Human LILRB5/CD85c/LIR-8 Antibody

AF3065 4 Citations Datasheet / COA / SDS

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AF3065
AF3065-SP

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Detection of LILRB5/CD85c/LIR-8 in Human peripheral blood monocytes by Flow Cytometry.

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Citations (4)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human LILRB5/CD85c/LIR-8 Antibody Summary

Species Reactivity

Human

Specificity

Detects human LILRB5/CD85c/LIR-8 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) LILRA4/ILT7 is observed and less than 15% cross-reactivity with rhILT3 and rhILT5 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human LILRB5/CD85c/LIR-8
Arg18-His456
Accession # O75023

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human LILRB5/CD85c/LIR-8

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry

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Detection of LILRB5/CD85c/LIR-8 in Human peripheral blood monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Goat Anti-Human LILRB5/CD85c/LIR-8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3065, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LILRB5/CD85c/LIR-8

Human LIR-8 (also known as CD85c and LILRB5) is a 587 amino acid transmembrane protein of the family of Leukocyte Immunoglobin-like Receptors. LIR-8 has a long N-terminus extracellular domain with four C2-type lg-like domains, and a short cytoplasmic tail with two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) typically involved in modulation of cellular responses. Human LIR-8 is expressed in natural killer (NK) cells. There are at least two other known isoforms, one with 53 aa longer cytoplasmic domain, the other with a 100 aa deletion affecting one of the lg-like extracellular domains.

Long Name

Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM And ITIM Domains), Member 5

Entrez Gene IDs

10990 (Human); 102129427 (Cynomolgus Monkey)

Alternate Names

CD85 antigen-like family member C; CD85c antigen; CD85c; Leukocyte immunoglobulin-like receptor 8; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains); LILRB5; LIR8; LIR-8; LIR8CD85C; LIR-8leukocyte immunoglobulin-like receptor subfamily B member 5; member 5

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human LILRB5/CD85c/LIR-8 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Coexpression of CD163 and CD141 identifies human circulating IL-10-producing dendritic cells (DC-10)
Authors: M Comi, D Avancini, F Santoni de, M Villa, MJ Uyeda, M Floris, D Tomasoni, A Bulfone, MG Roncarolo, S Gregori
Cell. Mol. Immunol., 2019;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
The extracellular interactome of the human adenovirus family reveals diverse strategies for immunomodulation
Nat Commun, 2016;7(0):11473.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
The Leukocyte Immunoglobulin-Like Receptor Family Member LILRB5 Binds to HLA-Class I Heavy Chains.
Authors: Zhang Z, Hatano H, Shaw J, Olde Nordkamp M, Jiang G, Li D, Kollnberger S
PLoS ONE, 2015;10(6):e0129063.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Inhibitory receptors bind ANGPTLs and support blood stem cells and leukaemia development.
Authors: Zheng J, Umikawa M, Cui C, Li J, Chen X, Zhang C, Hyunh H, Kang X, Silvany R, Wan X, Ye J, Canto AP, Chen SH, Wang HY, Ward ES, Zhang CC
Nature, 2012;485(7400):656-60.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry