Human LILRB5/CD85c/LIR-8 Antibody

Name: Human LILRB5/CD85c/LIR-8 Antibody
Brand: R&D Systems

(3 citations)

Datasheet / CoA / SDS

Flow Cytometry

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Product Details

Citations (3)

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Species Reactivity

Human
Specificity

Detects human LILRB5/CD85c/LIR-8 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) LIR-4/ILT6 is observed, and approximately 10% cross-reactivity with rhILT5, rhLIR-5/ILT3, rhLIR-6, and rhLILRA5 is observed, and less than 5% cross-reactivity with rhILT2, rhILT4, and rhLIR-7 is observed.
Source

Polyclonal Goat IgG
Purification

Antigen Affinity-purified
Immunogen

Mouse myeloma cell line NS0-derived recombinant human LILRB5/CD85c/LIR-8
Arg18-His456
Accession # O75023
Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label

Unconjugated

Product Datasheets

Product Datasheet

COA

Certificate of Analysis Lookup

Certificate of Analysis Request Form

SDS

Applications

Recommended
Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human LILRB5/CD85c/LIR-8
Flow Cytometry

2.5 µg/10⁶ cells

See below
CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry

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Detection of LILRB5/CD85c/LIR-8 in Human peripheral blood monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Goat Anti-Human LILRB5/CD85c/LIR-8 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3065, filled histogram) or control antibody (Catalog # AB‑108‑C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LILRB5/CD85c/LIR-8

Human LIR-8 (also known as CD85c and LILRB5) is a 587 amino acid transmembrane protein of the family of Leukocyte Immunoglobin-like Receptors. LIR-8 has a long N-terminus extracellular domain with four C2-type lg-like domains, and a short cytoplasmic tail with two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) typically involved in modulation of cellular responses. Human LIR-8 is expressed in natural killer (NK) cells. There are at least two other known isoforms, one with 53 aa longer cytoplasmic domain, the other with a 100 aa deletion affecting one of the lg-like extracellular domains.

Long Name:

Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM And ITIM Domains), Member 5

Entrez Gene IDs:

10990 (Human)

Alternate Names:

CD85 antigen-like family member C; CD85c antigen; CD85c; Leukocyte immunoglobulin-like receptor 8; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains); LILRB5; LIR8; LIR-8; LIR8CD85C; LIR-8leukocyte immunoglobulin-like receptor subfamily B member 5; member 5

Related Research Areas

Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species	Applications	Sample Type
The extracellular interactome of the human adenovirus family reveals diverse strategies for immunomodulation Nat Commun, 2016;7(0):11473. Species: Human Sample Type: Whole Cells Application: Flow The Leukocyte Immunoglobulin-Like Receptor Family Member LILRB5 Binds to HLA-Class I Heavy Chains. Authors: Zhang Z, Hatano H, Shaw J, Olde Nordkamp M, Jiang G, Li D, Kollnberger S PLoS ONE, 2015;10(6):e0129063. Species: Human Sample Type: Whole Cells Application: Flow Inhibitory receptors bind ANGPTLs and support blood stem cells and leukaemia development. Authors: Zheng J, Umikawa M, Cui C, Li J, Chen X, Zhang C, Hyunh H, Kang X, Silvany R, Wan X, Ye J, Canto AP, Chen SH, Wang HY, Ward ES, Zhang CC Nature, 2012;485(7400):656-60. Species: Human Sample Type: Whole Cells Application: Flow

FAQs

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Isotype Controls

Description Application Cat# Citations Images

Normal Goat IgG Control

Ctrl AB-108-C 210

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Human LILRB5/CD85c/LIR-8 Antibody

Certificate of Analysis Lookup

Certificate of Analysis Lookup

Certificate of Analysis Request Form

FAQs

Normal Goat IgG Control

Reconstitution Buffer 1 (PBS)

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