3065

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